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A study on postmortem stability of vasopressin messenger RNA in rat brain compared with those in total RNA and ribosomal RNA

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Summary

Postmortem stability of arginine-vasopressin (AVP) messenger RNA (mRNA) in the rat brain was studied comparing changes with those in the recovered amounts of total RNA and ribosomal RNA (rRNA). The amount of AVP mRNA and rRNA showed a decrease with increasing time interval after death (postmortem time), whereas the amount of total RNA did not alter with postmortem time. The half-life of AVP mRNA in the rat postmortem seemed to be approximately 16 hrs. The analysis of the ratio of AVP mRNA to 18S-rRNA suggested that AVP mRNA was degraded postmortem more rapidly than rRNA. These results suggest that autopsied human brains should be used for AVP mRNA study within a short postmortem time.

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References

  • Arai H, Noguchi I, Sagi N, Moroji T, Iizuka R (1989) A study of in situ hybridization histochemistry on postmortem changes in vasopressin mRNA in rat brain. Neurosci Lett 103: 127–132

    PubMed  Google Scholar 

  • Baldino Jr F, Ruth JL, Davis LG (1989) Nonradioactive detection of vasopressin mRNA with in situ hybridization histchemistry. Exp Neurol 104: 200–207

    PubMed  Google Scholar 

  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299

    PubMed  Google Scholar 

  • Dabis LG, Arentzen R, Reid JM, Manning RW, Lawrense KL, Baldino Jr F (1986) Glucocorticoid sensitivity of vasopressin mRNA levels in the paraventricular nucleus of the rat. Proc Natl Acad Sci USA 83: 1145–1149

    PubMed  Google Scholar 

  • Dani C, Blanchard JM, Piechaczyk M, El Sabouty S, Marty L, Jeanteur P (1984) Extreme instability of myc mRNA in normal and transformed human cells. Proc Natl Acad Sci USA 81: 7046–7050

    PubMed  Google Scholar 

  • Glowinski J, Iversen LL (1966) Resional studies of catecholamines in the rat brain-1. J Neurochem 13: 655–669

    PubMed  Google Scholar 

  • Johnson SA, Morgan DG, Finch CE (1986) Extensive postmortem stability of RNA from rat and human brain. J Neurosci Res 16: 267–280

    PubMed  Google Scholar 

  • Marotta CA, Brown BA, Strocchi P, Bird ED, Gilbert JM (1981) In vitro synthesis of human brain proteins including tubulin and actin by purified postmortem polysomes. J Neurochem 36: 966–975

    PubMed  Google Scholar 

  • Morrison MR, Griffin WS (1981) The isolation and in vitro translation of undegraded messenger RNAs from human postmortem brains. Anal Biochem 113: 318–324

    PubMed  Google Scholar 

  • Perrett CW, Marchbanks RM, Whatley SA (1988) Characterization of messenger RNA extracted postmortem from the brains of schizophrenic, depressed and control subjects. J Neurol Neurosurg Psychiatry 51: 325–331

    PubMed  Google Scholar 

  • Revkees SA, Chaar MR, Hanley DF, Maxwell M, Reppert SM, Uhl GR (1989) Localization and regulation of vasopressin mRNA in human neurons. Synapse 3: 246–254

    PubMed  Google Scholar 

  • Ross J, Sullivan T (1985) Half-lives of beta and gamma globin messenger RNAs and of protein synthetic capacity in cultured human reticulocytes. Blood 66: 1149–1154

    PubMed  Google Scholar 

  • Sajdel-Sulkowska E, Coughlin JF, Marotta CA (1983) In vitro synthesis of polypeptides of moderately large size by poly(A)-containing messenger RNA from postmortem human brain and mouse brain. J Neurochem 40: 670–680

    PubMed  Google Scholar 

  • Schröder HC, Zahn RK, Dose K, Müller WEG (1980) Purification and characterization of poly(A)-specific exoribonuclease from calf thymus. J Biol Chem 255: 4535–4538

    PubMed  Google Scholar 

  • Sherman TG, McKelvy JF, Watson SJ (1986) Vasopressin mRNA regulation in individual hypothalamic nuclei: a northern and in situ hybridization analysis. J Neurosci 6: 1685–1694

    PubMed  Google Scholar 

  • Spokes EGS, Koch DJ (1978) Post-mortem stability of dopamine, glutamate decarboxylase and choline acetyltransferase in the mouse brain under conditions simulating the handling human autopsy material. J Neurochem 31: 381–383

    PubMed  Google Scholar 

  • