Abstract
Using the whole pancreas transplantation model, we investigated the endocrine function of the isografted pancreatic A, B and D cells. The release of insulin, glucagon and somatostatin by the pancreatic grafts were examined and compared with those in normal rats with or without syngenic pancreas transplanation, usingin vivo andin vitro systems. These studies demonstrate that the grafted pancreas maintained normal insulin, glucagon and somatostatin secretion after transplanation. This pancreatic graft may serve as a suitable experimental model for the studies on therapy for diabetes millitus.
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Nozawa, M.: Effects of pancreas transplantation on renal changes in streptozotocin diabetic rats. J. Japan Diab. 17: 522–524, 1974.
Kadowaki, S., Seino, Y., Taminato, T., Goto, Y., Inoue, Y., Mori, K., Chiba, T., Imura, H., Nishimoto, M., Satake, Y., and Nozawa, M.: Alteration of plasma insulin and glucagon responses to arginine in diabetic rats with pancreas isografts. J. Japan Diab. Soc. 21: 643–649, 1978.
Lee, S., Tung, K.S.K., Koopman, H., Chandler, J.G., and Orloff, M.J.: Pancreaticoduodenal transplantation in the rat. Transplantation 13: 421–425, 1972.
Grodsky, G.M., Batts, A.A., Bennett, L.L., Vcella, C., McWilliams, N.B., and Smith, D.F.: Effects of carbohydrates on secretion of insulin from isolated rat pancreas. Am. J. Physiol. 205: 638–644, 1963.
Desbuquois, B., and Aurbach, G.D.: Use of polyethleneglycol to separate free and antibody-bound peptide hormones in radioimmunoassays. J. Clin. Endocrinol. Metab. 33: 732–738, 1971.
Sakurai, H., Kuzuya, H., Kurahachi, H., Fukase, M., and Imura, H.: A sensitive radioimmunoassay for glucagon using talc method. Japan J. Nucl. Med. 10: 135–136, 1973.
Chiba, T., Sieno, Y., Goto, Y., Kadowaki, S., Taminato, T., Abe, H., Kato, T., Matsukura, S., Nozawa, M., and Imura, H.: Somatostatin release from isolated perfused rat stomach. Biochem. Biophys. Commun. 82: 731–737, 1978.
Weil, R., Nozawa, M., Koss, M., Weber, C., Reemtsman, K., and McIntosh, R.: Pancreatic transplantation in diabetic rats: Renal function, morphology, ultrastructure, and immunohistology. Surgery 78: 142–148, 1975.
Mauer, S.M., Sutherland, D.E.R., Steffes, M.W., Leonard, R.J., Najarian, J.S., Michael, A.F., and Brown, D.M.: Pancreatic transplantation: Effects on the glomerular lesions of experimental diabetes in the rat. Diabetes 23: 748–753, 1974.
Barker, C.F.: Transplantation of the islets of Langerhans and the histocompatibility of endocrine tissue. Diabetes 24: 766–775, 1975.
Lee, C.S., Mauer, S.M., Brown D.M., Sutherland, D.E.R., Michael, A.F., and Najarian, J.S.: Renal transplantation in diabetes mellitus in rats. J. Exp. Med. 139: 793–800, 1974.
Brynger, H., Blues, G., and Lernmark, A.: Glucagon and insulin release from the allografted canine pancreas. Eur. Surg. Res. 7: 170–180, 1975.
Orloff, M.J., Lee, S., Charters, A.C., Grambort, D.E., Storck, G., and Knox, D.: Long-term studies of pancreas transplantation in experimental diabetes mellitus. Ann. Surg. 182: 198–206, 1975.
Charles, A., Imagawa, W., Grodsky, G.: In vitro insulin release from the isolated perfused liver containing islet isografts. Diabetes 24: 419–423, 1975.
Luft, R., Efendic, S., Hökfelt, T., Johansson, O., and Arimura, A.: Immunohistochemical evidence from the localization of somatostatin-like immunoreactivity in a cell population of the pancreatic islets. Med. Biol. (Helsinki) 52: 428–430, 1976.
Patton, G.S., Ipp, E., Dobbs, R.E., Vale, W., Orci, L., and Unger, R.H.: Response of pancreatic immunoreactive somatostatin to arginine. Life Sci. 19: 1957–1960, 1976.
Ipp, E., Dobbs, R., Arimura, A., Vale, W., Harris, V., and Unger, R.H.: Release of immunoreactive somatostatin from the pancreas in response to glucose, amino acids, pancreozymincholecystokinin and tolbutamide. J. Clin. Invest. 60: 760–765, 1977.
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Nozawa, M., Kadowaki, S., Taminato, T. et al. Endocrine function of pancreas transplant: Insulin, glucagon and somatostatin release from rat pancreatic isograft. The Japanese Journal of Surgery 12, 296–301 (1982). https://doi.org/10.1007/BF02469564
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DOI: https://doi.org/10.1007/BF02469564