Summary
In a previous study changes in the macrodistribution of fibronectin during rat-lung development were examined. Using the peroxidase-antiperoxidase immunocytochemical technique, we have demonstrated the presence of fibronectin in embryonic, neonatal, and adult rat lung at the ultrastructural level. In the embryo, fibronectin is found both in an intra-and extracellular association with isolated pneumoblasts, and in a periodic distribution along the basal lamina. The neonate displays fibronectin in an intracellular association with early type-I cells and on their basal and luminal surfaces, but not in association with type-II cells. Neonatal basal lamina is diffusely labeled by anti-fibronectin antiserum. Fibronectin in adult tissue is found both intracellularly and on the basal and luminal surfaces of type-I cells but not in type-II cells. The basal lamina and interstitial connective tissue are slightly or non-reactive. These observations confirm and extend our initial suggestion that fibronectin is involved in rat-lung development.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adamson IVR, Bowden DH (1975) Derivation of type I epithelium from type II cells in the developing rat lung. Lab Invest 32:736–745
Alescio T (1973) Effect of a proline analogue, azetidine-2-carboxylic acid, on the morphogenesis in vitro of mouse embryonic lung. J Embryol Exp Morphol 29:439–443
Alitalo K, Hovi T, Vaheri A (1980) Fibronectin is produced by human macrophages. J Exp Med 151:602–613
Beachey EH, Simpson WA (1982) The adherence of group-A streptococci to otopharyngeal cell—the lipoteicoic acid adhesion and fibronectin receptor. Infection 10:107–111
Chen LB, Murray A, Segal RA, Bushnell A, Walsh ML (1978) Studies on intercellular LETS Glycoprotein matrices. Cell 14:377–391
Courtoy PJ, Kanwar YS, Hynes RO, Farquhar MG (1980) Fibronectin localization in the rat glomerulus. J Cell Biol 87:691–696
Der CJ, Stainbridge EJ (1980) Alterations in the extracellular matrix organization associated with the reexpression of tumorigenicity in human cell hybrids. Int J Cancer 26:451–459
Eldred WD, Zucker C, Karten HS, Yazulla S (1982) Comparison of several fixation and penetration enhancement procedures for use in ultrastructural immunocytochemistry. J Histochem Cytochem (In press)
Greenberg JH, Seppa S, Seppa H, Tyl-Hewitt A (1981) Role of collagen and fibronectin in neural crest cell adhesion and migration. Dev Biol 87:259–266
Hirai KT (1968) Specific affinity of oxidized amine dye (radical intermediate) for heme enzymes: Study in light and electron microscopy and spectrophotometrically. Acta Histochem Cytochem 1:54–68
Kurkinen M, Vaheri A, Roberts PJ, Stenman S (1980) Sequential appearance of fibronectin and collagen in experimental granulation tissue. Lab Invest 43:47–51
Ledger PW, Uchida N, Tanzer ML (1980) Immunocytochemical localization of procollagen and fibronectin in human fibroblasts: effects of the monovalent ionophore, Monensin. J Cell Biol 87:663–671
McAuslan BR, Gole GA (1980) Cellular and molecular mechanisms in angiogenesis. Trans Ophthalmol Soc UK 100:354–358
Newman SA, Frisch HL (1979) Dynamics of skeletal pattern formation in developing chick limb. Science 205:6628
O'Hare KH, Sheridan MN (1970) Electron microscopic observations on the morphogenesis of the albino rat lung, with special reference to pulmonary epithelial cells. Am J Anat 127:181–205
Podleski TR, Greenberg I, Schlessinger J, Yamada KM (1979) Fibronectin delays the fusion of L6 myoblasts. Exp Cell Res 122:317–326
Rosenkrans WA, Albright JR, Hausman RE, Penney DP (1983) Light microscopic immunocytochemical localization of fibrorectin in the developing rat lung. Cell Tissue Res (in press)
Schneeberger EE (1976) The ultrastructural basis for alveolar-capillary permeability to protein. In: Lung liquids, Ciba Foundation Symposium No 38, Elsevier Amsterdam, p 3
Simpson WA, Hasty DL, Mason JM, Beachey EH (1982) Fibronectin-mediated binding of group-A streptococci to human polymorphonuclear leukocytes. Infect Immun 37:805–810
Villager B, Kelley DG, Engleman W, Kuhn C, McDonald JA (1981) Human alveolar macrophage fibronectin: synthesis, secretion, and ultrastructural localization during gelatin coated latex particle binding. J Cell Biol 90:711–720
Woods DE, Bass JA, Johanson WG Jr, Straus DC (1980) Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients. Infect Immun 30:694–699
Yamada KM (1982) Fibronectin and other structural proteins. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press, New York, pp 95–110
Yamada KM, Olden K (1978) Fibronectins: adhesive glycoproteins of the cell surface and blood. Nature 275:179–184
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rosenkrans, W.A., Albright, J.T., Hausman, R.E. et al. Ultrastructural immunocytochemical localization of fibronectin in the developing rat lung. Cell Tissue Res. 234, 165–177 (1983). https://doi.org/10.1007/BF00217410
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00217410