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Lymphocytes of the intestinal mucosa of chickens

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Summary

Examination, by light and electron microscopy, of the morphology and the staining properties of intraepithelial lymphocytes from the intestine of the chicken revealed a population of lymphoid cells, of which a proportion (up to 20%) is granulated. The majority of cells were immunoreactive with anti-T cell serum and can therefore be considered to be related to T-lymphocytes, but they did not proliferate when cultured with phytohaemagglutinin. The granulated cells were identical to those previously designated globule-containing leukocytes, but were distinct from mast cells in their morphology, staining reactions and the stability of the granules in different fixatives and buffers.

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References

  • Arnaud-Battandier F, Lawrence EC, Blaese RM (1980) Lymphoid populations of gut mucosa in chickens. Dig Dis Sci 25:252–259

    PubMed  Google Scholar 

  • Askenase PW (1980) Itnmunopathology of parasitic diseases: involvement of basophils and mast cells. Springer Semin Immunopathol 2:417–442

    Google Scholar 

  • Bäck O (1970a) Studies on the lymphocytes in the intestinal epithelium of the chicken. 3. Effect of thymectomy. Int Arch Allergy Appl Immunol 39:192–200

    PubMed  Google Scholar 

  • Bäck O (1970b) Studies on the lymphocytes in the intestinal epithelium of the chicken. 4. Effect of bursectomy. Int Arch Allergy Appl Immunol 39:342–351

    PubMed  Google Scholar 

  • Bäck O (1972a) Studies on the lymphocytes in the intestinal epithelium of the chicken. 1. Ontogeny. Acta Pathol Microbiol Scand Section A 80:84–90

    Google Scholar 

  • Bäck O (1972b) Studies on the lymphocytes in the intestinal epithelium of the chicken. 2. Kinetics. Acta Pathol Microbiol Scand Section A 80:91–96

    Google Scholar 

  • Befus DJ, Bienenstock J (1982) Factors involved in symbiosis and host resistance at the mucosa-parasite interface. Progr Allergy 31:76–177

    Google Scholar 

  • Bjerregaard P (1975) Lymphoid cells in chicken intestinal epithelium. Cell Tissue Res 161:485–495

    PubMed  Google Scholar 

  • Burnet FM (1977) The probable relationship of some or all mast cells to the T-cell system. Cell Immunol 30:358–360

    PubMed  Google Scholar 

  • Collan Y (1972) Characteristics of non-epithelial cells in the epithelium of normal rat ileum: a light and electron microscopical study. Scand J Gastroenterol [Suppl] 7:18, 1–66

    Google Scholar 

  • Daimon T, Caxton-Martins A (1977) Electron microscopic and enzyme cytochemical studies on granules of mature chicken granular leukocytes. J Anat 123:553–562

    PubMed  Google Scholar 

  • Davies MDJ, Parrott DMV (1981) The preparation and purification of lymphocytes from the epithelium and lamina propria of murine small intestine. Gut 22:481–488

    PubMed  Google Scholar 

  • Ferguson A (1977) Intraepithelial lymphocytes of the small intestine. Gut 18:921–937

    PubMed  Google Scholar 

  • Goodacre R, Davidson R, Singal D, Bienenstock J (1979) Morphological and functional characteristics of human intestinal lymphoid cells isolated by a mechanical technique. Gastroenterology 76:300–308

    PubMed  Google Scholar 

  • Gregory MW (1979) The globule leukocyte and parasitic infection — a brief history. Vet Bull 49:821–827

    Google Scholar 

  • Guy-Grand D, Griscelli C, Vassalli P (1978) The mouse gut T-lymphocyte, a novel type of T-cell: nature, origin and traffic in mice in normal and graft-versus-host conditions. J Exp Med 148:1146–1160

    PubMed  Google Scholar 

  • Keasbey LE (1923) On a new form of leucocyte (Schollenleukozyt, Weill) as found in the gastric mucosa of sheep. Folia Haematol Lpz 29:155–171

    Google Scholar 

  • Kitagawa M, Ogata K, Sugimura M, Hashimoto Y, Kudo N (1979) Thymus origin of globule leucocytes in chicken. Jpn J Vet Res 27:16–18

    PubMed  Google Scholar 

  • Lawn AM, Rose ME (1982) Mucosal transport of Eimeria tenella in the cecum of the chicken. J Parasitol 68:1117–1123

    PubMed  Google Scholar 

  • Mayrhofer G (1980) Fixation and staining of granules in mucosal mast cells and intraepithelial lymphocytes in the rat jejunum, with special reference to the relationship between the acid glycosaminoglycans in the two cell types. Histochem J 12:513–526

