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Studies on the midgut of Centropages typicus (Copepod, Calanoid)

I. Structural and ultrastructural data

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Summary

From a structural study of the midgut of Centropages typicus, three main zones presenting different cellular associations may be defined.

The ultrastructural study carried out allows us to distinguish several cell types. The four principal cell categories (E, R, F and B) show a more or less marked resemblance with those identified in the Malacostraceans, especially in the hepatopancreas of Decapods. The three types R, F and B, which are subdivided according to their localization (R and R′) or their developing stages (F1, F2; B1 to B5), are recognized by the following characteristics: R-cells with smooth endoplasmic reticulum and high microvilli (zones I and III); F-cells with rough endoplasmic reticulum and short or spherical microvilli (zones I and II); B-cells with a large vacuolar apparatus made up of lysosome-like dense bodies associated with vacuoles originating from pinocytosis (zone II).

From the comparisons of morphological, ultrastructural and histochemical results concerning Centropages typicus with author's data, we propose and discuss these functions for the three principal cell types: synthesis and secretion of pre-digestive enzymes (F1 and F2-cells); enzyme synthesis, intracellular digestion and extrusion (B1 to B5-cells); absorption (R and R′-cells).

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References

  • Alikhan, M.A.: The physiology of the woodlouse, Porcellio laevis Latreille. I. Studies on the gut epithelium cytology and its relation to the maltase secretion. Canad. J. Zool. 47, 65–75 (1969)

    Google Scholar 

  • Bunt, A.H.: An ultrastructural study of the hepatopancreas of Procambarus clarkii (Girard)(Decapoda, Astacidea). Crustaceana 15, 282–288 (1968)

    Google Scholar 

  • Burighel, P., Milanesi, C: Fine structure of the gastric epithelium of the Ascidian Botryllus schlosseri. Vacuolated and zymogenic cells. Cell Tiss. Res. 145, 541–555 (1973)

    Google Scholar 

  • Clifford, B., Witkus, E.R.: The fine structure of the hepatopancreas of the woodlouse Oniscus asellus. J. Morph. 135, 335–350 (1971)

    Google Scholar 

  • Daems, W.T., Wisse, E., Brederoo, P.: Electron microscopy of the vacuolar apparatus. In: Lysosomes in biology and pathology 1, pp. 64–112 (J.T. Dingle, ed.). In: Frontiers of biology 29. London and New-York: Neuberger and Tatum 1969

    Google Scholar 

  • Dakin, W.D.: Note on the alimentary canal and food of the Copepoda. Int. Rev. ges. Hydrobiol. Hydrogr. 1, 772–782 (1908)

    Google Scholar 

  • Dall, W.: The functional anatomy of the digestive tract of a shrimp, Metapenaeus bennetae Racek and Dall. Austr. J. Zool. 15, 699–714 (1967)

    Google Scholar 

  • Davis, L.E., Burnett, A.L.: A study of growth and cell differentiation in the hepatopancreas of the crayfish. Develop. Biol. 10, 122–153 (1964)

    Google Scholar 

  • Donadey, C.: Contribution à l'étude ultrastructurale et histophysiologique des caecums digestifs des Crustacés Isopodes. Thèse Doct. Etat, 157p. 40pl. Marseille: Univ. Provence 1973

    Google Scholar 

  • Durfort, M.: Consideraciones sobre la estructura y ultraestructura del epitelio intestinal de Mytilicola intestinalis Steuer. Actas prim. Cent. R. Soc. esp. Hist, nat., 109–120 (1971)

  • Fahrenbach, W.H.: The biology of a Harpacticoid Copepod. Cellule 62, 303–376 (1962)

    Google Scholar 

  • Gabe, M.: Techniques histologiques. Paris: Masson & Cie. 1968

    Google Scholar 

  • George, M J.: The anatomy of the crab Portunus sanguinolentus Herbst. V. Digestive system. J. Madras Univ. 35–36 B, 83–93 (1965–1966)

  • Georgi, R.: Feinstruktur peritrophischer Membranen von Crustaceen. Z. Morph. Tiere 65 (3), 225–273 (1969a)

