Summary
Electron microscopy of cat parotid glands revealed great heterogeneity in the secretory granules of normal unstimulated acinar cells. Electrical stimulation of the parasympathetic nerve to the gland evoked a copious flow of parotid saliva which was accompanied by an extensive depletion of the secretory granules from the acinar cells. Exocytosis was captured as it was occurring by means of perfusion-fixation, and showed that the events occur in a conventional manner. Stimulation of the sympathetic nerve caused only a very small flow of saliva, and no acinar degranulation was detected. It can be concluded that the parasympathetic secretomotor axons provide the main drive for parotid acinar degranulation in the cat. This contrasts with the rat in which sympathetic impulses provide the main stimulus for parotid acinar degranulation. These dissimilarities serve to emphasise how extensively species differences may influence autonomic responses in salivary glands.
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References
Burgen ASV (1964) Techniques for stimulating the auriculo-temporal nerve and recording the flow of saliva. In: Sreebny LM, Meyer J (eds) Salivary glands and their secretion. Pergamon, Oxford, pp 303–307
Burgen ASV, Emmelin NG (1961) Physiology of the salivary glands. Edward Arnold, London
Emmelin N (1987) Nerve interactions in salivary glands. J Dent Res 66:509–517
Emmelin N, Garrett JR (1986) Secretory changes in parotid acini of cats on nerve stimulation. J Physiol 376:29P
Emmelin N, Grampp W, Thesleff P (1980) Sympathetically evoked secretory potentials in the parotid gland of the cat. J Physiol 302:183–195
Garrett JR (1972) Neuro-effector sites in salivary glands. In: Emmelin N, Zotterman Y (eds) Oral Physiology. Pergamon, Oxford, pp 83–97
Garrett JR, Kidd A (1975) Effects of nerve stimulation and denervation on secretory material in submandibular striated duct cells of cats, and the possible role of these cells in the secretion of salivary kallikrein. Cell Tissue Res 161:71–84
Garrett JR, Kidd A (1976) Acid phosphatase and peroxidase in “resting” acinar cells of the major salivary glands of cats and their possible movement into secretory granules. Histochem J 8:523–538
Garrett JR, Thulin A (1975) Changes in parotid acinar cells accompanying salivary secretion in rats on sympathetic or parasympathetic nerve stimulation. Cell Tissue Res 159:179–193
Harrison JD (1974) Salivary glands of the cat: a histochemical study. Histochem J 6:649–664
Harrop TJ, Garrett JR (1974) Effects of preganglionic sympathectomy on secretory changes in parotid acinar cells of rats on eating. Cell Tissue Res 154:135–150
Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137–138A
Kyriacou K, Garrett JR, Gjörstrup P (1988) Structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands. Arch Oral Biol 33:271–280
Nagato T, Tandler B (1986) Ultrastructure of dog parotid gland. J Submicrosc Cytol 18:67–74
Palade G (1975) Intracellular aspects of the process of protein synthesis. Science 189:347–358
Schneyer CA (1974) Autonomic regulation of secretory activity and growth responses of rat parotid gland. In: Thorn NA, Petersen OH (eds) Secretory mechanisms of exocrine glands. Munksgaard, Copenhagen, pp 42–55
Wallach D (1982) The Secretory granule of the parotid gland. In: Poisner AM, Trifaro JM (eds) The secretory granule. The secretory process, Vol 1. Elsevier, Amsterdam, pp 247–276
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Emmelin, N., Garrett, J.R. Nerve-induced secretion of parotid acinar granules in cats. Cell Tissue Res. 257, 549–554 (1989). https://doi.org/10.1007/BF00221465
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DOI: https://doi.org/10.1007/BF00221465