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Complementation tests between mutations in the phosphatespecific transport region ofEscherichia coli

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Abstract

Complementation between mutants impaired in inorganic phosphate (Pi) transport via the phosphate-specific transport (PST) system was studied. For that purpose the transport of Pi via the alternative Pi transport (PIT) system was bioenergetically arrested. Complementation was found betweenpstB andphoT mutations, whereas each of these mutations failed to complement with aphoS mutation. The data obtained confirm previous studies in which the inducibility of alkaline phosphatase was used to determine complementation and indicated a polar effect of thephoS mutation onpstB andphoT.

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Authors and Affiliations

  1. Department of Microbiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel

    Ruth Brucker & Ilan Friedberg

  2. Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel

    Ruth Levitz & Ezra Yagil

Authors
  1. Ruth Brucker
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  2. Ruth Levitz
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  3. Ezra Yagil
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  4. Ilan Friedberg
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Brucker, R., Levitz, R., Yagil, E. et al. Complementation tests between mutations in the phosphatespecific transport region ofEscherichia coli . Current Microbiology 10, 303–307 (1984). https://doi.org/10.1007/BF01577145

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