Abstract
An advanced analytical chemistry laboratory experiment involving the simultaneous determination of the antibiotics sulfamethoxazole and trimethoprime in commercial tablets is described. It involves the following steps: (1) preparation of nine calibration mixtures and the recording of their absorption spectra in the region 200–320 nm, (2) dissolution of the tablet contents and the recording of spectrophotometrics data, and (3) processing the data with the multivariate calibration technique of principal component regression (PCR). The theory of PCR is discussed, and a Visual Basic program is made available for data processing. The latter program allows students to obtain and save relevant statistical information (root-mean-square deviation, correlation coefficient, and relative error of prediction), as well as calibrate spectral factors and spectral residuals for each test sample. This program helps to illustrate the PCR technique in detail. The reagents used are of low cost and nontoxic; the experiment is simple and provides students with insight into a the real practice of integrating chemistry, instrumentation and computer techniques.
Similar content being viewed by others
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Ribone, M.E., Pagani, A.P., Goicoechea, H.C. et al. Simultaneous Determination of Two Antibiotics in Tablets by Spectrophotometry and Principal Component Regression (PCR) Analysis. An Advanced Undergraduate Experiment Involving Chemometrics. Chem. Educator 5, 236–241 (2000). https://doi.org/10.1007/s00897000417a
Issue Date:
DOI: https://doi.org/10.1007/s00897000417a