Summary
The filiform hairs, mechanoreceptors of Gryllus, pass through six developmental stages during the last larval stage. The cytoplasm of their sense cells suggests intensive synthesis of protein for cellular metabolism and intercytoplasmic exchange of material via glial evaginations. Ultrahistochemical tests demonstrated acid phosphatase in the lysosomes as well as in components of the Golgi apparatus. There was no significant change in the appearance of the sense cell cytoplasm, indicating a maintained functional state also during molting. The new cuticular apparatus is formed after apolysis by the three enveloping cells. Formation of the replacement hairs is initiated by a cytoplasmic outgrowth of the trichogen cell. During morphogenesis of the new hair, the microtubules serve as a cytoskeleton and probably control the flow of vesicles, which contain phenol oxidase, also demonstrated in the Golgi apparatus, and are incorporated into the new cuticle. Bundles of microfibrils are involved in the surface sculpturing of the replacement hair. The trichogen cell also forms a number of structural elements, e.g. the “cup” and “strut” marked geometric peculiarities of which indicate that they are important in the spatial orientation of the dendrite and thus also in transduction. Reduction of the apical cell membrane of the tormogen cell after apolysis permits unrestricted growth of the new hair into the exuvial space. The tormogen cell participates in the formation of the joint membrane, parts of the socket and the articulation of the hair.
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References
Altner, H., Thies, G.:Reizleitende Strukturen und Ablauf der Häutung an Sensillen einer euedaphischen Collembolenart. Z. Zellforsch. 129, 196–216 (1972)
Ashhurst, D.E., Costin, N.: Insect mucosubstances. II. The mucosubstances of the central nervous system. Histochem. J. 3, 297–310 (1971)
Atema, J.: Microtubule theory of sensory transduction. J. theor. Biol. 38, 131–190 (1973)
Bardele, C.F.: A microtubule model for ingestion and transport in the suctorian tentacle. Z. Zellforsch. 126, 116–137 (1972)
Blaney, W.M., Chapman, R.F.: The fine structure of the terminal sensilla of the maxillary palps of Schistocerca gregaria (Forskål) (Orthoptera, Acrididae). Z. Zellforsch. 99, 74–97 (1969)
Blaney, W.M., Chapman, R.F., Cook, A.G.: The structure of the terminal sensilla on the maxillary palps of Locusta migratoria (L.), and changes associated with moulting. Z. Zellforsch. 121, 48–68 (1971)
Bray, D.: Surface movement during the growth of a single expanded neuron. Proc. nat. Acad. Sci. (Wash.) 65, 905 (1970)
Dakshayani, K., Mathad, S.B.: A comparative study of growth, development and survival of the cricket Plebeiogryllus guttiventris Walker reared singly and in groups. Experientia (Basel) 29, 978–979 (1973)
Davidoff, M.S., Galabov, G.P.: Lysosomen and lysosomale Enzyme im Zentralnervensystem der Ratte. In: Progress in histochemistry and cytochemistry 6. (W. Graumann, Z. Loyda, A.G.E. Pearse and T.H. Schiebler, eds.), S. 1–64. Stuttgart: Gustav Fischer 1974
Ernst, K.-D.: Die Feinstruktur von Riechsensillen auf der Antenne von Necrophorus (Coleoptera). Z. Zellforsch. 94, 72–102 (1969)
Ernst, K.-D.: Die Ontogenie der basiconischen Riechsensillen auf der Antenne von Necrophorus (Coleoptera). Z. Zellforsch. 129, 217–236 (1972)
Foelix, R.F., Chu-Wang, I-Wu, Beck, L.: Fine structure of tarsal sensory organs in the whip spider Admetus pumilo (Amblypygi, Arachnida). Tissue & Cell 7, 331–346 (1975)
Gnatzy, W.: The ultrastructure of the thread-hairs on the cerci of the cockroach Periplaneta americana L.: The intermoult phase. J. Ultrastruct. Res. 54, 124–134 (1976)
Gnatzy, W., Schmidt, K.: Die Feinstruktur der Sinneshaare auf den Cerci von Gryllus bimaculatus Deg. (Saltatoria, Gryllidae). I. Fadenund Keulenhaare. Z. Zellforsch. 122, 190–209 (1971)
Gnatzy, W., Schmidt, K.: Die Feinstruktur der Sinneshaare auf den Cerci von Gryllus bimaculatus Deg. (Saltatoria, Gryllidae). IV. Die Häutung der kurzen Borstenhaare. Z. Zellforsch. 126, 223–239 (1972a)
Gnatzy, W., Schmidt, K.: Die Feinstruktur der Sinneshaare auf den Cerci von Gryllus bimaculatus Deg. (Saltatoria, Gryllidae). V. Die Häutung der langen Borstenhaare an der Cercusbasis. J. Microscopie 14, 75–84 (1972b)
Gnatzy, W., Tautz, J.: Sensitivity of an insect mechanoreceptor during moulting. Physiol. Entomol. 2 (1977)
Gordon, G.B., Miller, L.R., Bensch, K.G.: Studies on the intracellular digestive process in mammalian tissue culture cells. J. Cell Biol. 25, 41–55 (1965)
Greenstein, M.E.: The ultrastructure of developing wings in the giant silkmoth, Hyalophora cecropia. II. Scale-forming and socket-forming cells. J. Morph. 136, 23–52 (1972)
Harris, D.J., Mill, P.J.: The ultrastructure of chemoreceptor sensilla in Ciniflo (Araneida, Arachnida). Tissue & Cell 5, 679–689 (1973)
