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Multigranular exocytosis induced by phospholipase A2-activators, melittin and mastoparan, in rat anterior pituitary cells

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Summary

Effects of phospholipase A2-activators, melittin and mastoparan, on rat anterior pituitary cells were studied by use of the electron microscope. Rat anterior pituitaries were incubated in HEPES buffer containing 20 μg/ml of melittin or the same dose of mastoparan for 5 min, 10 min and 20 min. Features indicating discharge of granule contents by exocytosis were increased with time, and the simultaneous extrusion of a number of secretory granules, named “multigranular exocytosis”, was often recognized in addition to single-granule exocytosis at 10 min and 20 min. Most membrane pits, where the multigranular exocytosis as well as the single-granule exocytosis occurred, were coated. Moreover, a large number of vesicles coated or noncoated were distributed near the trans side of the Golgi apparatus of melittin-treated or mastoparan-treated cells after 20 min. These vesicles might be related to membrane internalized from the excess surface membrane derived from the limiting membrane of exocytosed granules. These observations indicate that phospholipase A2-activators induce hormone release involving membrane fusion between limiting membranes of secretory granules, and between granulelimiting membrane and plasma membrane in rat anterior pituitary cells.

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Authors and Affiliations

  1. Department of Anatomy, Osaka University Medical School, Osaka, Japan

    Hidetake Kurihara, Koichi Kitajima, Takao Senda &  Hisao Fujita

  2. Faculty of Pharmaceutical Science, University of Tokyo, Hongo, Tokyo, Japan

    Terumi Nakajima

Authors
  1. Hidetake Kurihara
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  2. Koichi Kitajima
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  3. Takao Senda
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  4. Hisao Fujita
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  5. Terumi Nakajima
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This study was supported by grants from the Japan Ministry of Education

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Kurihara, H., Kitajima, K., Senda, T. et al. Multigranular exocytosis induced by phospholipase A2-activators, melittin and mastoparan, in rat anterior pituitary cells. Cell Tissue Res. 243, 311–316 (1986). https://doi.org/10.1007/BF00251045

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  • DOI: https://doi.org/10.1007/BF00251045

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