    Google Scholar 

  • Georgi, R.: Bildung peritrophischer Membranen von Decapoden. Z. Zellforsch. 99, 570–607 (1969b)

    Google Scholar 

  • Giraudie, J., Lauthier, M.: Recherche des phosphatases alcalines dans l'épiderme au cours du développement précoce des membres de deux Anoures (Rana pipiens et R. dalmatina) et d'un Urodèle (Pleurodeles waltlii). C.R. Acad. Sci. (Paris) 275, 2547–2549 (1972)

    Google Scholar 

  • Guieysse, A.: Etude des organes digestifs chez les Crustacés. Arch. Anat. Micr. 9, 343–494 (1907)

    Google Scholar 

  • Hirsch, G.C., Jacobs, W.: Der Arbeitsrhythmus der Mitteldarmdrüse von Astacus leptodactylus. I. Methodik und Technik: Der Beweis der Periodizität. Z. wiss. Biol. 8, 102–144 (1928)

    Google Scholar 

  • Hirsch, G.C., Jacobs, W.: Der Arbeitsrhythmus der Mitteldarmdrüse von Astacus leptodactylus. II. Wachstum als primärer Faktor des Rhythmus eines polyphasischen organischen Sekretion-systems. Z. wiss. Biol. 12, 524–558 (1930)

    Google Scholar 

  • Jacobs, W.: Untersuchungen über die Cytologie der Sekretbildung in der Mitteldarmdrüse von Astacus leptodactylus. Z. Zellforsch. 8, 1–62 (1928)

    Google Scholar 

  • Jones, D.A., Babbage, P.C., King, P.E.: Studies on digestion and the fine structure of digestive caeca in Eurydice pulchra (Crustacea, Isopoda). Mar. Biol. 2, 311–320 (1969)

    Google Scholar 

  • Kurosumi, K.: Electron microscopic analysis of the secretion mechanism. Int. Rev. Cytol. 11, 1–117 (1961)

    Google Scholar 

  • Loizzi, R.F.: Interpretation of crayfish hepatopancreas function based on fine structural analysis of epithelial cell lines and muscular network. Z. Zellforsch. 113, 420–440 (1971)

    Google Scholar 

  • Lowe, E.: The anatomy of a marine Copepod Calanus finmarchicus (Gunneras). Trans. roy. Soc. Edinb. 58, 561–603 (1935)

    Google Scholar 

  • Mabillot, S.: Contribution à l'étude histophysiologique de l'appareil digestif de Gammarus pulex L. Arch. Zool. exp. gen. 92, 20–38 (1955)

    Google Scholar 

  • Marino, D., Onesto, E.: La nutrizione dei Saffirinide (Copepodi). Struttura del canale alimentare di Sapphirina angusta Dana. Pubbl. Staz. zool. Napoli 38, 355–363 (1970)

    Google Scholar 

  • Marshall, S.M., Orr, A.P.: The biology of a marine Copepod, Calanus finmarchicus (Gunnerus). Edinburgh: Oliver and Boyd 1955

    Google Scholar 

  • Martoja, R., Martoja, M.: Initiation aux techniques de l'histologie animale. Paris: Masson & Cie 1967

    Google Scholar 

  • Miyawaki, M., Sasaki, N.: A preliminary report on uptake of Ca45 by the hepatopancreas of crayfish, Procambarus clarkii. Kumamoto J. Sci. 5 (2), 170–172 (1961)

    Google Scholar 

  • Morris, I.G.: Gamma globulin absorption in the newborn. Handbook of Physiology, Sect. G, III, 1491–1512 (1968)

  • Nath, C.N., Krishna Pillai, N.: On the alimentary canal of Spelacomysis longipes (Crustacea Mysidacea). J. zool. Soc. India 23 (2): 95–108 (1971)

    Google Scholar 

  • Ong, J.E., Lake, P.S.: The ultrastructural morphology of the midgut diverticulum of the Calanoid Copepod Calanus helgolandicus (Claus) (Crustacea). Austr. J. Zool. 18, 9–20 (1969)

    Google Scholar 

  • Park, T.S.: The biology of a Calanoid Copepod, Epilabidocera amphitrites Mc Murrish. Cellule 66, 129–251 (1966)

