Summary
The permeability of the uveoscleral outflow pathway from the anterior ocular chamber was examined in rabbit and monkey eyes using anionic ferritin as a tracer. Ferritin, infused intracamerally, had ready access to the choroidal interstitium, and the degree of penetration was generally correlated with the time and pressure relationships during infusion. In both species, there were accumulations of tracer in intercellular spaces at the lamina fusca, but tracer was also present in the sclera. Thus, in contrast to the situation in the eyes of hamsters, the uveoscleral outflow pathway in the eyes of rabbits and monkeys includes the choroidal connective tissue and allows passage of relatively large molecular weight substances.
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References
Bill A (1965) Aqueous humor drainage mechanism in the cynomolgus monkey (Macaca irus) with evidence for unconventional routes. Invest Ophthalmol 4:911–919
Bill A (1966a) Conventional and uveo-scleral drainage of aqueous humor in the cynomolgus monkey (Macaca irus) at normal and high intraocular pressures. Exp Eye Res 5:45–54
Bill A (1966b) The routes for bulk drainage of aqueous humor in rabbits with and without cyclodalysis. Doc Ophthalmol 20:157–169
Bill A (1971) Aqueous humor dynamics in monkeys (Macaca irus andCercopithecus ethiops). Exp Eye Res 11:195–206
Cole DF, Monro PAG (1976) The use of fluorescein-labelled dextrans in investigation of aqueous humor outflow in the rabbit. Exp Eye Res 23:571–585
Crawford KS, Kaufman PL (1991) Dose-related effects of prostaglandin F2 isopropylester on intracellular pressure, refraction, and pupil diameter in monkeys. Invest Ophthalmol Vis Sci 32:510–519
Gabelt BT, Kaufman PL (1989) Prostaglandin F2 α increases uveoscleral outflow in the cynomolgus monkey. Exp Eye Res 49:389–402
Gabelt BT, Kaufman PL (1990) The effect of prostaglandin F2 α on trabecular outflow facility in cynomolgus monkeys. Exp Eye Res 52:87–91
Hageman GS, Kelly DE (1984) Fibrillar and cytoskeletal substructure of tight junctions: Analysis of single-stranded tight junctions linking fibroblasts of the lamina fusca in hamster eyes. Cell Tissue Res 238:545–557
Inomata H, Bill A (1977) Exit sites of unveoscleral outflow using different-sized fluorescent tracers in normal and inflamed eyes. Exp Eye Res 45:525–532
Inomata H, Bill A, Smelser GK (1972) Aqueous humor pathways through the trabecular meshwork and into Schlemm's canal in the cynomolgus monkey (Macaca irus), and electron microscopic study. Am J Ophthalmol 73:760–789
Kanwar YS, Linker A, Farquhar MG (1980) Increased permeability of the glomerular basement membrane to ferritin after removal of glycosaminoglycans (heparan sulfate) by enzyme digestion. J Cell Biol 86:688–693
Kelly DE, Hageman GS, McGregor JA (1983) Uveal compartmentation in the hamster eye revealed by fine structural and tracer studies: Implications for unveoscleral outflow. Invest Ophthalmol Vis Sci 24:1288–1304
Knepper PA, Farbman AI, Telser AG (1981) Aqueous outflow pathway glycosaminoglycans. Exp Eye Res 32:265–277
Knepper PA, Farbman AI, Telser AG (1984) Exogenous hyaluronidases, and degradation of hyaluronic acid in the rabbit eye. Invest Ophthalmol Vis Sci 25:286–293
Koseki T, Wood RL, Kelly DE (1990) Structural analysis of potential barriers to bulk-flow exchanges between uvea and sclera in eyes of rabbits. Cell Tissue Res 259:255–263
Lutjen-Drecoll E, Tamm E (1988) Morphological study of the anterior segment of cynomolgus monkey eyes following treatment with prostaglandin F2 α. Exp Eye Res 47:761–769
McMaster RB, Macri FJ, (1968) Secondary aqueous humor outflow pathways in the rabbit, cat and monkey. Arch Ophthalmol 79:297–303
Mollenhauer HH (1964) Plastic embedding mixtures for use in electron microscopy. Stain Technol 39:111–114
Pino RM, Essner E (1980) Structure and permeability to ferritin of the choriocapillary endothelium of the rat eye. Cell Tissue Res 208:21–27
Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212
Richardson KC, Jarrett L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol 35:313–323
Richardson TM (1982) Distribution of glycosaminoglycans in the aqueous outflow system of the cat. Invest Ophthalmol Vis Sci 22:319–329
Shen J-Y, Kelly DE, Hyman S, McComb JG (1985) Intraorbital cerebrospinal fluid outflow and the posterior uveal compartment of the hamster eye. Cell Tissue Res 240:77–87
Tawara A, Varner HH, Hollyfield JG (1989) Distribution and characterization of sulfated proteoglycans in the human trabecular tissue. Invest Ophthalmol Vis Sci 30:2215–2231
Toris CB, Gregerson DS, Pedersen JE (1987) Uveoscleral outflow using different-sized fluorescent tracers in normal and inflamed eves. Exp Eye Res 45:525–532
Tripathi RC (1977) Uveoscleral drainage of aqueous humor. Exp Eye Res 25 [Suppl]:305–308
Van Buskirk EM, Brett J (1978) The canine eye: In vitro dissolution of the barriers to aqueous outflow. Invest Ophthalmol Vis Sci 17:258–263
Wood RL, Koseki T, Kelly DE (1990) Structural analysis of potential barriers to bulk-flow exchanges between uvea and sclera in eyes of macaque monkeys. Cell Tissue Res 260:459–468
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Wood, R.L., Koseki, T. & Kelly, D.E. Uveoscleral permeability to intracamerally infused ferritin in eyes of rabbits and monkeys. Cell Tissue Res. 270, 559–567 (1992). https://doi.org/10.1007/BF00645059
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DOI: https://doi.org/10.1007/BF00645059