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A kinetic analysis of the electrogenic pump ofChara corallina: II. Dependence of the pump activity on external pH

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Summary

The current-voltage curve of theChara membrane was obtained by applying a slow ramp de- and hyperpolarization by use of voltage clamp. By inhibiting the electrogenic pump with 50μm DCCD (dicyclohexylcarbodiimide), theI–V curve approached a steady state within 100 min, which gave thei d -V curve of the passive diffusion channel. Thei p -V curve of the electrogenic pump channel was obtained by subtracting the latter from the former. With the increase of external pH, thei d -V curve showed only a slight change, while thei p -V curve of the pump channel showed almost a parallel shift, in the hyperpolarizing direction, along the voltage axis in the pH range between 6.5 and 7.5. The sigmoidali p -V curve in this pH range could be simulated satisfactorily with the five-state model reported previously (U. Kishimoto, N. Kami-ike, Y. Takeuchi & T. Ohkawa,J. Membrane Biol. 80:175–183, 1984) as well as with a lumped two-state model presented in this report. The analysis based on these models suggests that the electrogenic pump of theChara membrane is mainly a 2H+/1ATP pump. The forward rate constant in the voltage-dependent step increased with the increase of external pH, while the backward one decreased. On the other hand, the forward rate constant in the voltage-independent step remained almost unchanged with the increase of external pH, while the backward one increased markedly. The pump conductance at the resting membrane potential showed either a slight increase or a decrease with the increase of external pH, depending on the sample. Nevertheless, the pump current showed generally a slight increase with the increase of external pH.

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Authors and Affiliations

  1. Department of Biology, College of General Education, Osaka University, 560, Toyonaka, Japan

    Y. Takeuchi, U. Kishimoto, T. Ohkawa & N. Kami-ike

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  1. Y. Takeuchi
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  2. U. Kishimoto
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  3. T. Ohkawa
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  4. N. Kami-ike
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Takeuchi, Y., Kishimoto, U., Ohkawa, T. et al. A kinetic analysis of the electrogenic pump ofChara corallina: II. Dependence of the pump activity on external pH. J. Membrain Biol. 86, 17–26 (1985). https://doi.org/10.1007/BF01871606

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  • DOI: https://doi.org/10.1007/BF01871606

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