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HLA class II is associated with the frequency of glutamic acid decarboxylase M r 65 000 autoantibodies in Japanese patients with insulin-dependent diabetes mellitus

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Abstract

Autoantibodies to glutamic acid decarboxylase (GAD65Ab) are common in both caucasian and Japanese patients with insulin-dependent diabetes mellitus (type 1), while the type 1-associated HLA haplotypes differ. In the present study, we analyzed GAD65Ab in relation to HLA-DQ and-DR alleles in Japanese type 1 patients. GAD65Ab were found in 58% short-duration (less than 5 years) type 1,23% long-duration type 1,56% slowly progressive type 1,3% type 2 patients, and 1.7% healthy individuals. In 75 HLA-typed type 1 patients, the GAD65Ab frequency was higher in short-duration patients with DRB1*08 allele (100%,Pc<0.05). GAD65Ab frequencies in DQB1*0302, DQB1*0303, and DRB1*09-positive, long-duration type 1 patients were lower than those in short-duration type 1 patients (14%, 19%, and 20%,Pc<0.02 compared with short-duration type 1, 90%, 75%, and 71%, respectively), while the frequency varied less in DQB1*04 individuals (44% and 30% in short- and long-duration type 1 patients, respecitively). These findings were also observed among patients with DRB1*04, i.e., the haplotype DRB1*0405-DQB1*0401 showed less variation in frequency of GAD65Ab (44% and 35% in short- and long-duration type 1 patients, respectively), while DRB1*04xx-DQB1*0302 showed lower frequency in long-duration type 1 than short-duration (13% and 100%, respectively). Thus, HLA class II is associated with frequency GAD65Ab, and this association might be affected by disease duration in Japanese type 1 patients.

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Authors and Affiliations

  1. Department of Molecular Medicine, Karolinska Institute, Stockholm, Sweden

    A. Kasuga, A. Falorni & Å. Lernmark

  2. Department of Internal Medicine, Keio University School of Medicine, 35-Shinanomachi, Shinjuku-ku, 160, Tokyo, Japan

    A. Kasuga, Y. Ozawa, I. Takei & T. Saruta

  3. Department of Internal Medicine, Social Insurance Saitama Chuo Hospital, Saitama, Japan

    T. Maruyama

  4. Department of Medicine, University of Washington, Seattle, USA

    C. E. Grubin

  5. Clinical Science Labs., Diagnostics Technology Laboratory, Chugai Pharmaceutical, Tokyo, Japan

    K. Matsubara

  6. Department of Clinical Immunology, Karolinska Institute, Stockholm, Sweden

    A. Scheynius

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  1. A. Kasuga
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  2. A. Falorni
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  3. T. Maruyama
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  4. Y. Ozawa
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  5. C. E. Grubin
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  7. I. Takei
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  8. T. Saruta
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Kasuga, A., Falorni, A., Maruyama, T. et al. HLA class II is associated with the frequency of glutamic acid decarboxylase M r 65 000 autoantibodies in Japanese patients with insulin-dependent diabetes mellitus. Acta Diabetol 33, 108–113 (1996). https://doi.org/10.1007/BF00569419

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  • DOI: https://doi.org/10.1007/BF00569419

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