    PubMed  Google Scholar 

  • Millard BJ, Lawn AM (1982) Parasite-host relationships during the development of Eimeria dispersa Tyzzer, 1929, in the turkey (Meleagris gallopavo gallopavo) with a description of intestinal intra-epithelial leucocytes. Parasitology 84:13–20

    PubMed  Google Scholar 

  • Miller HRP (1980) The structure, origin and function of mucosal mast cells. A brief review. Biol Cell 39:229–232

    Google Scholar 

  • Payne LN, Powell PC, Rennie M (1974) Response of B and T lymphocytes and other blood leukocytes in chickens with Marek's disease. Cold Spring Harbor Symp Quant Biol 34:817–826

    Google Scholar 

  • Pearce FL (1983) Mast cell heterogeneity. TIPS

  • Powell PC (1980) The influence of erythrocytes on the stimulation of chicken lymphocytes by phytohaemagglutinin. Av Pathol 9:465–470

    Google Scholar 

  • Rose ME, Hesketh P (1982) Immunity to coccidia in chickens: adoptive transfer with peripheral blood lymphocytes and spleen cells. Parasite Immunol 54:171–185

    Google Scholar 

  • Rose ME, Hesketh P (1984) Infection with Eimeria tenella: modulation of lymphocyte blastogenesis by specific antigen, and evidence for immunosuppression. J Protozool 31:549–553

    PubMed  Google Scholar 

  • Rose ME, Ogilvie BM, Bradley JWA (1980) Intestinal mast cell response in rats and chickens to coccidiosis, with some properties of chicken mast cells. Int Arch Allergy Appl Immunol 63:21–29

    PubMed  Google Scholar 

  • Rose ME, Lawn AM, Millard BJ (1984) The effect of immunity on the early events in the life cycle of Eimeria tenella in the caecal mucosa of the chicken. Parasitology 88:199–210

    PubMed  Google Scholar 

  • Schrader JW, Scollay R, Battye F (1983) Intramucosal lymphocytes of the gut: Lyt-2 and Thy-1 phenotype of the granulated cells and evidence for the presence of both T-cells and mast cell precursors. J Immunol 130:558–564

    PubMed  Google Scholar 

  • Selby WS, Janossy G, Goldstein G, Jewell DP (1981) T-lymphocyte subsets in human intestinal mucosa: the distribution and relationship to MHC-derived antigens. Clin Exp Immunol 44:453–458

    PubMed  Google Scholar 

  • Singal DP, O'Neill M, Clancy R, Bienenstock J (1976) Functional T-cells in rabbits gut mucosal lymphocytes. Gut 17:325–327

    PubMed  Google Scholar 

  • Tagliabue A, Befus AD, Clark DA, Bienenstock J (1982) Characteristics of natural killer cells in the murine intestinal epithelium and lamina propria. J Exp Med 155:1785–1796

    PubMed  Google Scholar 

  • Tagliabue A, Nencioni L, Villa L, Keren DF, Lowell GH, Boraschi D (1983) Antibody-dependent cell-mediated antibacterial activity of intestinal lymphocytes with secretory IgA. Nature 306:184–186

    PubMed  Google Scholar 

  • Toner PG (1965) The fine structure of the globule leucocyte in the fowl intestine. Acta Anat 61:321–330

    PubMed  Google Scholar 

  • Weill P (1919) Über die leukocytären Elemente der Darmschleimhaut der Säugetiere. Ein Beitrag zur Beurteilung der Granulationen in Leukocyten. Arch Mikrosk Anat 93:1–81

    Google Scholar 

  • Wight PAL, Mackenzie GM (1970a) The mast cells of Gallus domesticus. I. Distribution and ultrastructure. Acta Anat 75:100–113

    PubMed  Google Scholar 

  • Wight PAL, Mackenzie GM (1970b) The mast cells of Gallus domesticus. II. Histochemistry. Acta Anat 75:263–275

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Houghton Poultry Research Station, Houghton, Huntingdon, Cambridgeshire, UK

    A. M. Lawn, M. E. Rose, J. W. A. Bradley & M. C. Rennie

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  1. A. M. Lawn
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  2. M. E. Rose
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  3. J. W. A. Bradley
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  4. M. C. Rennie
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Lawn, A.M., Rose, M.E., Bradley, J.W.A. et al. Lymphocytes of the intestinal mucosa of chickens. Cell Tissue Res. 251, 189–195 (1988). https://doi.org/10.1007/BF00215464

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  • DOI: https://doi.org/10.1007/BF00215464

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