Abstract
The present study describes substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat. About 60% of neurons in the monkey ciliary ganglion and 40% in the cat ciliary ganglion were substance P-like immunoreactive, ranging from faint to moderate staining. Substance P-like immunoreactivity was located in cell bodies, dendritic profiles and axons. In the monkey, substance P-like immunoreactive pericellular arborisations were associated with about 0.5%–3% of the ganglion cells, which were either negatively, faintly or moderately stained. An electron-microscopic study demonstrated the presence of either substance P-like immunoreactive positive or negative axon terminals synapsing or closely associated with positive dendritic profiles in both the monkey and cat ciliary ganglia. The results suggest that substance P plays an important role in the ciliary ganglion, perhaps as a modulator or transmitter.
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References
Baker SC, Cuello AC, Matthews MR (1980) Substance P-containing synapses in a sympathetic ganglion and their possible origin as collaterals from sensory nerve fibres. J Physiol 308:76–77
Bolam JP, Somogyi P, Takagi H, Fodor I, Smith AD (1983) Localization of substance P-like immunoreactivity in neurons and terminals in the neostriatum of the rat: a correlated light and electron microscopic study. J Neurocytol 12:325–344
Dryer SE, Chiapinelli VA (1985a) Substance P depolarizes nerve terminals in an autonomic ganglia. Brain Res 336:190–194
Dryer SE, Chiapinelli VA (1985b) Properties of choroid and ciliary neurons in the avian ciliary ganglion and evidence for substance P as a neurotransmitter. J Neurosci 5:2654–2661
Epstein ML, Davis JP, Gellman LE, Lamb JR, Dahl JL (1988) Cholinergic neurons of the chicken ciliary ganglion contain somatostatin. Neuroscience 25:1053–1060
Erichsen JT, Reiner A, Karten HJ (1982a) Co-occurrence of substance P-like and Leu-enkephalin-like immunoreactivities in neurons and fibres of avian nervous system. Nature 295:407–410
Erichsen JT, Karten HJ, Eldred WD, Brecha NC (1982b) Localization of substance P-like and enkephalin-like immunoreactivity within preganglionic terminal of the avian ciliary ganglion: light and electron microscopy. J Neurosci 2:994–1003
Grimes P, Sallmann L von (1960) Comparative anatomy of the ciliary nerves. Arch Ophthalmol 64:81–121
Grimes PA, Meglinn AM, Stone RA (1990) An immunohistochemically distinct population of cat ciliary ganglion cells. Brain Res 535:323–326
Hardebo JE, Suzuki N, Ekblad E, Owman C (1992) Vasoactive intestinal polypeptide and acetylcholine coexist with neuropeptide Y, dopamine-β-hydroxylase, tyrosine hydroxylase, substance P or calcitonin gene-related peptide in neuronal subpopulations in cranial parasympathetic ganglia of rat. Cell Tissue Res 267:291–300
Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975a) Substance P: localization in the central nervous system and in some primary sensory neurons. Science 190:889–890
Hökfelt T, Kellerth JO, Nilsson G, Pernow B (1975b) Experimental immunocytochemical studies on the localization and distribution of substance P in cat primary sensory neuron. Brain Res 100:235–252
Hökfelt T, Elfvin LG, Scholzverg M, Goldstein M, Nilsson G (1977) On the occurrence of substance P-containing fibres in sympathetic ganglia: immunohistochemical evidence. Brain Res 132:29–41
Katz DM, Karten HJ (1980) Substance P in the vagal sensory ganglia: localization in cell bodies and pericellular arborizations. J Comp Neurol 193:549–564
Konishi S, Okamoto T, Otsuka M (1985) Substance P as a neurotransmitter released from peripheral branches of primary afferent neurons producing slow synaptic excitation in autonomic ganglion cells. In: Jordan CC, Oehme P (eds) Substance P metabolism and biological actions. Taylor and Francis, London Philadelphia, pp 121–136
