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Molecular characterization of specific heat shock proteins inBacillus subtilis

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Abstract

Heat shock inBacillus subtilis may induce as many as 66 proteins after temperature upshift from 37° to 48°C. Four induced proteins were analyzed by microsequencing techniques. These were identified as the homologues for GroEL, DnaK, enolase, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which are heat shock proteins in other systems. The identities of GroEL and DnaK were confirmed additionally by Western blot analysis. As a control, a protein whose synthesis was repressed approximately threefold by heat shock was identified by microsequencing as flagellin.

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Authors and Affiliations

  1. Center for Neurobiology and Behavior, Columbia University, New York, New York, USA

    Timothy E. Kennedy

  2. Department of Microbiology and Immunology, School of Medicine, University of Louisville, 40292, Louisville, KY, USA

    Brian S. Miller &  Uldis N. Streips

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  1. Brian S. Miller
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  2. Timothy E. Kennedy
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  3. Uldis N. Streips
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Miller, B.S., Kennedy, T.E. & Streips, U.N. Molecular characterization of specific heat shock proteins inBacillus subtilis . Current Microbiology 22, 231–236 (1991). https://doi.org/10.1007/BF02092314

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  • DOI: https://doi.org/10.1007/BF02092314

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