Hayes, W.F.: Fine structure of the chemoreceptor sensillum in Limulus. J. Morph. 133, 205–240 (1971)
Jenkin, P.M., Hinton, H.E.: Apolysis in arthropod moulting cycle. Nature (Lond.) 211, 871 (1966)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965)
Keil, Th.: Sinnesorgane auf den Antennen von Lithobius forficatus L. (Myriapoda, Chilopoda). I. Die Funktionsmorphologie der “Sensilla trichodea”. Zoomorphologie 84, 77–102 (1976)
Kreuzberg, G.W., Hager, H.: Electron microscopical demonstration of acid phosphatase activity in the central nervous system. Histochemie 6, 254–259 (1966)
Kushida, H., Fujita, K.: Simultaneous double staining. VI. Internat. Congr. EM, pp. 39–40, Kyoto 1966
Larink, O.: Entwicklung und Feinstruktur der Schuppen bei Lepismatiden und Machiliden (Insecta, Zygentoma und Archaeognatha). Zool. Jb. Anat. 95, 252–293 (1976)
Lawrence, P.A.: Development and determination of hairs and bristles in the milkweed bug, Oncopeltus fasciatus (Lygaeidae, Hemiptera). J. Cell Sci. 1, 475–498 (1966)
Locke, H.: The structure of an epidermal cell during the development of the protein epicuticle and the uptake of molting fluid in an insect. J. Morph. 127, 7–40 (1969)
Locke, M., Krishman, N.: The distribution of phenoloxidases and polyphenols during cuticle formation. Tissue & Cell 3, 103–126 (1971)
Locke, M., Krishman, N.: The formation of the ecdysial droplets and the ecdysial membrane in an insect. Tissue & Cell 5, 441–450 (1973)
Moran, D.T., Rowley III, J.C., Zill, S.N., Varela, F.G.: The mechanism of sensory transduction in a mechanoreceptor. Functional stages in campaniform sensilla during the molting cycle. J. Cell Biol. 71, 832–847 (1976)
Moran, D.T., Varela, F.G.: Microtubules and sensory transduction. Proc. nat. Acad. Sci. (Wash.) 68, 757–760 (1971)
Overton, J.: Microtubules and microfibrils in morphogenesis of the scale cells of Ephestia kühniella. J. Cell Biol. 29, 293–305 (1966)
Overton, J.: The fine structure of developing bristles in wild type and mutant Drosophila melanogaster. J. Morph. 122, 367–380 (1967)
Porter, K.R.: Cytoplasmic microtubules and their function. In: Ciba Foundation Symp. on Principles of Biomolecular Organization (G.E.W. Wolstenholme and M. O'Conner, eds.), pp. 303–345. London: Churchill 1966
Raekallio, I.: Enzyme histochemistry of wound healing. In: Progress in histochemistry and cytochemistry I (W. Graumann, Z. Loyda, A.G.E. Pearse, and T.H. Schiebler, eds.), pp. 51–152. Stuttgart-Portland (USA): Gustav Fischer 1970
Ribbert, D.: Relation of puffing to bristle and footpad differentiation in Calliphora and Sarcophaga. In: Results and problems in cell differentiation (W. Beermann, ed.), Vol. 4, pp. 153–179. Berlin-Heidelberg-New York: Springer 1972
Richard, G.: L'innervation sensorielle pendant les mues chez les insectes. Bull. Soc. Zool. France 77, 99–106 (1952)
Schmidt, K.: Vergleichende morphologische Untersuchungen an Mechanorezeptoren der Insekten. Verh. Dtsch. Zool. Ges. 66, 15–25 (1973)
Schmidt, K.: Die Mechanorezeptoren im Pedicellus der Eintagsfliegen (Insecta, Ephemeroptera). Z. Morph. Tiere 78, 193–220 (1974)
Schmidt, K., Gnatzy, W.: Die Feinstruktur der Sinneshaare auf den Cerci von Gryllus bimaculatus Deg. (Saltatoria, Gryllidae). II. Die Häutung der Faden-und Keulenhaare. Z. Zellforsch. 122, 210–226 (1971)
Sihler, H.: Die Sinnesorgane an den Cerci der Insekten. Zool. Jb. Anat. 45, 519–580 (1924)
Slautterback, D.B.: Cytoplasmic microtubules. I. Hydra. J. Cell Biol. 18, 367–388 (1963)
Smith, D.S.: The trophic role of glial cells in insect ganglia. In: Insects and physiology (J.W.L. Beament and J.E. Treherne, eds.), pp. 184–193. Edinburgh: Oliver and Boyd 1967
Thurm, U.: Untersuchungen zur funktionellen Organisation sensorischer Zellverbände. Verh. Dtsch. Zool. Ges. Köln 1970, pp. 79–88 (1970)
Thurm, U.: The generation of receptor potentials in epithelial receptors. In: Olfaction and taste IV. (D. Schneider, ed.), pp. 95–101. Stuttgart: Wiss. Verlagsgesellschaft 1972
Thurm, U.: Basics of the generation of receptor potentials in epidermal mechanoreceptors of insects. In: Mechanoreception (J. Schwartzkopff, ed.). Opladen: Westdeutscher Verlag 1974
Walcott, C., Salpeter, M.M.: The effect of molting upon the vibration of the spider (Achaearanea tepidariorum). J. Morph. 119, 383–399 (1966)
Wolff, H.H.: Über die Entwicklung der Enzymmusters der Rattenretina. Histochmie 17, 11–29 (1969)
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Supported by the Deutsche Forschungsgemeinschaft. The author thanks Mrs. G. Thomas for drawing the diagrams, and Miss I. Grossman and Mrs. M. Ullmann for technical assistance
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Gnatzy, W. Development of the filiform hairs on the cerci of Gryllus bimaculatus Deg. (Saltatoria, Gryllidae). Cell Tissue Res. 187, 1–24 (1978). https://doi.org/10.1007/BF00220615
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DOI: https://doi.org/10.1007/BF00220615