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In vitro assessment of the haemolytic potential of candidate drugs

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Abstract

Several chemicals cause haemolysis when administered to mammals by the intravenous route. From a regulatory point of view there are no recommended methods for assessing the haemolytic potential of candidate drugs. In our laboratory, we routinely investigate the reaction between human (or animal) whole blood and candidate drugs (or their vehicles or metabolites) and measure haemolysis by assaying haemoglobin in the supernatants. Here, we describe this method. This test is inexpensive and requires only few millilitres of whole blood.

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References

  • King EJ, Gilchrist M (1947) Determination of hemoglobin by a cyanhematin method. Lancet ii:201–202

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Authors and Affiliations

  1. Rhône-Poulenc Rorer SA, Drug Safety Department, BP14, 94403, Vitry Cedex, France

    D. Salauze & D. Decouvelaere

Authors
  1. D. Salauze
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  2. D. Decouvelaere
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Salauze, D., Decouvelaere, D. In vitro assessment of the haemolytic potential of candidate drugs. Comp Haematol Int 4, 34–36 (1994). https://doi.org/10.1007/BF00368264

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  • DOI: https://doi.org/10.1007/BF00368264

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