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Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy

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Abstract

The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP), porphyrinc (Pc) and the tetrakiscarborane carboxylate ester of 2,4-(α,β-dihydroxyethyl) deuteroporphyrin IX (BOPP). From the heterogeneous emission observed in vitro, assignments and spatial location of various porphyrin species are proposed.

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Authors and Affiliations

  1. School of Chemistry, University of Melbourne, 3052, Parkville, Victoria, Australia

    P. G. Spizzirri & K. P. Ghiggino

  2. Melbourne Neuroscience Centre, Royal Melbourne Hospital, 3052, Parkville, Victoria, Australia

    J. S. Hill

  3. Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, 94143-0446, San Francisco, CA, USA

    S. B. Kahl

Authors
  1. P. G. Spizzirri
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  2. J. S. Hill
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  3. S. B. Kahl
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  4. K. P. Ghiggino
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Spizzirri, P.G., Hill, J.S., Kahl, S.B. et al. Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy. Laser Med Sci 11, 237–246 (1996). https://doi.org/10.1007/BF02134914

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  • DOI: https://doi.org/10.1007/BF02134914

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