Summary
The adherence ofEntamoeba histolytica trophozoites to target cells was studied using a monoclonal antibody to a major surface antigen of 66 kD. Preincubation of trophozoites with monoclonal antibody decreased their ability to adhere to and engulf erythrocytes and to destroy Chinese hamster ovary cells. The monoclonal antibody was specific for the 66 kD antigen, which is possibly a major participant in the adhesion that precedes phagocytosis and cytopathic effects.
Zusammenfassung
Die Adhäsionsfähigkeit vonEntamoeba histolytica an Zielzellen unter dem Einfluß eines gegen das 66 kD Haupt-Oberflächenantigen gerichteten monoklonalen Antikörpers wurde untersucht. Nach Präinkubation mit dem monoklonalen Antikörper verminderte sich die Fähigkeit der Trophozoiten, sich an Erythrozyten anzuheften und Chinese hamster ovary (CHO)-Zellen zu zerstören. Der monoklonale Antikörper erwies sich als spezifisch für das 66 kD Antigen, das wahrscheinlich entscheidende Bedeutung für die Adhäsion hat, die anderen Vorgängen wie der Phagozytose und zytopathischen Effekten vorausgeht.
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References
Chadee, K., Meerovitch, E. The pathogenesis of experimentally induced amebic liver abscess in the gerbil (Meriones unguiculatus). Am. J. Pathol. 117 (1984) 71–80.
Ravdin, J. I., John, J. E., Johnston, L. I., Innex, D. J., Guerrant, R. L. Adherence ofEntamoeba histolytica tropozoites to rat and human colonic mucosa. Infect. Immun. 48 (1985) 292–297.
Ravdin, J. I., Petri, W. A., Murphy, C., Smith, R. D. Production of those monoclonal antibodies which inhibitin vitro adherence inEntamoeba histolytica trophozoites. Infect. Immun. 53 (1986) 1–5.
Arroyo, R., Orozco, E. Localization and identification of anEntamoeba histolytica adhesin. Mol. Biochem. Parasitol. 23 (1987) 151–158.
Diamond, L. S., Marlow, D. R., Cunnick, C. C. A new medium for the axenic cultivation ofEntamoeba histolytica and other entamoeba. Trans. R. Soc. Trop. Med. Hyg. 72 (1978) 431–432.
Aley, S. B., Scott, W. A., Cohn, Z. A. Plasma membrane ofEntamoeba histolytica. J. Exp. Med. 152 (1980) 391–404.
Engvall, E., Perlman, P. Enzyme linked immunosorbent assay (ELISA) quantitative assay for immunoglobulin G. Immunochemistry 8 (1971) 871–876.
Orozco, E., Guarneros, G., Martinez-Polomo, A., Sanchez, T. Entamoeba histolytica: Phagocytosis as virulence factor. J. Exp. Med. 158 (1983) 1511–1521.
Bracha, R., Mirelman, D. Virulence ofEntamoeba histolytica trophozoites. Effect of bacteria; microaerobic conditions and metronidazole. J. Exp. Med. 160 (1984) 353–368.
Parham, P., Androlewicz, M. J., Brodsky, F. M., Holmes, J. N., Ways, J. P. Monoclonal antibodies: purification, fragmentation and application to structural and functional studies of class I MHC antigens. J. Immunol. Methods 53 (1983) 133–173.
Towbin, H. T., Stakelin, N., Gordon, J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76 (1979) 4350–4354.
Aust Kettis, A., Thorstensson, R., Ulter, G. Antigenicity ofE. histolytica strain NIH:200: a survey of clinically relevant antigenic components. Am. J. Trop. Med. Hyg. 32 (1983) 512–516.
Torian, B. E., Lukehart, S. A., Stamm, W. A. Use of monoclonal antibody to identify, characterize and purify a 96,000 dalton surface antigen of pathogenicEntamoeba histolytica. J. Infect. Dis. 156 (1987) 334–339.
Orozco, E., Arroyo, R., Rodriguez, M. A., Garcia-Rivera, G. Identification of anEntamoeba histolytica adhesion using adhesion deficit mutants and monoclonal antibodies. In:Agabian, N., Gordwan, H., Nogueira, N. (eds.): Molecular strategies of parasitic invasion. Alan R. Liss, New York 1987.
Chadee, K., Petri, W. A., Innes, D. J., Ravdin, J. I. Rat and human colonic mucins bind to and inhibit adherence lectin ofEntamoeba histolytica. J. Clin. Invest. 80 (1987) 1245–1254.
Meza, I., Cazanes, F., Rosales-Encina, J. L., Talawas, P., Rojkind, M. Use of antibodies to characterize a 220 kilodalton protein fromEntamoeba histolytica. J. Infect. Dis. 156 (1987) 798–802.
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Vohra, H., Kaur, S., Wangoo, A. et al. Inhibition of target cell adhesion by monoclonal antibody to the 66 kD surface antigen ofEntamoeba histolytica . Infection 20, 279–282 (1992). https://doi.org/10.1007/BF01710795
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DOI: https://doi.org/10.1007/BF01710795