Kuntz A (1961) The autonomic nervous system. In: Hartman CG (ed) The anatomy of the rhesus monkey (Macaca mulatta). Hafner, New York, pp 328–338
Lembeck F (1985) Substance P and sensory neurons. In: Jordan CC, Oehme P (eds) Substance P metabolism and biological actions. Taylor and Francis, London Philadelphia, pp 137–151
Lindh B, Dalsgaard CK, Elfvin LG, Hökfelt T, Cuello AC (1983) Evidence of substance P immunoreactive neurons in dorsal root ganglia and vagal ganglia projecting to the guinea pig pylorus. Brain Res 269:365–369
Ling EA, Yick TY, Ng GL, Wong WC (1992) Immunocytochemical localisation of substance P in vagal ganglion cells and pericellular arborisations in the monkey. J Anat 181:61–71
Ljungdahl A, Hökfelt T, Nilsson G (1978) Distribution of substance P-like immunoreactivity in the central nervous system of the rat. I. Cell bodies and nerve terminals. Neuroscience 3:861–943
Lundberg JM, Hökfelt T, Nilsson G, Terenius L, Rehfeld J, Elde R, Said S (1978) Peptide neurons in the vagus, splanchnic and sciatic nerves. Acta Physiol Scand 104:499–501
Maciewicz R, Phipps BS, Poote WE, Aronin N, Difiglia M (1983) The distribution of substance P-containing neurons in the cat Edinger Westphal nucleus: relationship to efferent projection system. Brain Res:270:217–230
Mantyh PW, Hunt SP (1984) Neuropeptides are present in projection neurons at all levels in visceral and taste pathways: from periphery to sensory cortex. Brain Res 299:297–311
Pernow B (1985) Substance P: present status and future prospects. In: Jordan CC, Oehme P (eds) Substance P metabolism and biological actions. Taylor and Francis, London Philadelphia, pp 187–196
Reiner A (1987) A VIP-like peptide co-occurs with substance P and enkephalin in cholinergic preganglionic terminals of the avian ciliary ganglion. Neurosci Lett 78:22–28
Reiner A, Erichsen JT, Cabot JB, Evinger C, Fitzgerald MEC, Karten HJ (1991) Neurotransmitter organization of the nucleus of Edinger-Westphal and its projection to the avian ciliary ganglion. Visual Neurosci 6:451–472
Ryall RW (1982) Modulation of cholinergic transmission by substance P. In: Substance P in the nervous system. Ciba Foundation Symposium 91. Pitman, London, pp 267–280
Sakanaka M, Shiosaka S, Takatsuki K, Inagaki S, Takagi H, Senba E, Kawai Y, Hara Y, Iida H, Minagawa H, Matsuzaki T, Tohyama M (1981) Evidence for the existence of a substance P-containing pathway from the nucleus laterodorsalis tegmenti (Castaldi) to the lateral septal area of the rat. Brain Res 240:351–355
Stone RA, Kuwayama Y (1985) Substance P-like immunoreactive nerves in the human eye. Arch Ophthalmol 103:1207–1211
Stone RA, Laties AM, Brecha NC (1982) Substance P-like immunoreactive nerves in the anterior segment of the rabbit, cat, and monkey eye. Neuroscience 7:2459–2468
Strobbia E, Corvetti G, Daneo S (1988) Distribution of substance P immunoreactive cell bodies and fibres in cranial sensory and autonomic ganglia of the chick. Basic Appl Histochem 32:161–167
Tay SSW, Wong WC (1992) Immunocytochemical localisation of substance P-like nerves in the cardiac ganglia of the monkey (Macaca fascicularis) J Anat 180:239–245
Wharton J, Polak TM, McGregor GP, Bishop AE, Bloom SR (1981) The distribution of substance P-like immunoreactive nerves in the guinea-pig heart. Neuroscience 6:2193–2204
White JD, Krause JE, Karten HJ, Mokelvy JF (1985) Presence and ontogeny of enkephalin and substance P in the chick ciliary ganglion. J Neurochem 45:1319–1322
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Zhang, Y.L., Tan, C.K. & Wong, W.C. Localisation of substance P-like immunoreactivity in the ciliary ganglia of monkey (Macaca fascicularis) and cat: a light- and electron-microscopic study. Cell Tissue Res 276, 163–171 (1994). https://doi.org/10.1007/BF00354796
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DOI: https://doi.org/10.1007/BF00354796