Summary
A large number of compounds were tested for their effectiveness as inhibitors of the tricarboxylate and dicarboxylate transporting systems of rat liver mitochondria as monitored by citrate/[14C] citrate exchange and phosphate/[14C]l-malate exchange, respectively. The influence of inhibitor structure on inhibitory potency is discussed for each transporting system and deductions made concerning the nature of the binding sites of these carrier systems.
Similar content being viewed by others
References
Chappell, J. B., Haarhoff, K. N. 1966. The penetration of the mitochondrial membrane by anions and cations.In: Biochemistry of Mitochondria E. C. Slater, Z. Kaniuga and L. Wojtcak, editors. p. 75. Academic Press Inc., New York, and Polish Scientific Publishers, Warsaw.
Chappell, J. B., Robinson, B. H. 1968. Penetration of the mitochondrial membrane by tricarboxylic acid anions.Biochem. Soc. Symp. 27:123.
Ferguson, S. M. F., Williams, G. R. 1966. The effect of malate and other dicarboxylic acids on mitochondrial isocitrate metabolism.J. Biol. Chem. 241:3969.
Prelog, V., Schneider, R. 1949. Untersuchungen über Organextrakte. Über die Lage der zweiten Carbonyl-Gruppe im Diketon D (Oxo-cis-tetrahydro-jonen) aus dem Harn trächtiger Stuten.Helv. Chim. Acta 32:1632.
Quagliariello, E., Palmieri, F., Prezioso, G., Klingenberg, M. 1969. Kinetics of succinate uptake by rat-liver mitochondria.F.E.B.S. 4:251.
Robinson, B. H., Chappell, J. B. 1967. The inhibition of malate, tricarboxylate and oxoglutarate entry into mitochondria by 2-n-butylmalonate.Biochem. Biophys. Res. Commun. 28:249.
Robinson, B. H., Chappell, J. B. 1970. The kinetics of tricarboxylate anion oxidation by rat liver mitochondria in relation to the availability ofl-malate.Biochim. Biophys. Acta 205:300.
Robinson, B. H., Williams, G. R. 1969. The effect on mitochondrial oxidations of inhibitors of the dicarboxylate anion transporting system.F.E.B.S. 5:301.
Robinson, B. H., Williams G. R. 1970. The sensitivity of dicarboxylate anion exchange reactions to transport inhibitors in rat-liver mitochondria.Biochim. Biophys. Acta 216:63.
Robinson, B. H., Williams, G. R., Halperin, M. L., Leznoff, C. C. 1970. The effects of 2-ethylcitrate and tricarballylate on citrate transport in rat liver mitochondria and fatty acid synthesis in rat white adipose tissue.Europ. J. Biochem. 15:263.
Robinson, B. H., Williams, G. R., Halperin, M. L., Leznoff, C. C. 1971. The sensitivity of the exchange reactions of tricarboxylate, 2-oxoglutarate and dicarboxylate transporting systems of rat liver mitochondria to inhibition of 2-pentylmalonatep-iodobenzylmalonate and benzene 1,2,3-tricarboxylate.Europ. J. Biochem. 20:65.
Robinson, B. H., Williams, G. R., Halperin, M. L., Leznoff, C. C. 1971. Factors affecting the kinetics and equilibrium of exchange reactions of the citrate-transporting system of rat liver mitochondria.J. Biol. Chem. 246:5280.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Robinson, B.H., Williams, G.R., Halperin, M.L. et al. Inhibitors of the dicarboxylate and tricarboxylate transporting systems of rat liver mitochondria. J. Membrain Biol. 7, 391–401 (1972). https://doi.org/10.1007/BF01867928
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01867928