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Ultrastructure of differentiating preameloblasts from tooth germs of the permanent dentition ofMacaca mulatta andMacaca arctoides

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Summary

Cytodifferentiation of inner enamel epithelium and the adjacent connective tissue from the tip of the cervical loop to the initiation of enamel elaboration in twoMacaca species was examined. Ten- to twelve-month-old specimens were fixed by perfusion and the permanent tooth buds were prepared for transmission electron microscopy. At the cervical loop proper, inner enamel epithelium cells have lobed nuclei, a paucity of cytoplasm, and wide extracellular spaces; the basal lamina facing the dental papilla is straight.

With increasing distance from the tip of the cervical loop, the following changes occur gradually: (a) preameloblasts elongate from 15 to 45 µm, and their organelles, particularly mitochondria and profiles of rough endoplasmic reticulum, become more numerous; (b) extracellular spaces decrease between preameloblasts starting at the basal (infranuclear) end; (c) the basement membrane becomes convoluted and associated with aperiodic fibers; (d) preodontoblast projections penetrate the aperiodic fibers; (e) collagen fibers subjacent to the basement membrane increase in density, with particularly thick fibers paralleling the aperiodic fibers. These modifications occur within three-fourths of the distance from the tip of the cervical loop to the mineralization front.

The condensation of preodontoblasts is followed immediately by predentin synthesis. Concomitantly, the basement membrane breaks down and the aperiodic fibers are engulfed by preameloblasts. Preameloblast projections penetrate junctional predentin, contact mineralized dentin, and enamel synthesis ensues. At this stage the ameloblast is 45 µm long, the nucleus is central or basal, the Golgi apparatus has migrated apically, but the Tomes' process has not yet formed.

The results indicate that odontogenesis inMacaca monkeys more closely resembles human odontogenesis than does that in the murine rodents.

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References

  1. Boyde, A.: Amelogenesis and the structure of enamel. In B. Cohen, I. R. H. Kramer (eds.): Scientific Foundations of Dentistry, pp. 335–352. Year Book Medical Publishers, Chicago, 1976

    Google Scholar 

  2. Kallenbach, E.: Fine structure of secretory ameloblasts in the kitten, Am. J. Anat.148:479–511, 1977

    Article  CAS  PubMed  Google Scholar 

  3. Skobe, Z.: Enamel rod formation in the monkey observed by scanning electron microscopy, Anat. Rec.187:329–333, 1977

    Article  CAS  PubMed  Google Scholar 

  4. Kallenbach, E.: Fine structure of differentiating ameloblasts in the kitten, Am. J. Anat.145:283–317, 1976

    Article  CAS  PubMed  Google Scholar 

  5. Ronnholm, E.: An electron microscopic study of the amelogenesis in human teeth. I. The fine structure of the ameloblasts, J. Ultrastruct. Res.6:229–248, 1962

    Article  CAS  PubMed  Google Scholar 

  6. Ronnholm, E.: The amelogenesis of human teeth as revealed by electron microscopy. II. The development of the enamel crystallites, J. Ultrastruct. Res.6:249–303, 1962

    Article  CAS  PubMed  Google Scholar 

  7. Matthiessen, M. E., von Bülow, F. A.: The ultrastructure of human secretory ameloblasts, Z. Zellforsch.101:232–240, 1969

    Article  CAS  PubMed  Google Scholar 

  8. Sisca, R. F., Provenza, D. V.: Initial dentin formation in human deciduous teeth, Calcif. Tissue Res.9:1–16, 1972

    Article  CAS  PubMed  Google Scholar 

  9. Provenza, D. V., Sisca, R. F.: The dental primordium, an electron microscopic study of the cervical loop, J. Periodontol.44:551–558, 1973

    CAS  PubMed  Google Scholar 

  10. Yama, K.: Ultrastructure of human ameloblasts, J. Tokyo Dent. Coll. Soc.6:117–160, 1971

    Google Scholar 

  11. Boyde, A.: The development of enamel structure, Proc. R. Soc. Med.60:923–928, 1967

    CAS  PubMed  Google Scholar 

  12. Frank, R. M., Nalbandian, J.: Ultrastructure of amelogenesis. In A. E. W. Miles (ed.): Structural and Chemical Organization of Teeth, Vol. I, pp. 399–466. Academic Press, New York, 1967

