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Immunohistochemical localization of secretory proteins in the endometrial epithelium of the rabbit

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Summary

Proteins of uterine fluid and lung homogenates of the rabbit were separated by gel and ion exchange chromatography. Purified protein fractions were used for immunisation and antiserum production. By means of several absorptions, six monospecific antisera against uteroglobin and five other proteins were obtained. Using immunohistochemistry, four of them could be localised in the uterine epithelium from oestrus and the first and the seventh day post coitum, and also in the blastocyst. The present study indicates the involvement of different endometrial cells in the synthesis and release of the various proteins of uterine secretion.

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References

  • Arthur, A.T., Cowan, B.D., Daniel, J.C. Jr.: Steroid binding to blastokinin. Fertil. Steril. 23, 85–92 (1972)

    Google Scholar 

  • Alger, M., Milgrom, E.: Progesterone-induced messenger RNA. Translation, purification, and preliminary characterization of uteroglobin mRNA. J. Biol. Chem. 252, 5412–5418 (1977)

    Google Scholar 

  • Beato, M., Beier, H.M.: Characteristics of the purified uteroglobin-like protein from rabbit lung. J. Reprod. Fertil. 53, 305–314 (1978)

    Google Scholar 

  • Beato, M., Nieto, A.: Translation of the mRNA for rabbit uteroglobin in cell-free systems. Eur. J. Biochem. 64, 15–25 (1976)

    Google Scholar 

  • Beier, H.M.: Das Proteinmilieu im Blutserum, Uterus und in den Blastocysten des Kaninchens vor der Nidation. Colloquium Biochemie der Morphogenese, Dt. Forschungsgemeinschaft, Konstanz 1966

    Google Scholar 

  • Beier, H.M.: Biochemisch-entwicklungsphysiologische Untersuchungen am Proteinmilieu für die Blastocystenentwicklung des Kaninchens (Oryctolagus cuniculus). Zool. Jb. Anat. 85, 72–190 (1968)

    Google Scholar 

  • Beier, H.M.: Hormonal Stimulation of protease inhibitor activity in endometrial secretion during early pregnancy. Acta Endocrinol. (Kbh.) 63, 141–149 (1970)

    Google Scholar 

  • Beier, H.M.: Uterine secretion protein patterns under endogenous and exogenous hormonal influences. In: Current Topics in Pathology (A. Gropp and K. Benirschke, Eds.). 62, 105–120. Springer-Verlag: Berlin-Heidelberg-New York (1976)

  • Beier, H.M., Kirchner, C., Mootz, U.: Uteroglobin-like antigen in the pulmonary epithelium and secretion of the lung. Cell Tissue Res. 190, 15–25 (1978)

    Google Scholar 

  • Beier, H.M., Kühnel, W.: Morphologische und biochemische Untersuchungen der Sekretion von Samenblasen. Verh. Anat. Ges. 68, Lausanne 1973, Anat. Anz. (Egh.) 136, 231–245 (1974)

    Google Scholar 

  • Bullock, D.W.: Progesterone induction of messenger RNA and protein synthesis in rabbit uterus. Ann. N.Y. Acad. Science. 286, 260–272 (1977)

    Google Scholar 

  • Denker, H.W.: Enzym-Topochemie von Frühentwicklung und Implantation des Kaninchens. III. Proteasen. Histochemie 25, 344–360 (1971)

    Google Scholar 

  • Denker, H. W.: Trophoblastic factors involved in lysis of blastocyst coverings and in implantation of the rabbit: Observations on inversely orientated blastocysts. J. Embryol. Exp. Morph. 32, 739–748 (1974)

    Google Scholar 

  • Denker, H.W.: Implantation: The role of proteinases, and blockage of implantation by proteinase inhibitors. Adv. in Anatomy, Embryology and Cell Biology (A. Brodal, W. Hild, J. van Limborgh, R. Ortmann, T.H. Schiebler, G. Töndury, E. Wolff, Eds.). Vol. 53 Fasc. 5. Springer-Verlag: Berlin-Heidelberg-New York (1977)

    Google Scholar 

  • Dies, R.: Hormonale Beeinflussung des Sekretproteinmusters und der Keimentwicklung im Genitaltrakt von Oryctolagus cuniculus. Zool. Jb. Physiol. 77, 360–441 (1973)

