Summary
Administration of Concanavalin A (Con A) to cultured skin fibroblasts derived from chick embryos at two developmental stages produce variations in the relative concentration of individual glycosaminoglycan (GAG) secreted by the cells. This effect is different: at 7 days (increase of hyaluronic acid and dermatan sulphate and decrease of chondroitin sulphate) and at 14 days (dermatan sulphate is not detectable).
All the cells bind the Con A specifically, but a different pattern of agglutination is present in fibroblasts of the two embryonic ages. Since Con A is well known to bind carbohydrate-containing surface proteins, the result suggests that the release of GAG by chick embryonic fibroblasts can be modulated by cell surface receptors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bernfield MR, Cohn RH, Banerjee (1973) Glycosaminoglycans and epithelial organ formation. Am Zool 13:1067–1083
Breen M, Wenstein HG, Andersen L, Veis A (1970) Acidic glycosaminoglycans in human skin during fetal development and adult life. Anal Biochem 35:146–159
Brown JC, Hunt RC (1978) Lectins. Int Rev Cytol 52:277–343
Carinci P, Simonelli L, Bubola G, Pettazzoni P (1976) An ultrastructural analysis of chick embryonic skin development in vitro. J Exp Zool 197:349–402
Caruso A, Stabellini G, Evangelisti R, Becchetti E, Bodo MA (1983) Cell surface heterogeneity in chick embryo fibroblast population grown in vitro. IRCS Med Sci 11:336
Conrad GW, Hamilton C, Haynes E (1977) Differences in glycosa minoglycans synthesized by fibroblast-like cells from chick cornea, heart and skin. J Biol Chem 252:6861–6870
Dodson JW (1967) The differentiation of epidermis. I. The interrelationship of epidermis and dermis in embryonic chicken skin. J Embryol Exp Morphol 17:83–105
Engfelt B, Hjerpe A (1976) Glycosaminoglycans of cartilage and bone tissue in two cases of osteogenesis imperfecta congenita. Acta Pathol Microbiol Scand 84:488–494
Evangelisti R, Caruso A, Stabellini G (1977) Electrophoretic identification of glycosaminoglycans in avian rudiments. Uppsala J Med Sci 82:129
Evangelisti R, Stabellini G, Venturoli A, Carinci P (1982) Glycosaminoglycan synthesis by embryonic fibroblasts is agedependent and modulated by environmental factors. Experientia 38:616–617
Fleischmajer R, Perlish JS, Bashey RI (1972) Human dermal glycosaminoglycans and aging. Biochim Biophys Acta 279:265–275
Fukui S, Yoshida H, Tanaka T, Sakano T, Usui T, Yamashima I (1981) Glycosaminoglycan synthesis by cultured skin fibroblasts from a patient with Lowe's syndrome. J Biol Chem 256:10313–10318
Gressner AM, Paren H, Greiling H (1977) The synthesis of total and specific glycosaminoglycan during development of experimental liver cirrhosis. Experientia 33:1290–1292
Hakomori S (1975) Structure and organization of cell surface glycolipids and glycoproteins, dependency on cell growth and malignant transformation. Biochim Biophys Acta 417:55–89
Hart GW (1976) Biosynthesis of glycosaminoglycans during corneal development. J Biol Chem 251:6513–6521
Hata R, Nagai Y (1972) A rapid and micro method for separation of acidic glycosaminoglycans by two-dimensional electrophoresis. Anal Biochem 45:462–468
Hata R, Nagai Y (1973a) Distribution of acidic glycosaminoglycans in tadpole back skin. Biochim Biophys Acta 304:408–412
Hata R, Nagai Y (1973b) A micro colorimetric determination of acidic glycosaminoglycans by two dimensional electrophoresis on a cellular acetate strip. Anal Biochem 52:652–656
Horwitz AL, Cristal RG (1975) Content and synthesis of glycosaminoglycans in the developing lung. J Clin Invest 56:1312–1318
Kapoor R, Bourier S, Prehm P (1983) Glycosaminoglycan synthesis in skin fibroblasts from patients with osteogenesis imperfecta. FEBS Lett 152:183–186
Karlinsky JB (1982) Glycosaminoglycan in emphysematous and fibrotic hamster lung. Am Rev Respir Dis 125:85–88
Kleinschuster SJ, Moscona AA (1972) Interactions of embryonic and fetal neural retina cells with carbohydrate-binding phytoagglutinins: cell surface changes with differentiation. Exp Cell Res 70:397–410
Kurtz MJ, Stidworthy GH (1975) Control over acid mucopolysacaride production of cells in vitro. Biochim Biophys Acta 399:90–100
Metalon R, Dorman A (1968) The structure of acidic mucopolysaccaride produced by Hurler fibroblasts in tissue culture. Proc Natl Acad Sci USA 60:179–185
Nicolson GL (1974) The interactions of lectins with animal cell surface. Int Rev Cytol 39:89–190
Patterson Jr MK (1979) In: WB Jacoby IH, IH Pastan (eds) Methods in enzymology. Academic Press, New York, 58:141–152
Saarni H (1978) Cortisol effect on the glycosaminoglycan sinthesis and molecular weight distribution in vitro. Biochem Pharmacol 27:1029–1032
Saarni H, Tammi M (1978) Time and concentration dependence of the action of cortisol on fibroblasts in vitro. Biochim Biophys Acta 540:117–126
Schmid K, Jusco JG, Kimura A, Tschopp FA, Zollinger R, Binette JP, Lewis W, Hayashi S (1982) The distribution of the glycosaminoglycans in anatomic components of the lung and the changes concentration of these macromolecules during development and aging. Biochim Biophys Acta 716:178–187
Tomida M, Koyama H, Ono T (1977) A serum factor capable of stimulating hyaluronic acid synthesis in cultured rat fibroblasts. J Cell Physiol 91:323–328
Toole BP, Trelstad RL (1971) Hyaluronate production and removal during corneal development in chick. Dev Biol 26:28–35
Venray M, Aubery M (1982) Differential effect of tunicamycin on glycosylation of cell surface glycoproteins from chick fibroblasts during embryo development. CR Seances Acad Sci [III] 234:371–374
Warren L, Fuhrer JP, Buck CA (1973) Surface glycoprotein of cells before and after transformation by oncogenic viruses. Fed Proc 32:80–85
Wever J, Schachtschabel DD, Sluke G, Wever G (1980) Effect of short or long term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblast in culture. Mech Ageing Dev 14:89–99
Zalik SE, Cook GMW (1976) Comparison of early embryonic and differentiating cell surfaces Interaction of lectins with plasma membrane components. Biochim Biophys Acta 419:119–136
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evangelisti, R., Bodo, M., Caruso, A. et al. Extracellular glycosaminoglycans (GAG) released by chick embryonic fibroblasts. Cell Tissue Res. 238, 241–245 (1984). https://doi.org/10.1007/BF00217295
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00217295