    Google Scholar 

  13. Silva, D. G., Kailis, D. G.: Ultrastructural studies on the developing tooth in normal cats and cats exposed to low levels of fluoride. I. The cervical loop, early dentinogenesis and initial amelogenesis, Aust. Dent. J.17:123–131, 1972

    Article  CAS  PubMed  Google Scholar 

  14. Silva, D. G., Kailis, D. G.: Ultrastructural studies on the cervical loop and the development of the amelo-dentinal junction in the cat, Arch. Oral Biol.17:279–289, 1972

    Article  CAS  PubMed  Google Scholar 

  15. Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy, J. Cell Biol.27:137A, 1965, (abst.)

  16. Warshawsky, H., Moore, G.: A technique for the fixation and decalcification of rat incisors for electron microscopy, J. Histochem. Cytochem.15:542, 1967

    CAS  PubMed  Google Scholar 

  17. Slavkin, H. C., Bringas, P., LeBaron, R., Cameron, J., Bavetta, L. A.: The fine structure of the extracellular matrix during epitheliomesenchymal interactions in the rabbit embryonic incisor, Anat. Rec.165:237–255, 1969

    Article  CAS  PubMed  Google Scholar 

  18. Katchburian, E., Holt, S. J.: Studies on the development of ameloblasts. I. Fine structure, J. Cell Sci.11:415–447, 1972

    CAS  PubMed  Google Scholar 

  19. Zajicek, G., Michaeli, Y., Weinreb, M.: Kinetics of the inner enamel epithelium in the adult rat incisor during accelerated eruption, Cell Tissue Kinet.5:35–39, 1972

    CAS  PubMed  Google Scholar 

  20. Kallenbach, E.: Electron microscopy of the differentiating rat incisor ameloblast, J. Ultrastruct. Res.35:508–531, 1971

    Article  CAS  PubMed  Google Scholar 

  21. Orams, J. H.: The ultrastructure of tissues at the epithelial-mesenchymal interface in developing rat incisors, Arch. Oral Biol.23:39–44, 1978

    Article  CAS  PubMed  Google Scholar 

  22. Hay, E. D., Revel, J. P.: Fine structure of the developing avian cornea. In A. Wolsky, P. S. Chen (eds.): Monographs in Developmental Biology, Vol. I, pp. 119–129. S. Karger, Basel, 1969

    Google Scholar 

  23. Slavkin, H. C., Matosian, P., Wilson, P., Bringas, P., Mino, W., Croissant, R. D., Guenther, H.: Epithelial-specific extracellular matrix influences on mesenchyme collagen biosynthesisin vitro. In H. C. Slavkin, R. C. Greulich (eds.): Extracellular Matrix Influences on Gene Expression, pp. 237–251. Academic Press, New York, 1975

    Google Scholar 

  24. Trelstad, R. L., Slavkin, H. C.: Collagen synthesis by the epithelial enamel organ of the embryonic rabbit tooth, Biochem. Biophys. Res. Commun.59:443–449, 1974

    Article  CAS  PubMed  Google Scholar 

  25. Reith, E. J.: The early stage of amelogenesis as observed in molar teeth of young rats, J. Ultrastruct. Res.17:503–526, 1967

    Article  CAS  PubMed  Google Scholar 

  26. Takuma, S.: Ultrastructure of dentinogenesis. In A. E. W. Miles (ed.): Structural and Chemical Organization of Teeth, Vol. I., pp. 325–370. Academic Press, New York, 1967

    Google Scholar 

  27. Weinstock, M., Leblond, C. P.: Synthesis, migration, and release of precursor collagen by odontoblasts as visualized by radioautography after [3H] proline administration, J. Cell Biol.60:92–127, 1974

    Article  CAS  PubMed  Google Scholar 

  28. Reith, E. J.: Collagen formation in developing molar teeth of rats, J. Ultrastruct. Res.21:383–414, 1968

    Article  Google Scholar 

  29. Garant, P. R., Szabo, G., Nalbandian, J.: The fine structure of the mouse odontoblast, Arch. Oral Biol.13:857–876, 1968

