Summary
Proteins of uterine fluid and lung homogenates of the rabbit were separated by gel and ion exchange chromatography. Purified protein fractions were used for immunisation and antiserum production. By means of several absorptions, six monospecific antisera against uteroglobin and five other proteins were obtained. Using immunohistochemistry, four of them could be localised in the uterine epithelium from oestrus and the first and the seventh day post coitum, and also in the blastocyst. The present study indicates the involvement of different endometrial cells in the synthesis and release of the various proteins of uterine secretion.
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References
Arthur, A.T., Cowan, B.D., Daniel, J.C. Jr.: Steroid binding to blastokinin. Fertil. Steril. 23, 85–92 (1972)
Alger, M., Milgrom, E.: Progesterone-induced messenger RNA. Translation, purification, and preliminary characterization of uteroglobin mRNA. J. Biol. Chem. 252, 5412–5418 (1977)
Beato, M., Beier, H.M.: Characteristics of the purified uteroglobin-like protein from rabbit lung. J. Reprod. Fertil. 53, 305–314 (1978)
Beato, M., Nieto, A.: Translation of the mRNA for rabbit uteroglobin in cell-free systems. Eur. J. Biochem. 64, 15–25 (1976)
Beier, H.M.: Das Proteinmilieu im Blutserum, Uterus und in den Blastocysten des Kaninchens vor der Nidation. Colloquium Biochemie der Morphogenese, Dt. Forschungsgemeinschaft, Konstanz 1966
Beier, H.M.: Biochemisch-entwicklungsphysiologische Untersuchungen am Proteinmilieu für die Blastocystenentwicklung des Kaninchens (Oryctolagus cuniculus). Zool. Jb. Anat. 85, 72–190 (1968)
Beier, H.M.: Hormonal Stimulation of protease inhibitor activity in endometrial secretion during early pregnancy. Acta Endocrinol. (Kbh.) 63, 141–149 (1970)
Beier, H.M.: Uterine secretion protein patterns under endogenous and exogenous hormonal influences. In: Current Topics in Pathology (A. Gropp and K. Benirschke, Eds.). 62, 105–120. Springer-Verlag: Berlin-Heidelberg-New York (1976)
Beier, H.M., Kirchner, C., Mootz, U.: Uteroglobin-like antigen in the pulmonary epithelium and secretion of the lung. Cell Tissue Res. 190, 15–25 (1978)
Beier, H.M., Kühnel, W.: Morphologische und biochemische Untersuchungen der Sekretion von Samenblasen. Verh. Anat. Ges. 68, Lausanne 1973, Anat. Anz. (Egh.) 136, 231–245 (1974)
Bullock, D.W.: Progesterone induction of messenger RNA and protein synthesis in rabbit uterus. Ann. N.Y. Acad. Science. 286, 260–272 (1977)
Denker, H.W.: Enzym-Topochemie von Frühentwicklung und Implantation des Kaninchens. III. Proteasen. Histochemie 25, 344–360 (1971)
Denker, H. W.: Trophoblastic factors involved in lysis of blastocyst coverings and in implantation of the rabbit: Observations on inversely orientated blastocysts. J. Embryol. Exp. Morph. 32, 739–748 (1974)
Denker, H.W.: Implantation: The role of proteinases, and blockage of implantation by proteinase inhibitors. Adv. in Anatomy, Embryology and Cell Biology (A. Brodal, W. Hild, J. van Limborgh, R. Ortmann, T.H. Schiebler, G. Töndury, E. Wolff, Eds.). Vol. 53 Fasc. 5. Springer-Verlag: Berlin-Heidelberg-New York (1977)
Dies, R.: Hormonale Beeinflussung des Sekretproteinmusters und der Keimentwicklung im Genitaltrakt von Oryctolagus cuniculus. Zool. Jb. Physiol. 77, 360–441 (1973)
