Summary
The present study examined the presence and cellular distribution of angiotensinogen, the precursor to the angiotensin peptides, in the ovary of the normal cycling rat by immunocytochemistry. Angiotensinogen staining was present in the granulosa cells of maturing follicles and to a lesser extent in those undergoing atresia. Staining was not seen in the granulosa cells of primordial or early primary follicles. In maturing follicles intense staining for angiotensinogen was confined to the antral cell layers, cells of the cumulus oophorus and in the follicular fluid. Strong immunostaining was also seen in the germinal epithelium covering the ovary. Lighter angiotensinogen staining was observed in some parts of the cortical and medullary stroma and occasionally in corpora lutea. No variation in the intensity or pattern of angiotensinogen staining was observed throughout the estrous cycle. Comparison of the distribution of angiotensinogen with the previously described localization of renin, AII, angiotensin converting enzyme and AII receptors, suggests that there are a number of intra-ovarian sites at which AII could be produced.
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Culler MD, Tarlatzis BC, Lightman A, Fernandez LA, DeCherney AH, Negro-Vilar A, Naftolin F (1986) Angiotensin II-like immunoreactivity in human ovarian follicular fluid. J Clin Endocrinol Metab 63:613–615
Daud AI, Bumpus FM, Husain A (1988) Evidence for selective expression of angiotensin II receptors on atretic follicles in the rat ovary: An autoradiographic study. Endocrinology 122:2727–2734
Do YS, Sherrod A, Lobo RA, Paulson RJ, Shinagawa T, Chen S, Kjos S, Hsueh WA (1988) Human ovarian theca cells are a source of renin. Proc Natl Acad Sci USA 85:1957–1961
Fernandez LA, Tarlatzis BC, Rzasa PJ Caride VJ, Laufer N, Negro-Vilar AF, DeCherney AH, Naftolin F (1985) Renin-like activity in ovarian follicular fluid. Fertil Steril 44:219–223
Glorioso N, Atlas SA, Laragh JH, Jewelewicz R, Sealey JE (1986) Prorenin in high concentrations in human ovarian follicular fluid. Science 233:1422–1424
Howard RB, Pucell AG, Bumpus FM, Husain A (1988) Rat ovarian renin: Characterization and changes during the estrous cycle. Endocrinology 123:2331–2340
Hsu S, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabelled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Husain A, Bumpus FM, De Silva P, Speth RC (1987) Localization of angiotensin II receptors in ovarian follicles and the identification of angiotensin II in rat ovaries. Proc Natl Acad Sci USA 84:2489–2493
Kim S, Shinjo M, Fukamizu A, Miyazaki H, Usuki S, Murakami K (1987) Identification of renin and renin messenger RNA sequence in rat ovary and uterus. Biochem Biophys Res Commun 142:169–175
Krakoff LR, Eisenfeld AJ (1977) Hormonal control of plasma renin substrate (angiotensinogen). Circ Res 41 (Suppl II):43–46
Lightman A, Tarlatzis BC, Rzasa PJ, Culler MD, Caride VJ, Negro-Vilar AF, Lennard D, DeCherney AH, Naftolin F (1987a) The ovarian renin-angiotensin system: Renin-like activity and angiotensin II/III immunoreactivity in gonadotropin-stimulated and unstimulated human follicular fluid. Am J Obstet Gynecol 156:808–816
Lightman A, Deshepper CF, Mellon SH, Ganong WF, Naftolin F (1987b) In situ hybridization identifies renin mRNA in the rat corpus luteum. Gynecol Endocrinol 1:227
Lightman A, Jones CL, MacLusky NJ, Palumbo A, DeCherney AH, Naftolin F (1988) Immunocytochemical localization of angiotensin II immunoreactivity and demonstration of angiotensin II binding in the rat ovary. Am J Obstet Gynecol 159:526–530
Ohkubo H, Nakayama K, Tanaka T, Nakanishi S (1986) Tissue distribution of rat angiotensinogen mRNA and structural analysis of its heterogeneity. J Biol Chem 261:319–323
Palumbo A, Jones C, Lightman A, Carcangiu ML, DeCherney AH, Naftolin F (1989) Immunohistochemical localization of renin and angiotensin II in human ovaries. Am J Obstet Gynecol 160:8–14
Pellicer A, Palumbo A, DeCherney AH, Naftolin F (1988) Blockage of ovulation by an angiotensin antagonist. Science 240:1660–1661
Pucell AG, Bumpus FM, Husain A (1988) Regulation and angiotensin II receptors in cultured rat ovarian granulosa cells by follicle-stimulating hormones and angiotensin II. J Biol Chem 263:11954–11961
Schwartz NB, Bartosik D (1962) Changes in pituitary LH content during the rat estrous cycle. Endocrinology 71:756–762
Sernia C, Davis M, Thomas WG (1986) Purification of rat angiotensinogen. Prep Biochem 16:45–59
Speth RC, Bumpus FM, Husain A (1986) Identification of angiotensin II receptors in the rat ovary. Eur J Pharmacol 130:351–352
Speth RC, Husain A (1988) Distribution of angiotensin-converting enzyme and angiotensin II-receptor binding sites in the rat ovary. Biol Reprod 38:695–702
Thomas WG, Sernia C (1988) Immunocytochemical localization of angiotensinogen in the rat brain. Neuroscience:319–341
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Thomas, W.G., Sernia, C. The immunocytochemical localization of angiotensinogen in the rat ovary. Cell Tissue Res 261, 367–373 (1990). https://doi.org/10.1007/BF00318679
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DOI: https://doi.org/10.1007/BF00318679