Abstract
Levels of the granulocyte colony-stimulating factor (G-CSF) were determined in the plasma and resected lung tissue from patients who underwent pulmonary resection. Moreover, the effect of recombinant human (rh) G-CSF on the permeability of pulmonary endothelium and on liquid clearance from the alveolar spaces was investigated in rabbits. The plasma levels of G-CSF increased from 30 pg/ml preoperatively to 409 ± 236 pg/ml 3 h postoperatively (P < 0.05), while the levels of G-CSF in the resected lung tissue were increased in the alveolar fluid, to 1,834 ± 1,054 pg/ml, and in the pulmonary blood, to 5,466 ± 2,019 pg/ml. It was found that rh G-CSF 25 μg administered into the subcutaneous tissue of rabbits increased extravascular lung water to 3.45 ± 0.26 vs 2.98 ± 0.20 in control experiments (P < 0.05); however, rhG-CSF 0.75 pg/kg administered into the alveloar spaces did not affect liquid clearance from the alveolar spaces. The findings of this study led us to conclude that G-CSF is synthesized in the human lung and increases the permeability of pulmonary endothelium, but not liquid clearance across the alveolar epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Layton JE (1992) Granulocyte colony-stimulating factor: structure, function, and physiology. Growth Factors 6:179–186
Nagata S, Tsuchiya M, Asano S, Kaziro Y, Yamazaki T, Yamamoto O, Hirata Y, Kubota N, Oheda M, Nomura H, Ono M (1986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. Nature 319:415–418
Souza LM, Boone TC, Gabrilove J, Lai PH, Zsebo KM, Murdock DC, Chazin VR, Bruszewski J, Lu H, Chen KK, Barendt J, Platzer E, Moore MAS, Mertelsmann R, Welte K (1986) Recombinant human granulocyte colony-stimulating factor: Effects on normal and leukemic myeloid cells. Science 232:61–65
Kawakami M, Tsutsumi H, Kumakawa T, Abe H, Hirai M, Kurosawa S, Mori M, Fukushima M (1990) Levels of serum granulocyte colony-stimulating factor in patients with infections. Blood 76:1962–1964
Watari K, Asano S, Shirafuji N, Kodo H, Ozawa K, Takaku F, Kamachi S (1989) Serum granulocyte colony-stimulating factor levels in healthy volunteers and patients with various disorders as estimated by enzyme immunoassay. Blood 73:117–122
Sakuma T, Kubo H, Tanita T, Koike K, Fujimura S (1992) Migration of neutrophils from the lung into the pleural space after lung resection in humans and rabbits. Chest 102:812–818
Motojima H, Kobayashi T, Shimane M, Kamachi S, Fukushima M (1989) Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF). J Immunol Methods 118:187–192
Smedira N, Gates L, Hastings R, Jayr C, Sakuma T, Pittet JF, Matthay MA (1991) Alveolar and lung liquid clearance in anesthetized rabbits. J Appl Physiol 70:1827–1835
Selinger SL, Bland RD, Demling RH, Staub NC (1975) Distribution volumes of [131I]albumin, [14C]sucrose, and 3601 in sheep lung. J Appl Physiol 39:773–779
Shirafuji N, Asano S, Matsuda S, Watari K, Takaku F, Nagata S (1990) A new bioassay for human granulocyte colonystimulating factor (hG-CSF) using murine myeloblastic NFS-60 cells as targets and estimation of its levels in sera from normal healthy persons and patients with infections and hematological disorders. Exp Hematol 17:116–119
Vellenga E, Rambaldi A, Ernst TJ, Ostapovicz D, Griffin JD (1988) Independent regulation of M-CSF and G-CSF gene expression in human monocytes. Blood 71:1529–1532
Broudy VC, Kaushansky K, Harlan JM, Adamson JW (1987) Interleukin 1 stimulates human endothelial cell's to produce granulocyte-macrophage colony-stimulating factor and granulocyte colony-stimulating factor. J Immunol 139:464–468
Seelentag WK, Mermod J-J, Montesano R, Vassalli P (1987) Additive effects of interleukin 1 and tumour necrosis factor-α on the accumulation of the three granulocyte and macrophage colony-stimulating factor mRNAs in human endothelial cells. EMBO J 6:2261–2265
Zsebo KM, Yuschenkoff VN, Schiffer S, Chang D, McCall E, Dinarello CA, Brown MA, Altrock B, Bagby GC (1988) Vascular endothelial cells and granulopoiesis: Interleukin-1 stimulates release of G-CSF and GM-CSF. Blood 71:99–103
Segal GM, Bagby GC Jr (1988) Vascular endothelial cells and hematopoietic regulation. lnt J Cell Clon 6:306–312
Lindemann A, Riedel D, Oster W, Ziegler-Heitbrock HWL, Mertelsmann R, Herrmann F (1989) Granulocyte-macrophage colony-stimulating factor induces cytokine secretion by human polymorhonuclear leukocytes. J Clin Invest 83:1308–1312
Shasby MD, Vanbenthuysen KM, Tate RM, Shasby SS, McMurtry I, Repine JE (1982) Granulocytes mediate acute edematous lung injury in rabbits and in isolated rabbit lungs perfused with phorbol myristate acetate: role of oxygen radicals. Am Rev Respir Dis 125:443–447
Haslett C, Worthen SG, Giclas PC, Morrison DC, Henson JE, Henson PM (1987) The pulmonary vascular sequestration of neutrophils in endotoxemia is initiated by an effect of endotoxin on the neutrophil in the rabbit. Am Rev Respir Dis 136:9–18
Flick MR, Perel A, Staub NC (1981) Leukocytes are required for increased lung microvascular permeability after microembolization in sheep. Circ Res 48:344–351
Meyrick B, Brigham KL (1983) Acute effects ofEscherichia coli endotoxin on the pulmonary microcirculation of anesthetized sheep. Lab Invest 48:458–470
Weiland JE, Davis BW, Holter JF, Mohammed JR, Dorinsky PM, Gadek JE (1986) Lung neutrophils in the adult respiratory distress syndrome. Am Rev Respir Dis 133:218–225
Kitagawa S, Yuo A, Souza LM, Saito M, Miura Y, Takaku F (1987) Recombinant human granulocyte colony-stimulating factor enhances superoxide release in human granulocytes stimulated by the chemotactic peptide. Biochem Biophys Res Commun 144:1143–1146
Kanazawa M, Ishizaka A, Hasegawa N, Suzuki Y, Yokoyama T (1992) Granulocyte colony-stimulating factor does not enhance endotoxin-induced acute lung injury in guinea pigs. Am Rev Respir Dis 145:1030–1035
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sakuma, T., Nakada, T., Nishimura, T. et al. The granulocyte colony-stimulating factor produced in the human lung and its effect on liquid movement in the rabbit lung. Surg Today 24, 1050–1055 (1994). https://doi.org/10.1007/BF01367455
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01367455