Summary
Using the “canine model of complete ischemic brain regulated with a perfusion method” in which it is possible to control the degree of blood flow to a cerebral hemisphere via a perfusion pump, the effects of mannitol (which acts as a free radical scavenger) and fluorcarbon emulsion (FC) (which has 0.1 μm of average particle size and a high oxygen-carrying capacity) on cerebral ischaemia were investigated. After pretreatment with the drugs, blood flow was reduced via the pump to 1/10 the normal state and 1 hour later, return to a normal state allowed. Subsequent changes in electrical activity were observed and the effects of the drugs evaluated.
In the control group, no recovery of electrical activity was seen, but in the animals treated with either mannitol or FC, incomplete, yet distinct recovery was apparent. In the animals administered mannitol together with FC, however, marked recovery was evident.
These experimental results indicate that the combined administration of mannitol and FC is effective in protecting the brain from cerebral ischaemia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brouwn, F. D., Hanlon, K., Mullan, S., Treatment of aneurysmal hemiplegia with dopamine and mannitol. J. Neurosurg.49 (1978), 525–529.
Butterfield, J. D., McGraw, C. P., Free radical pathology. Stroke9 (1978), 443–445.
Clark, L. C., Jr., Gollan, F., Survival of mammals breathing organic liquids equilibrated with oxygen at atmospheric pressure. Science152 (1966), 1755–1766.
Demopoulos, H. B., Flamm, E., Seligman, M.,et al., Molecular pathology of lipids in CNS membranes. In: Oxygen and physiological function (Jöbsis, F. F., ed.), pp. 491–508. Dallas: Professional Information Library. 1977.
Flamm, E. S., Demopoulos, H. B., Seligman, M. L.,et al., Free radicals in cerebral ischemia. Stroke9 (1978), 445–447.
Gaudet, R. J., Levine, L., Transient cerebral ischemia and brain postaglandins. Biochem. Biophys. Res. Commun.86 (1979), 893–901.
Geyer, R. P., Monroe, R. G., Taylor, K., Survival of rats perfused with a fluorocarbon-detergent preparation. In: Organ perfusion and preservation (Norman, J. C., ed.), pp. 85–96. New York: Appleton, Century Crafts. 1968.
Goluboff, B., Shenkin, H. A., Haft, H., The effects of mannitol and urea on cerebral hemodynamics and cerebrospmal fluid pressure. Neurology14 (1964), 891–898.
Johnston, I. H., Harper, A. M., The effect of mannitol on cerebral blood flow. An experimental study. J. Neurosurg.38 (1973), 461–471.
Kayama, T., Mizoi, K., Suzuki, J., A canine model of complete ischemic brain regulated with a perfusion method. Surg. Neurol. (accepted).
Kayama, T., Mizoi, K., Suzuki, J., Induction and propagation of lipid peroxidation in complete ischemic brain and its prevention. (In preparation.)
Little, J. R., Modification of acute focal ischemia by treatment with mannitol. Stroke9 (1978), 4–9.
Majewska, M. D., Strosznaj, J., Lazdrewicz, J., Effect of ischemic anoxia and barbiturate anesthesia on free radical oxidation on mitochondrial phospholipids. Brain Res.158 (1978), 423–434.
Meyer, J. S., Fukuuchi, Y., Shimazu, K., Ohuchi, T., Ericsson, A. D., Effect of intravenous infusion of grycerol on hemispheric blood flow and metabolism in patients with acute cerebral infarction. Stroke3 (1972), 168–180.
Rosemblum, W. I., Fluorocarbon emulsions and cerebral microcirculation. Fed. Proc.34 (1975), 1493–1498.
Seki, H., Ogawa, A., Mizoi, K., Yoshimoto, T., Suzuki, J., The effect of mannitol on cerebral circulation, Experimental focal cerebral infarction. 5th Asian-Australian Congress of Neurological Surgery, Manila, Philippine, 1979.
Sloviter, H. A., Kamimoto, T., Erythrocyte substitute for perfusion of brain. Nature216 (1967), 458–460.
Suzuki, J., Method of prolongation of temporary stopping of the cerebral blood flow. Presidential address. 33rd Annual Meeting of the Japan Neurosurgical Society. Sendai, Japan, October 22, 1974.
Suzuki, J., Yoshimoto, T., The effect of mannitol in prolongation of permissible occlusion time of cerebral artery—Clinical data of aneurysm surgery. Neurosurg. Rev.1 (1979), 13–19.
Suzuki, J., Yoshimoto, T., Tanaka, S., Sakamoto, T., Production of various models of cerebral infarction in the dog by means of occlusion of intracranial trunk arteries. Stroke11 (1980), 337–341.
Watanabe, T., Yoshimoto, T., Suzuki, J., Ultrastructural observation on the infarctic sequential changes in the cerebral tissue of dog-3, Effect of mannitol in preventing development of cerebral infarction. Neurol. Res. (submitted), 1979.
Yoshimoto, T., Suzuki, J., Prevention of experimental cerebral infarction by mannitol and clinical data of aneurysm surgery by temporary occlusion of brain artery under mannitol administration. 4th Asian and Australian Congress of Neurological Surgery, Bangkok, Thailand, 1975.
Yoshimoto, T., Suzuki, J., Intracranial defenitive aneurysm surgery under normothermia and normotension, utilizing temporary occlusion of brain artery and preoperative mannitol administration. Neurol. Surg. (Tokyo)4 (1976), 775–783.
Yoshimoto, T., Sakamoto, T., Suzuki, J., Experimental cerebral infarction, Part 1. Production of thalamus infarction in dogs. Stroke9 (1978), 211–214.
Yoshimoto, T., Sakamoto, T., Watanabe, T., Tanaka, S., Suzuki, J., Experimental cerebral infarction. Part 3. Protective effect of mannitol in thalamus infarction in dogs. Stroke9 (1978), 217–218.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mizoi, K., Yoshimoto, T. & Suzuki, J. Experimental study of new cerebral protective substances — functional recovery of severe, incomplete ischaemic brain lesions pretreated with mannitol and fluorocarbon emulsion. Acta neurochir 56, 157–166 (1981). https://doi.org/10.1007/BF01407227
Issue Date:
DOI: https://doi.org/10.1007/BF01407227