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In vitro activity of daptomycin-metronidazole combinations against mixed bacterial cultures: Reduced activity of metronidazole againstBacteroides species in the presence ofEnterococcus faecalis

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Abstract

The in vitro activity of daptomycin-metronidazole combinations against mixed cultures of gram-positive facultative cocci and strains of theBacteroides fragilis group was investigated. Metronidazole did not influence the high activity of daptomycin against strains ofStaphylococcus aureus, Staphylococcus epidermidis andEnterococcus faecalis in the absence or presence of the co-culturedBacteroides strains. In contrast, theEnterococcus faecalis isolates protected the co-culturedBacteroides strains against the killing effect of metronidazole, even at a concentration four- or eightfold the MIC of metronidazole. Killing curve experiments confirmed this protective effect of different isolates ofEnterococcus faecalis.

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References

  1. Jorgensen JH, Maher LA, Redding JS: In vitro activity of LY 146032 (daptomycin) against selected aerobic bacteria. European Journal of Clinical Microbiology 1987, 6: 91–95.

    PubMed  Google Scholar 

  2. Washington JA, Suter VL: Dilution susceptibility test: agar and macro-broth dilution procedures. In: Lennette EH, Balows A, Hausler WJ, Truant JP (ed): Manual of clinical microbiology. American Society for Clinical Microbiology, Washington, DC, 1980, p. 453–548.

    Google Scholar 

  3. Heizmann WR, Schmidt R, Heilmann F, Werner H: Association experiments with aerobic and anaerobic pathogens: a model of in vitro susceptibility testing in mixed infections. Activity of enoxacin, clindamycin and metronidazole. Infection 1989, 17: 160–164.

    PubMed  Google Scholar 

  4. Werner H.: Kombination von Acylureidopenicilinen mit Metronidazol bei Anaerobier und anaerobaeroben Mischkulturen. In: Wiedemann B, Hengstmann J, Adam D (ed): Securopen, Baypen: Simultane Anwendung mit anderen Wirkstoffen. Schattauer Verlag, Stuttgart, 1984, p. 25–40.

    Google Scholar 

  5. Soriano F, Ponte MC, Santamaria M: Activity of cefotaxime and metronidazole againstBacteroides andEscherichia coli alone and in mixed culture. Journal of Antimicrobial Chemotherapy 1983, 12: 89–92.

    Google Scholar 

  6. Soriano F, Ponte MC: Comparative activities of aztreonam and cefotaxime againstEscherichia coli andBacteroides species in pure and mixed cultures. Antimicrobial Agents and Chemotherapy 1984, 26: 39–41.

    PubMed  Google Scholar 

  7. Gisby J, Beal AS: Comparative efficacies of amoxicillin-clavulanic acid and ampicillin-sulbactam against experimentalBacteroides fragilis/Escherichia coli mixed infections., Antimicrobial Agents and Chemotherapy 1988, 32: 1830–1833.

    PubMed  Google Scholar 

  8. Houvinen P, Kotilainen P: In vitro activity of a new cyclic lipopeptide antibiotic LY 146032, against grampositive clinical bacteria. Antimicrobial Agents and Chemotherapy 1987, 31: 455–457.

    PubMed  Google Scholar 

  9. Chrystal EJT, Koch RL, McLafferty MA, Goldman P: Relationship between metronidazole metabolism and bactericidal activity. Antimicrobial Agents and Chemotherapy 1980, 18: 566–573.

    PubMed  Google Scholar 

  10. McLafferty MA, Koch RL, Goldman P: Interaction of metronidazole with resistant and susceptibleBacteroides fragilis. Antimicrobial Agents and Chemotherapy 1982, 21: 131–134.

    PubMed  Google Scholar 

  11. Soriano F, Ponte MC, Gaspar MC: Reciprocal antimicrobial synergism betweenEscherichia coli andBacteroides fragilis in the presence of metronidazole. Journal of Clinical Pathology 1982, 35: 1150–1152.

    PubMed  Google Scholar 

  12. Edwards DI: Mechanism of antimicrobial action of metronidazole. Journal of Antimicrobial Chemotherapy 1979, 5: 499–502.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Microbiology, Albert Szent-Györgyi Medical School, PO Box 482, 6701, Szeged, Hungary

    E. Nagy

  2. Department of Medical Microbiology, University of Tübingen, Silcherstr. 7, FRG

    H. Werner & W. Heizmann

Authors
  1. E. Nagy
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  2. H. Werner
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  3. W. Heizmann
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Nagy, E., Werner, H. & Heizmann, W. In vitro activity of daptomycin-metronidazole combinations against mixed bacterial cultures: Reduced activity of metronidazole againstBacteroides species in the presence ofEnterococcus faecalis . Eur. J. Clin. Microbiol. Infect. Dis. 9, 287–291 (1990). https://doi.org/10.1007/BF01968064

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