Abstract
The mixed microbial flora of 3 lakes in Ohio with differing histories of hydrocarbon pollution was examined in relation to the ability to use hydrocarbons. Weathered kerosene was spiked with naphthalene, pristane, 1,13-tetradecadiene, andn-hexadecane and added to water-sediment mixtures from the 3 lakes, and utilization of the 4 marker hydrocarbons was measured. Each of the marker hydrocarbons was metabolized; naphthalene was the most readily used and pristane was the most resistant. Values for dissolved oxygen suggest that oxygen did not limit hydrocarbon degradation in the water column at any site examined. Nutrient addition studies indicated that nitrogen and phosphorus limited hydrocarbon degradation at all sites examined. Maximum numbers of heterotrophic bacteria were detected when the water temperature was 10°C or higher. The data indicate that temperature limits hydrocarbon degradation in the winter, except at a site which had been impacted by an oil spill and which received chronic inputs of hydrocarbons and nutrients. In samples from that site, all 4 marker hydrocarbons were degraded at 0°C. Results of temperature and nutrient-addition experiments suggest that different seasonal populations of hydrocarbon users are selected at that site, but not at other lake sites.
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References
Atlas RM (1981) Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev 45:180–208
Atlas RM, Bartha R (1972) Degradation and mineralization of petroleum in seawater: limitation by nitrogen and phosphorus. Biotechnol Bioeng 14:309–318
Atlas RM, Bartha R (1972) Biodegradation of petroleum in seawater at low temperatures. Can J Microbiol 18:1851–1855
Atlas RM, Busdosh M (1976) Microbial degradation of petroleum in the Arctic. In: Sharpley JM, Kaplan AM (eds) Proceedings of the Third International Biodegradation Symposium, Applied Science Publishers Ltd., London, pp 79–85
Bartha R, Atlas RM (1977) The microbiology of aquatic oil spills. Adv Appl Microbiol 22: 225–266
Cerniglia CE, Perry JJ (1973) Crude oil degradation by microorganisms isolated from the marine environment. Z Allg Microbiol 13:299–306
Cooney JJ (1984) The fate of petroleum pollutants in freshwater ecosystems. In: Atlas RM (ed) Petroleum microbiology, pp 399–433
Cooney JJ, Summers RJ (1976) Hydrocarbon-using microorganisms in three fresh-water ecosystems. In: Sharpley JM, Kaplan AM (eds) Proceedings of the Third International Biodegradation Symposium, Applied Science Publishers Ltd., London, pp 141–155
Foght JM, Westlake DWS (1982) Effect of dispersant Corexit 9527 on the microbial degradation of Prudhoe Bay oil. Can J Microbiol 28:117–122
Gibbs CF (1975) Quantitative studies of marine biodegradation of oil. I. Nutrient limitation at 14°C. Proc R Soc Lond B. (Biol Sci) 188:61–82
Hambrick GA III, DeLaune RD, Patrick WH Jr (1980) Effect of estuarine sediment pH and oxidation-reduction potential on microbial hydrocarbon degradation. Appl Environ Microbiol 40:365–369
Hughes DE, McKenzie P (1975) The microbial degradation of oil in the sea. Proc R Soc Lond B (Biol Sci) 189:375–390
Jamison VW, Raymond RL, Hudson JO Jr (1975) Biodegradation of high-octane gasoline in groundwater. Dev Ind Microbiol 16:305–312
Ward DM, Brock TD (1976) Environmental factors influencing the rate of hydrocarbon oxidation in temperate lakes. Appl Environ Microbiol 31:764–772
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Cooney, J.J., Silver, S.A. & Beck, E.A. Factors influencing hydrocarbon degradation in three freshwater lakes. Microb Ecol 11, 127–137 (1985). https://doi.org/10.1007/BF02010485
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DOI: https://doi.org/10.1007/BF02010485