Abstract
Root extracts of leek (Allium porrum L.) and soybean (Glycine max L. Merr.) showed trehalase activity which was inhibited by phloridzin and was several times higher than the activity of general α-glucosidase. The activity had an acidic optimum. Trehalase activity in extracts of sporocarps and extraradical mycelium of the arbuscular mycorrhizal fungus Glomus mosseae Nicol. & Gerd. (Trappe & Gerd.) was higher than in root extracts and had an optimum at pH 7. Following inoculation with G. mosseae, trehalase activity increased in mycorrhizal roots above the levels observed in nonmycorrhizal roots. Irrespective of fungal colonization, root trehalase activity increased in the presence of Mg2+, decreased in the presence of Mn2+ and Zn2+, and was unaffected by Na2EDTA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bécard GL, Doner W, Rolin DB, Douds DD, Pfeffer DE (1991) Identification and quantification of trehalose in vesicular-arbuscular fungi by in vivo 13C NMR and HPLC analyses. New Phytol 118:547–552
Bethelenfalvay GJ, Brown MS, Pacovsky RS (1982) Relationships between host and endophyte development in mycorrhizal soybean. New Phytol 90:537–543
Cooper KM, Lösel D (1978) Lipid physiology of vesicular-arbuscular mycorrhiza. I. Composition of lipids in roots of onion, clover, and ryegrass infected with Glomus mosseae. New Phytol 80:143–151
Elbein AD (1974) The metabolism of trehalose. Adv Carbohydr Chem Biochem 30:227–256
Gerdemann JW, Nicolson TH (1963) Spores of mycorrhizal Enogone species extracted by wet sieving and decanting. Trans Br Mycol Soc 46:235–244
Glasziou KT, Gayler KR (1969) Sugar transport: occurrence of trehalase activity in sugarcane. Planta 85:299–302
Gussin AES (1972) Does trehalose occur in the Angiospermae? Phytochemistry 11:1827–1828
Gussin AES, McCormack JH (1970) Trehalase and the enzymes of trehalose biosynthesis in Lilium longiflorum pollen. Phytochemistry 9:1915–1920
Ho I, Trappe JM (1973) Translocation of 14C from Festuca plants to their endomycorrhizal fungi. Nature (London) 244:30–34
Kinden DA, Brown MF (1975) Electron microscopy of vesicul-ararbuscular mycorrhizae of yellow poplar. III. Intracellular hyphae and vesicles. Can J Microbiol 21:1768–1780
Lewis DH, Harley JH (1965) Carbohydrate physiology of mycorrhizal roots of beech. I. Identity of endogenous sugars and utilization of exogenous sugars. New Phytol 64:224–237
Lopez MF, Torrey JG (1985) Purification and properties of trehalase in Frankia ArI3. Arch Microbiol 143:209–215
Mellor RB (1988) Distribution of trehalase in soybean root nodule cells: implications for trehalose metabolism. J Plant Physiol 133:173–177
Mellor RB (1992) Is trehalose a symbiotic determinant in symbioses between higher plants and microorganisms? Symbiosis 12:113–129
Müller J, Staehelin C, Mellor RB, Boller T, Wiemken A (1992) Partial purification and characterization of trehalase from soybean nodules. J Plant Physiol 140:8–13
Schubert A, Wyss P, Wiemken A (1992) Occurrence of trehalose in vesicular-arbuscular mycorrhizal fungi and in mycorrhizal roots. J Plant Physiol 140:41–45
Streeter JG (1982) Enzymes of sucrose, maltose, and α, α-trehalose catabolism in soybean plant nodules. Planta 155:112–115
Streeter JG (1985) Accumulation of α,α trehalose by Rhizobium bacteria and bacteroids. J Bacteriol 164:78–84
Thevelein JM (1984) Regulation of trehalose mobilization in fungi. Microbiol Rev 48:42–59
Wyss P, Boller T, Wiemken A (1991) Phytoalexin response is elicited by a pathogen (Rhizoctonia solani) but not by a mycorrhizal fungus (Glomus mosseae) in soybean roots. Experientia 47:395–399
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schubert, A., Wyss, P. Trehalase activity in mycorrhizal and nonmycorrhizal roots of leek and soybean. Mycorrhiza 5, 401–404 (1995). https://doi.org/10.1007/BF00213439
Issue Date:
DOI: https://doi.org/10.1007/BF00213439