Skip to main content
SpringerLink
Log in

Lack of permanent nigrostriatal dopamine deficit following 6-hydroxydopamine injection into the rat striatum

  • Short Communication
  • Published:
Journal of Neural Transmission Aims and scope Submit manuscript

Summary

The lesion caused by a single 6-hydroxydopamine injection into rat striatum was evaluated. In vivo positron emission tomography using a dopamine reuptake tracer revealed no consistent reduction in striatal dopamine transporter. Amphetamine rotation test was negative up to 18 weeks. A 21% reduction in striatal dopamine seen at 11 weeks was not detectable at 18 weeks. Tyrosine hydroxylase-positive neurone counts showed no decline in substantia nigra. Our results suggest that this lesion may be subject to compensation and therefore should be used with caution in studies on neuroprotective treatments of Parkinson' disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Blanchard V, Anglade P, Dziewczapolski G, Savasta M, Agid A, Raisman-Vozari R (1996) Dopaminergic sprouting in the striatum after partial lesion of the substantia nigra. Brain Res 709: 319–325

    Google Scholar 

  • Bloomfield PM, Rajeswaran S, Spinks TJ, Hume SP, Myers R, Ashworth S, Clifford KM, Jones WF, Byars LG, Young J, Andreaco M, Williams CW, Lammertsma AA, Jones T (1995) The design and physical characteristics of a small animal positron emission tomograph. Phys Med Biol 40: 1105–1126

    Google Scholar 

  • Blunt SB, Jenner P, Marsden CD (1991) The effect of L-DOPA and carbidopa treatment on the survival of rat fetal DA grafts assessed by tyrosine hydroxylase immunohistochemistry and3H-mazindol autoradiography. Neuroscience 43: 95–110

    Google Scholar 

  • Brodie ME, Opacka J (1985) Dissociation between circling behaviour and striatal dopamine activity following unilateral deltamethrin administration to rats. NS Arch Pharmacol 331: 341–346

    Google Scholar 

  • Hefti F, Melamed E, Sahakian B, Wurtman RJ (1979) Circling behaviour in rats with partial, unilateral nigro-striatal lesions: effect of amphetamine, apomorphine, and DOPA. Pharmacol Biochem Behav 12: 185–188

    Google Scholar 

  • Hume SP, Luthra SK, Brown DJ, Opacka-Juffry J, Osman S, Ashworth S, Myers R, Brady F, Carroll FI, Kuhar MJ, Brooks DJ (1996) Evaluation of [11C]RTI-121 as a selective radioligand for PET studies of the dopamine transporter. Nucl Med Biol 23: 377–384

    Google Scholar 

  • Morrish PK, Sawle GV, Brooks DJ (1996) An [18F]dopa-PET and clinical study of the rate of progression in Parkinson's disease. Brain 119: 585–591

    Google Scholar 

  • Myers R, Hume SP, Ashworth S, Lammertsma AA, Bloomfield PM, Rajeswaran S, Jones T (1995) Quantification of dopamine receptors and transporter in rat striatum using a small animal PET scanner. Neuroimage 2: S8

    Google Scholar 

  • Opacka-Juffry J, Ashworth S, Hume S, Martin D, Brooks D, Blunt SB (1995) GDNF protects against 6-OHDA nigrostriatal lesion: in vivo study with microdialysis and PET. NeuroReport 7: 348–352

    Google Scholar 

  • Pellegrino LJ, Pellegrino AS, Cushman AJ (1979) A stereotaxic atlas of the rat brain, 2nd edn. Plenum Press, New York

    Google Scholar 

  • Przedborski S, Levivier M, Jiang H, Ferreira M, Jackson-Lewis V, Donaldson D, Togasaki D (1994) Partial lesions of the dopaminergic nigrostriatal pathway induced by intrastriatal injection of 6-OHDA: rotational behaviour, quantitative autoradiography and histology. Mov Disord 9 [Suppl] 1: P444

    Google Scholar 

  • Przedborski S, Levivier M, Jiang H, Ferreira M, Jackson-Lewis V, Donaldson D, Togasaki D (1995) Dose-dependent lesions of the dopaminergic nigrostriatal pathway induced by intrastriatal injection of 6-OHDA. Neuroscience 67: 631–47

    Google Scholar 

  • Robinson TE, Whishaw IQ (1987) Normalization of extracellular dopamine in striatum following recovery from a partial unilateral 6-OHDA lesion of the substantia nigra: a microdialysis study in freely moving rats. Brain Res 450: 209–224

    Google Scholar 

  • Sauer H, Oertel WH (1994) Progressive degeneration of nigrostriatal dopamine neurons following intrastriatal terminal lesions with 6-hydroxydopamine: a combined retrograde tracing and immunocytochemical study in the rat. Neuroscience 59: 401–415

    Google Scholar 

Download references

Author information

Author notes

  1. S. B. Blunt

    Present address: MRC Cyclotron Unit, MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, London

Authors and Affiliations

  1. MRC Cyclotron Unit, MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, London

    J. Opacka-Juffry, S. Ashworth, A. M. Sullivan & R. B. Banati

  2. Institute of Neurology, Queen Square, London, UK

    R. B. Banati

Authors
  1. J. Opacka-Juffry
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Ashworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. M. Sullivan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. B. Banati
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. B. Blunt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Opacka-Juffry, J., Ashworth, S., Sullivan, A.M. et al. Lack of permanent nigrostriatal dopamine deficit following 6-hydroxydopamine injection into the rat striatum. J. Neural Transmission 103, 1429–1434 (1996). https://doi.org/10.1007/BF01271256

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01271256

Keywords

Search

Navigation