    Google Scholar 

  • El-Banna, A.A., Daniel, J.C. Jr.: Stimulation of rabbit blastocysts in vitro by progesterone and uterine proteins in combination. Fertil. Steril. 23, 101–104 (1972)

    Google Scholar 

  • Gulyas, B.J., Daniel, J.C. Jr., Krishnan, R.S.: Incorporation of labelled nucleosides in vitro by rabbit and mink blastocysts in the presence of blastokinin or serum. J. Reprod. Fertil. 20, 255–262 (1969)

    Google Scholar 

  • Johnson, M.H.: The distribution of a blastokinin-like uterine protein studied by immune fluorescence. Fertil. Steril. 23, 929–939 (1972)

    Google Scholar 

  • Kirchner, C.: Untersuchungen an uterusspezifischen Glykoproteinen während der frühen Gravidität des Kaninchens (Oryctolagus cuniculus). Wilhelm Roux' Archiv 164, 97–133 (1969)

    Google Scholar 

  • Kirchner, C.: Einfluß von Choriongonadotrophin auf die Sekretion eines uterusspezifischen Proteins des Kaninchens. Acta Endocrinol. (Kbh.) 68, 394–400 (1971)

    Google Scholar 

  • Kirchner, C.: Immune histologic studies on the synthesis of a uterine-specific protein in the rabbit and its passage through the blastocyst coverings. Fertil. Steril. 23, 131–136 (1972 a)

    Google Scholar 

  • Kirchner, C.: Uterine protease activities and lysis of the blastocyst covering in the rabbit. J. Embryol. Exp. Morph. 28, 177–183 (1972 b)

    Google Scholar 

  • Kirchner, C.: Interferenzkontrastmikroskopische Untersuchungen über die Lysis der Keimhüllen beim Kaninchen. Cytobiologie 7, 437–441 (1973)

    Google Scholar 

  • Kirchner, C.: Uteroglobin in the rabbit: I. Intracellular localization in the oviduct, uterus and preimplantation blastocyst. Cell Tissue Res. 170, 415–424 (1976 a)

    Google Scholar 

  • Kirchner, C.: Uteroglobin in the rabbit: II. Intracellular localization in the uterus after hormone treatment. Cell Tissue Res. 170, 425–434 (1976 b)

    Google Scholar 

  • Kirchner, C., Schroer, H.G.: Uterine secretion-like proteins in the seminal plasma of the rabbit. J. Reprod. Fertil. 47, 325–330 (1976)

    Google Scholar 

  • Krishnan, R.S., Daniel, J.C. Jr.: “Blastokinin”: Inducer and regulator of blastocyst development in the rabbit uterus. Science 158, 490–492 (1967)

    Google Scholar 

  • Maurer, R.R., Beier, H.M.: Uterine proteins and development in vitro of rabbit preimplantation embryos. J. Reprod. Fertil. 48, 33–41 (1976)

    Google Scholar 

  • Noske, I.G., Feigelson, M.: Immunological evidence of Uteroglobin (blastokinin) in the male reproductive tract and in nonreproductive ductal tissues and their secretions. Biol. Reprod. 15, 704–713 (1976)

    Google Scholar 

  • Petzoldt, U., Dames, W., Gottschewski, G.H.M., Neuhoff, V.: Das Proteinmuster in frühen Entwicklungsstadien des Kaninchens. Cytobiologie 5, 272–280 (1972)

    Google Scholar 

  • Rahman, S.S.U., Billiar, R.B., Little, B.: Induction of uteroglobin in rabbits by progestogens, estradiol-17β and ACTH. Biol. Reprod. 12, 305–314 (1975)

    Google Scholar 

  • Sacco, A.G., Shivers, C.A.: Localisation of tissue-specific antigens in the rabbit ovary, oviduct and uterus by the fluorescent antibody technique. J. Reprod. Fertil. 32, 415–420 (1973)

    Google Scholar 

  • Schwick, H.G.: Chemisch-entwicklungsphysiologische Beziehungen von Uterus zu Blastocyste des Kaninchens (Oryctolagus cuniculus). Wilhelm Roux' Archiv 156, 283–343 (1965)

    Google Scholar 

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Supported by grant Ki 154/6-7 from the Deutsche Forschungsgemeinschaft

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Kirchner, C. Immunohistochemical localization of secretory proteins in the endometrial epithelium of the rabbit. Cell Tissue Res. 199, 25–36 (1979). https://doi.org/10.1007/BF00237725

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