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An immunohistochemical study on the postnatal development of rat nasal-associated lymphoid tissue (NALT)

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Summary

This study concerns the development of nasal-associated lymphoid tissue in the rat, using immuno- and enzyme-histochemical staining techniques on cryostat sections. Nasal-associated lymphoid tissue is present at birth as a small accumulation of mainly T lymphocytes and non-lymphoid cells; B cells are rare. Distinct areas of T and B cells appear at 10 days after birth; by that time high endothelial venules are also observed. Intra-epithelial lymphocytes are present, most of them being T-helper cells. ED1+ macrophages are seen throughout the tissue. The proportion of ED1+cells does not change during ontogeny. ED2+cells (tissue macrophages) are present predominantly at the border between the lymphoid tissue and the surrounding connective tissue, in all age-groups. ED3+mononuclear cells are scattered throughout the nasal-associated lymphoid tissue of young animals. Later on, the ED3+ cells migrate into the border-area between lymphoid and connective tissue. Ia+ non-lymphoid cells in the nasal lymphoid tissue increase in number during ontogeny. Only a few of them show acid phosphatase activity, indicating that the proportion of classical scavenger macrophages is low. Some of them may be antigen presenting (dendritic) cells. Ia+ dendritic cells also occur between the epithelial cells. Moreover, some epithelial cells express the Ia marker.

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Authors and Affiliations

  1. Department of Histology, Medical Faculty, Vrije Universiteit, Amsterdam, The Netherlands

    Dona M. H. Hameleers, Marja van der Ende, Jeike Biewenga & Taede Sminia

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  1. Dona M. H. Hameleers
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  2. Marja van der Ende
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  3. Jeike Biewenga
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  4. Taede Sminia
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Hameleers, D.M.H., van der Ende, M., Biewenga, J. et al. An immunohistochemical study on the postnatal development of rat nasal-associated lymphoid tissue (NALT). Cell Tissue Res. 256, 431–438 (1989). https://doi.org/10.1007/BF00218901

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