Summary
Mitochondria isolated from heart tissue after a 1-min perfusion with Hanks medium were found to have significantly lower rates of State-3 respiration and respiratory control ratios compared to mitochondria isolated from non-perfused hearts. Examination of the mitochondrial preparations by electron microscopy revealed that a large proportion of the mitochondria isolated from perfused heart tissue were swollen and broken compared to mitochondria from non-perfused hearts.
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Staecker, J.L., Nadakavukaren, M.J. & Richardson, A. Effect of brief vascular perfusion on the ultrastructure and activity of mitochondria isolated from the rat heart. Cell Tissue Res. 240, 247–249 (1985). https://doi.org/10.1007/BF00217581
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DOI: https://doi.org/10.1007/BF00217581