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Disk diffusion susceptibility testing ofHaemophilus influenzae using Haemophilus test medium

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Abstract

A three-center collaborative study was performed with the aim of determining the reproducibility of disk diffusion and broth microdilution susceptibility tests ofHaemophilus influenzae with 19 different antimicrobial agents using a newly described medium, Haemophilus Test Medium. The results of both methods were found to be highly reproducible. Using Haemophilus Test Medium, inhibitory zone diameters and MICs were then determined for 150 test strains ofHaemophilus influenzae, selected with an emphasis on known mechanisms of resistance. Regression analysis of zone diameter-MIC comparisons was used to define zone diameter interpretive criteria and MIC correlates for Haemophilus Test Medium disk diffusion tests performed withHaemophilus spp. for the following antimicrobial agents: ampicillin, amoxicillin/clavulanate, ampicillin/sulbactam, cefaclor, cefuroxime, cefamandole, ceftriaxone, cefotaxime, ceftizoxime, ceftazidime, cefixime, trimethoprim/sulfamethoxazole, rifampin, chloramphenicol, tetracycline, imipenem, aztreonam and ciprofloxacin.

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Authors and Affiliations

  1. Department of Clinical Microbiology and Division of Infectious Disease, University of Massachusetts Medical Center, 55 Lake Avenue, 01655, North, Worcester, Massachusetts, USA

    G. V. Doern & H. Snapper

  2. Department of Pathology, University of Texas Health Science Center, 78284, San Antonio, Texas, USA

    J. H. Jorgensen

  3. Antimicrobics Investigation Section, Centers for Disease Control, 3033, Atlanta, Georgia, USA

    C. Thornsberry

Authors
  1. G. V. Doern
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  2. J. H. Jorgensen
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  3. C. Thornsberry
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  4. H. Snapper
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Doern, G.V., Jorgensen, J.H., Thornsberry, C. et al. Disk diffusion susceptibility testing ofHaemophilus influenzae using Haemophilus test medium. Eur. J. Clin. Microbiol. Infect. Dis. 9, 329–336 (1990). https://doi.org/10.1007/BF01973739

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  • DOI: https://doi.org/10.1007/BF01973739

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