    Google Scholar 

  • Raymont, J.E.G., Krishnaswamy, S., Woodhouse, M.A., Griffin, R.L.: Studies on the fine structure of Copepoda. Observations on Calanus finmarchicus (Gunnerus). Proc. roy. Soc. Edinb. B 185 (1081), 409–424 (1974)

    Google Scholar 

  • Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

    Google Scholar 

  • Schmitz, E.H., Schultz, T.W.: Digestive anatomy of terrestrial Isopods: Armadillium vulgare and A.nasatum. Amer. Mid. Nat. 82 (1), 163–181 (1969)

    Google Scholar 

  • Smith, J.M., Nadakavukarem, M.J., Hetzel, H.R.: Light and electron microscopy of the hepatopancreas of the Isopod Asellus intermedius. Cell Tiss. Res. 163, (3), 403–410 (1975)

    Google Scholar 

  • Stanier, J.E., Woodhouse, M.A., Griffin, R.L.: The fine structure of the hepatopancreas of Carcinus maenas (L.) (Decapoda Brachyura). Crustaceana 14, 56–66 (1968)

    Google Scholar 

  • Susumu, I.: Anatomic structure of the gastric mucosa. Handbook of Physiology, Sect. G, II, 705–741 (1967)

  • Talbot, P., Clark, W.H., Lawrence, A.L.: Fine structure of the midgut epithelium in the developing brown shrimp, Penaeus aztecus. J. Morph. 138 (4), 467–486 (1972)

    Google Scholar 

  • Thomas, N.W.: Mucus secreting cells from the alimentary canal of Ciona intestinalis (L.). J. mar. biol. Ass. U.K. 50, 429–438 (1970a)

    Google Scholar 

  • Thomas, N.W.: Morphology of cell types from the gastric epithelium of Ciona intestinalis (L.). J. mar. biol. Ass. U.K. 50, 737–746 (1970b)

    Google Scholar 

  • Travis, D.F.: The molting cycle of the spiny lobster Palinurus argus Latre'ule. 2. Pre-ecdysial histological and histochemical changes in the hepatopancreas and integumental tissues. Biol. Bull. 108, 88–112 (1955)

    Google Scholar 

  • Travis, D.F.: The molting cycle of the spiny lobster Palinurus argus Latreille. 4. Post-ecdysial histological and histochemical changes in the hepatopancreas and integumental tissues. Biol. Bull. 113, 451–479 (1957)

    Google Scholar 

  • Weel, P.B. van: Processes of secretion, restitution and resorption in gland of midgut of Atya spinipes Newport. Physiol. Zool. 28, 40–54 (1955)

    Google Scholar 

  • Wessing, A.: Die Funktion der Malpighischen Gefäße. In: K.E. Wohlfarth-Bottermann, Funktionelle und morphologische Organisation der Zelle: Sekretion und Exkretion. S. 228–268. Z. wiss. Konf. Ges. dtsch. Naturforsch. Ärzte, Schloß Reinhardsbrunn bei Friedrichsroda 1964. Berlin-Heidelberg-New York: Springer 1965

    Google Scholar 

  • Yoshikoshi, K.: On the structure and function of the alimentary canal of Tigriopusjaponicus(Copepoda, Harpacticoida). I. Histological structure. Bull. jap. Soc. sc. Fish. 41 (9), 929–935 (1975)

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biologie Animale (Plancton), Université de Provence, Place Victor-Hugo, 13331, Marseille Cedex 3, France

    J. Arnaud, M. Brunet & J. Mazza

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  1. J. Arnaud
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  2. M. Brunet
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  3. J. Mazza
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Additional information

We would like to thank Pr. M.-L. Furnestin for her advice in the preparation of this paper, Mrs. R. Kollmann for her technical assistance and Mr. J.D. Lee for his help in the translation of the manuscript

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Arnaud, J., Brunet, M. & Mazza, J. Studies on the midgut of Centropages typicus (Copepod, Calanoid). Cell Tissue Res. 187, 333–353 (1978). https://doi.org/10.1007/BF00224375

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