Summary
Glomus cells from carotid bodies of adult rats dissociated by means of collagenase or collagenase + trypsin were used to study by electron microscopy the endocytotic uptake of cationized ferritin (CF) tracer into subcellular compartments. The glomus cells were incubated with the tracer (1) in a basic salt medium (BM), or (2) in the BM into which calcium ionophore A23187 had been added, or (3) in a potassium-rich medium.
Incubation of the cells in BM containing CF for 30 min resulted in attachment of the tracer to the cell membrane and uptake of a few solitary tracer particles into small vesicles and multivesicular bodies. No uptake into the cisternae of the Golgi apparatus was observed. Further incubation in BM containing CF for another 30 min resulted in increased uptake of the tracer into small vesicles and multivesicular bodies. A similar pattern of uptake was observed when the dissociated glomus cells were first preincubated in BM with CF for 30 min and then incubated for 1 min or 30 min in the BM solution containing both the ionophore and CF. Upon such incubation, CF particles were seen to penetrate into coated pits and sites of exocytosis at the cell surface. When the 30-min preincubation in BM was followed by incubation in a CF-containing potassium-rich medium for 15–30 min, uptake into vesicles, small lysosomes and occasionally also into profiles of the smooth endoplasmic reticulum was seen. Endocytotic mechanisms of the glomus cells are outlined.
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References
Abrahams SJ, Holzman E (1973) Secretion and endocytosis in insulin-stimulated rat adrenal medulla. J Cell Biol 56:540–558
Baker PF, Knight DE (1981) Calcium control of exocytosis and endocytosis in bovine adrenal medullary cells. Philos Trans R Soc Lond [Biol] 296:83–103
Böck P (1973) Das Glomus caroticum der Maus. Ergeh Anat Entwickl Gesch 48:1–82
Farquhar MG (1978) Recovery of surface membrane in anterior pituitary cells. Variations in traffic detected with anionic and cationic ferritin. J Cell Biol 78:R 35-R 42
Gonzalez C, Fidone S (1977) Increased release of 3H-dopamine during low O2 stimulation of rabbit carotid body in vitro. Neurosci Lett 6:95–99
Grönblad M, Åkerman KE, Eränkö O (1979) Induction of exocytosis from glomus cells by incubation of the carotid body of the rat with calcium and ionophore A23187. Anat Rec 195:387–396
Grönblad M, Åkerman KE, Eränkö O (1980) Ultrastructural evidence of exocytosis from glomus cells after incubation of adult rat carotid bodies in potassium-rich calcium-containing media. Brain Res 189:576–581
Grönblad M, Åkerman KEO, Eränkö O (1981) Electron-dense precipitates in glomus cells of rat carotid body after fixation in glutaraldehyde and pyroantimonate-osmium tetroxide mixture as possible indicators of calcium localization. Cell Tissue Res 217:93–104
Hansen JT (1977) Freeze-fracture study of the carotid body. Am J Anat 148:295–300
Hellström S, Hanbauer I (1981) Modification of the dopamine content in rat carotid body by metacholine and hypoxia. In: Belmonte C, Pallot DJ, Acker H, Fidone S (eds) Arterial Chemoreceptors. Leicester University Press, Leicester, pp 220–231
Hellström S, Hanbauer I, Costa E (1976) Selective decrease of dopamine content in rat carotid body during exposure to hypoxic conditions. Brain Res 118:352–355
Herzog V, Miller F (1978) Membrane retrieval in secretory cells. Secretory Mechanisms-Joint Symposium by the British Society for Cell Biology and the Society for Experimental Biology, September 1978, Guilford, England
Herzog V, Miller F (1979) Route of luminal plasma membrane retrieved by endocytosis in thyroid follicle cells. Biol Cellulaire 36:163–166
Heuser JE, Reese TS, Dennis MJ, Jan Y, Jan L, Evans L (1979) Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release. J Cell Biol 81:275–300
Holzman E, Mercurio AM (1980) Membrane circulation in neurons and photoreceptors: some unresolved issues. Int Rev Cytol 67:1–67
Kondo H (1981) Evidences for the secretion of chief cells in the rat carotid body. In: Belmonte C, Pallet DJ, Acker H, Fidone S (eds) Arterial chemoreceptors. Leicester University Press, Leicester, pp 45–54
McDonald DM (1976) Structure and function of reciprocal synapses in interconnecting glomus cells and sensory nerve terminals. In: Coupland RE, Fujita T (eds) Chromaffin, enterochromaffin and related cells. Elsevier, Amsterdam Oxford New York, pp 375–394
McDonald DM (1980) Regulation of chemoreceptor sensitivity in the carotid body: the role of presynaptic sensory nerves. Fed Proc 39:2627–2635
Nagasawa J, Douglas WW (1972) Thorium dioxide uptake into adrenal medullary cells and the problem of recapture of granule membrane following exocytosis. Brain Res 37:141–146
Ottosen PD, Courtoy PJ, Farquhar MG (1980) Pathways followed by membrane recovered from the surface of plasma cells and myeloma cells. J Exp Med 152:1–19
Pelletier G (1973) Secretion and uptake of peroxidase by rat adenohypophyseal cells. J Ultrastruct Res 43:445–459
Plattner H (1981) Membrane behaviour during exocytosis. Cell Biol Int Reports 5:435–459
Smith JE, Reese TS (1980) Use of aldehyde fixatives to determine the rate of synaptic transmitter release. J Exp Biol 89:19–29
Suchard SJ, Corcoran JJ, Pressman BC, Rubin RW (1981) Evidence for secretory granule membrane recycling in cultured adrenal chromaffin cells. Cell Biol Int Reports 5:953–962
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Grönblad, M., Åkerman, K.E.O. & Eränkö, O. Endocytotic uptake of cationized ferritin tracer into glomus cells dissociated from the adult rat carotid body. Cell Tissue Res. 226, 37–49 (1982). https://doi.org/10.1007/BF00217080
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DOI: https://doi.org/10.1007/BF00217080