Skip to main content
SpringerLink
Log in

Short-term bromide uptake in skins of Rana pipiens

  • Articles
  • Published:
The Journal of Membrane Biology Aims and scope Submit manuscript

Abstract

Intracellular ion concentrations were determined in split skins of Rana pipiens using the technique of electron microprobe analysis. Based on the 1 min Br uptake from the apical bath, two types of mitochondria-rich (MR) cells could be distinguished: active cells which rapidly exchanged their anions with the apical bath and inactive cells which did not. Br uptake and frequency of active MR cells were closely correlated with the skin conductance, g t. Replacing Cl in the apical bath with an impermeant anion significantly lowered g t and the Br uptake and Na concentration of active cells. Even larger reductions were observed after apical amiloride (0.1 mm). The inhibition of the Br uptake was reversible by voltage clamping (100 mV, inside positive). Cl removal and amiloride also led to some shrinkage of active cells. The results suggest that the active cell is responsible for a large part of g t. Inactive MR cells had much lower Br and Na concentrations which were not significantly affected by Cl removal, amiloride, or voltage clamping. Principal cells, which represent the main cell type of the epithelium, showed only a minimal Br uptake from the apical side which was not correlated with g t. Moreover, Cl removal had no effect on the Na, Br, and Cl concentrations of principal cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bauer, R., Rick, R. 1978. Computer analysis of x-ray spectra (EDS) from thin biological specimens. X-Ray Spectrom. 7:63–69

    Google Scholar 

  • Biber, T.U.L., Drewnowska, K., Baumgarten, C.M., Fisher, R.S. 1985. Intracellular Cl activity changes of frog skin. Am. J. Physiol. 249:F432–438

    Google Scholar 

  • Blatter, L.A. 1991. Estimation of intracellular free magnesium using ion-selective microelectrodes: Evidence for an Na/Mg exchange mechanism in skeletal muscle. Magnes. Trace Elem. 10:67–69

    Google Scholar 

  • Dörge, A., Rick, R., Beck, F.X., Nagel, W. 1988. Uptake of Br in mitochondria-rich and principal cells of toad skin epithelium. Pfluegers Arch. 412:305–313

    Google Scholar 

  • Dörge, A., Rick, R., Beck, F.X., Thurau, K. 1985. Cl transport across the basolateral membrane in frog skin epithelium. Pfluegers Arch. 405:S8-S11

    Google Scholar 

  • Drewnowska, K., Biber, T.U.L. 1985. Active transport and exchange diffusion of Cl across the isolated skin of Rana pipiens. Am. J. Physiol. 249:F424–431

    Google Scholar 

  • Ehrenfeld, J., Garcia-Romeu, F. 1977. Active hydrogen excretion and sodium absorption through isolated frog skin. Am. J. Physiol. 233:F46-F54

    Google Scholar 

  • Foskett, K.J., Ussing, H.H. 1986. Localization of chloride conductance to mitochondria-rich cells in frog skin epithelium. J. Membrane Biol. 91:251–258

    Google Scholar 

  • Harck, A.F., Larsen, E.H. 1986. Concentration dependence of halide fluxes and selectivity of the anion pathway in toad skin. Acta Physiol. Scand. 128:289–304

    Google Scholar 

  • Harvey, B.J. 1992. Energization of sodium absorption by the H+-AT-Pase pump in mitochondria-rich cells of frog skin. J. Exp. Biol. 172:289–309

    Google Scholar 

  • Katz, U., Scheffey, C. 1986. The voltage-dependent chloride conductance of toad skin is localized to mitochondria-rich cells. Biochim. Biophys. Acta 831:480–482

    Google Scholar 

  • Kristensen, P. 1983. Exchange diffusion, electrodiffusion, and rectification in the chloride transport pathway in frog skin. J. Membrane Biol. 72:141–151

    Google Scholar 

  • Larsen, E.H. 1991. Chloride transport by high-resistance heterocellular epithelia. Physiol. Rev. 71:235–284