    Article  CAS  PubMed  Google Scholar 

  30. Katchburian, E., Burgess, A. M. C.: Fine structure of contacts between ameloblasts and odontoblasts in the rat tooth germ, Arch. Oral Biol.22:551–553, 1977

    Article  CAS  PubMed  Google Scholar 

  31. Osborn, J. W.: The relationship between prisms and enamel tubules in the teeth ofDidelphis marsupialis, and the probable origin of the tubules, Arch. Oral Biol.19:835–844, 1974

    Article  CAS  PubMed  Google Scholar 

  32. Koch, W. E.:In vitro differentiation of tooth rudiments of embryonic mice. I. Transfilter interaction of embryonic incisor tissues, J. Exp. Zool.165:155–169, 1967

    Article  CAS  PubMed  Google Scholar 

  33. Wolters, J. M. L., van Mullem, P. J.: Electron microscopy of epitheliomesenchyme intercellular communication in trans-filter cultures of rat tooth germs, Arch. Oral Biol.22:705–709, 1977

    Article  CAS  PubMed  Google Scholar 

  34. Garant, P. R.: Microanatomy of the oral mineralized tissues. In J. H. Shaw, E. A. Sweeney, C. C. Cappuccino, S. M. Meller (eds.): Textbook of Oral Biology, pp. 181–225. W. B. Saunders Co., Philadelphia, 1978

    Google Scholar 

  35. Bernard, G. W.: Ultrastructural observations of initial calcification in dentine and enamel, J. Ultrastruct. Res.41:1–17, 1972

    Article  CAS  PubMed  Google Scholar 

  36. Thylstrup, A., Skaaring, P., Fejerskov, O., Bierring, F.: Surface structure of tooth germs from newborn infants: a light and scanning electron microscopical study, J. Anat.123:537–547, 1977

    CAS  PubMed  Google Scholar 

  37. Watson, M. L.: The extracellular nature of enamel in the rat, J. Biophys. Biochem. Cytol.7:489–497, 1960

    Article  CAS  PubMed  Google Scholar 

  38. Katchburian, E.: Membrane-bound bodies as initiators of mineralization of dentine, J. Anat.116:285–302, 1973

    CAS  PubMed  Google Scholar 

  39. Eisenmann, D. R., Glick, P. L.: Ultrastructure of initial crystal formation in dentin, J. Ultrastruct. Res.41:18–28, 1972

    Article  CAS  PubMed  Google Scholar 

  40. Whittaker, D. K.: The enamel-dentine junction of human andMacaca irus teeth: a light and electron microscopic study, J. Anat.125:323–335, 1978

    CAS  PubMed  Google Scholar 

  41. Boyde, A., Reith, E. J.: The pattern of mineralization of rat molar dentine, Z. Zellforsch.94:479–486, 1969

    Article  CAS  PubMed  Google Scholar 

  42. Nylen, M. U., Scott, D. B.: Electron microscopic studies of odontogenesis, J. Ind. St. Dent. Assoc.39:406–421, 1960

    Google Scholar 

  43. Yamada, M., Bringas, P., Jr., Slavkin, H. C.: Comparison ofin vivo andin vitro amelogenesis, J. Dent. Res.58, Special Issue A, #1170, 1979 (abst.)

  44. Garant, P. R., Nalbandian, J.: Observations on the ultrastructure of ameloblasts with special reference to the Golgi complex and related components, J. Ultrastruct. Res.23:427–443, 1968

    Article  CAS  PubMed  Google Scholar 

  45. Warshawsky, H.: The fine structure of secretory ameloblasts in rat incisors, Anat. Rec.161:211–229, 1968

    Article  CAS  PubMed  Google Scholar 

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  1. Forsyth Dental Center, 140 The Fenway, 02115, Boston, Massachusetts, USA

    Z. Skobe, D. Stern & K. Prostak

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Skobe, Z., Stern, D. & Prostak, K. Ultrastructure of differentiating preameloblasts from tooth germs of the permanent dentition ofMacaca mulatta andMacaca arctoides . Calcif Tissue Int 33, 603–618 (1981). https://doi.org/10.1007/BF02409498

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