Summary
Transport of ferritin and horseradish peroxidase from the 3rd ventricle to the median eminence was examined in rats during the perinatal life, the time when functional interrelations between hypothalamus and hypophysis are established. Protein tracers injected into the lateral ventricle are adsorbed on the apical surface of the tanycyte, mainly on its protrusions or in indentations. On the 18th day of prenatal life a few small bleblike protrusions are observed. After birth microvilli appear. In time their concentration increases to result in an increase of adsorbed substances. They are taken up by smooth and coated pinocytotic vesicles and transported to the basal portion of the cell or to the intercellular space bypassing junctional complexes. In addition to pinocytotic vesicles protein tracers fill channels of smooth ER or Golgi complex and multivesicular bodies illustrating a process probably involved in metabolic or secretory processes.
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Akmayev IG, Fidelina OV, Kabolova ZA, Popov AP, Schitkova TA (1973) Morphological aspects of the hypothalamo-hypophyseal system. IV. Medial basal hypothalamus. An experimental morphological study. Z Zellforsch 137:493–512
Ben-Jonathan N, Mical RS, Porter JC (1974) Transport of LRF from CSF to hypophysial portal and systemic blood and release of LH. Endocrinology 95:18–25
Coates PW, Davis SL (1977) The sheep third ventricle: scanning electron microscopy of estrous anestrous and estrogen — progesterone treated anestrous ewes. Biol Reprod 17:567–573
Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–302
Gröschel-Stewart U, Unsicker K, Leonhardt H (1977) Immunohistochemical demonstration of contractile proteins in astrocytes, marginal glial and ependymal cells in the rat diencephalon. Cell Tissue Res 180:133–137
Heller A, Hutchens JO, Kirby ML, Karapas F, Fernandez C (1979) Stereotaxic electrode placement in the neonatal rat. J Neurosci Methods I:41–76
Jost S (1974) Endocrinology of sex and the development of normal organism. In: Astaurov BL (eds) Hormonal factors of individual development. Publ House “Nauka”, Moscow, pp 19–29
Kobayashi H (1975) Absorption of cerebrospinal fluid by ependymal cells of the median eminence. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system. Karger, Basel, pp 109–122
Kratin JG, Guselnikow VI (1971) The technique and methods of electroencephalography. Publ House “Nauka”, Leningrad (Russ).
Mikami S (1975) A correlative ultrastructural analysis of the ependymal cells of the third ventricle of Japanese quail, Coturnix coturnix japonica. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system. Karger, Basel, pp 80–93
Mitskevich MS (1974) Some aspects of the problem of hormonal control mechanisms in prenatal life. Ontogenez 5:557–567
Mitskevich MS (1977) Hormones and hormonal mechanisms establishment during the early ontogenesis. In: Kavetskii RE (ed) New ideas about the hormones and the mechanism of their action. Publ House “Naukova Dumka”, Kiev (Russ.), pp 110–113
Nakai Y, Naito N (1975) Uptake and biderectional transport of peroxidase injected into the blood and cerebrospinal fluid by ependymal cells of median eminence. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system. Karger, Basel, pp 94–108
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–213
Schachenmayr W (1967) Über die Entwicklung von Ependym and Plexus chorioideus der Ratte. Z Zellforsch 77:25–63
Scott DE, Krobisch-Dudley G (1975) Ultrastructural analysis of the mammalian median eminence. Morphological correlates of transependymal transport. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system. Karger, Basel, pp 29–39
Ugrumov MV, Chandrasekhar K, Borisova NA, Mitskevich MS (1979) Light and electron microscopical investigation on the tanycyte differentiation during the perinatal period in the rat. Cell Tissue Res 201:295–303
Valenstein T, Case B, Valenstein CB (1969) Stereotaxic atlas of the infant rat hypothalamus. Dev Psychobiol 2:75–80
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Ugrumov, M.V., Mitskevich, M.S. The adsorptive and transport capacity of tanycytes during the perinatal period of the rat. Cell Tissue Res. 211, 493–501 (1980). https://doi.org/10.1007/BF00234403
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DOI: https://doi.org/10.1007/BF00234403