El-Banna, A.A., Daniel, J.C. Jr.: Stimulation of rabbit blastocysts in vitro by progesterone and uterine proteins in combination. Fertil. Steril. 23, 101–104 (1972)
Gulyas, B.J., Daniel, J.C. Jr., Krishnan, R.S.: Incorporation of labelled nucleosides in vitro by rabbit and mink blastocysts in the presence of blastokinin or serum. J. Reprod. Fertil. 20, 255–262 (1969)
Johnson, M.H.: The distribution of a blastokinin-like uterine protein studied by immune fluorescence. Fertil. Steril. 23, 929–939 (1972)
Kirchner, C.: Untersuchungen an uterusspezifischen Glykoproteinen während der frühen Gravidität des Kaninchens (Oryctolagus cuniculus). Wilhelm Roux' Archiv 164, 97–133 (1969)
Kirchner, C.: Einfluß von Choriongonadotrophin auf die Sekretion eines uterusspezifischen Proteins des Kaninchens. Acta Endocrinol. (Kbh.) 68, 394–400 (1971)
Kirchner, C.: Immune histologic studies on the synthesis of a uterine-specific protein in the rabbit and its passage through the blastocyst coverings. Fertil. Steril. 23, 131–136 (1972 a)
Kirchner, C.: Uterine protease activities and lysis of the blastocyst covering in the rabbit. J. Embryol. Exp. Morph. 28, 177–183 (1972 b)
Kirchner, C.: Interferenzkontrastmikroskopische Untersuchungen über die Lysis der Keimhüllen beim Kaninchen. Cytobiologie 7, 437–441 (1973)
Kirchner, C.: Uteroglobin in the rabbit: I. Intracellular localization in the oviduct, uterus and preimplantation blastocyst. Cell Tissue Res. 170, 415–424 (1976 a)
Kirchner, C.: Uteroglobin in the rabbit: II. Intracellular localization in the uterus after hormone treatment. Cell Tissue Res. 170, 425–434 (1976 b)
Kirchner, C., Schroer, H.G.: Uterine secretion-like proteins in the seminal plasma of the rabbit. J. Reprod. Fertil. 47, 325–330 (1976)
Krishnan, R.S., Daniel, J.C. Jr.: “Blastokinin”: Inducer and regulator of blastocyst development in the rabbit uterus. Science 158, 490–492 (1967)
Maurer, R.R., Beier, H.M.: Uterine proteins and development in vitro of rabbit preimplantation embryos. J. Reprod. Fertil. 48, 33–41 (1976)
Noske, I.G., Feigelson, M.: Immunological evidence of Uteroglobin (blastokinin) in the male reproductive tract and in nonreproductive ductal tissues and their secretions. Biol. Reprod. 15, 704–713 (1976)
Petzoldt, U., Dames, W., Gottschewski, G.H.M., Neuhoff, V.: Das Proteinmuster in frühen Entwicklungsstadien des Kaninchens. Cytobiologie 5, 272–280 (1972)
Rahman, S.S.U., Billiar, R.B., Little, B.: Induction of uteroglobin in rabbits by progestogens, estradiol-17β and ACTH. Biol. Reprod. 12, 305–314 (1975)
Sacco, A.G., Shivers, C.A.: Localisation of tissue-specific antigens in the rabbit ovary, oviduct and uterus by the fluorescent antibody technique. J. Reprod. Fertil. 32, 415–420 (1973)
Schwick, H.G.: Chemisch-entwicklungsphysiologische Beziehungen von Uterus zu Blastocyste des Kaninchens (Oryctolagus cuniculus). Wilhelm Roux' Archiv 156, 283–343 (1965)
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Kirchner, C. Immunohistochemical localization of secretory proteins in the endometrial epithelium of the rabbit. Cell Tissue Res. 199, 25–36 (1979). https://doi.org/10.1007/BF00237725
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DOI: https://doi.org/10.1007/BF00237725