    Google Scholar 

  • Larsen, E.H., Rasmussen, B.E. 1985. A mathematical model of amphibian skin epithelium with two types of transporting cellular units. Pfluegers Arch. 405:S50–58

    Google Scholar 

  • Larsen, E.H., Ussing, H.H., Spring, K.R. 1987. Ion transport by mitochondria-rich cells in toad skin. J. Membrane Biol. 99:25–40

    Google Scholar 

  • Nagel, W. 1989. Chloride conductance of frog skin: localization to the tight junctions? Miner. Electrolyte Metab. 15:163–70

    Google Scholar 

  • Nagel, W., Dörge, A. 1990. Analysis of anion conductance in frog skin. Pfluegers Arch. 416:53–61

    Google Scholar 

  • Rick, R. 1992. Intracellular ion concentrations in the isolated frog skin epithelium: Evidence for different types of mitochondria-rich cells. J. Membrane Biol. 127:227–238

    Google Scholar 

  • Rick, R., Dörge, A., Arnim, E. von, Thurau, K. 1978. Electron microprobe analysis of frog skin epithelium: evidence for a syncytial sodium transport compartment. J. Membrane Biol. 39:313–331

    Google Scholar 

  • Rick, R., Dörge, A., Beck, F.X., Thurau, K. 1986. Electron probe X-ray microanalysis of transepithelial ion transport. Ann. NY Acad. Sci. 483:245–259

    Google Scholar 

  • Rick, R., Dörge, A., Sesselmann, E. 1988. Na transport stimulation by novobiocin: transepithelial parameters and evaluation of ENa. Pfluegers Arch. 411:243–251

    Google Scholar 

  • Rick, R., Dörge, A., Thurau, K. 1982. Quantitative analysis of electrolytes in frozen dried sections. J. Microsc. 125:239–247

    Google Scholar 

  • Rick, R., Roloff, C., Dörge, A., Beck, F.X., Thurau, K. 1984. Intracellular electrolyte concentrations in the frog skin epithelium: Effect of vasopressin and dependence on the Na concentration in the bathing media. J. Membrane Biol. 78:129–145

    Google Scholar 

  • Spring, K.R., Ussing, H.H. 1986. The volume of mitochondria-rich cells of frog skin epithelium. J. Membrane Biol. 92:21–26

    Google Scholar 

  • Steinmetz, P.R. 1986. Cellular organization of urinary acidification. Am. J. Physiol. 251:F173–187

    Google Scholar 

  • Ussing, H.H., Windhager, E.E. 1964. Nature of shunt path and active sodium transport path through frog skin epithelium. Acta Physiol. Scand. 61:484–504

    Google Scholar 

  • Voûte, C.L., Meier, W. 1978. The mitochondria-rich cells of frog skin as hormone sensitive “shunt path”. J. Membrane Biol. 40:151–165

    Google Scholar 

  • Watlington, C.O., Sessee, F., Jr. 1973. Chloride-flux across frog skin of low potential difference. Biochim.Biophys. Acta 330:102–107

    Google Scholar 

  • Willumsen, N.J., Larsen, E.H. 1985. Passive Cl− currents in toad skin: potential dependence and relation to mitochondria-rich cell density. In: Transport Processes, Inoand Osmoregulation. R. Gilles, M. Gilles-Baillien, editors, pp. 20–30. Springer-Verlag, Berlin

    Google Scholar 

  • Willumsen, N.J., Larsen, E.H. 1986. Membrane potentials and intracellular Cl− activity of toad skin epithelium in relation to activation and deactivation of the transepithelial Cl− conductance. J. Membrane Biol. 94:173–190

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, Nephrology Research and Training Center, University of Alabama at Birmingham, 35294, Birmingham, Alabama

    R. Rick

Authors
  1. R. Rick
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

I wish to thank Cathy Langford for her excellent technical assistance. Financial support was provided by National Institutes of Health grants DK35717 and 1S10-RR0-234501.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rick, R. Short-term bromide uptake in skins of Rana pipiens . J. Membarin Biol. 138, 171–179 (1994). https://doi.org/10.1007/BF00232645

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232645

Key words

Search

Navigation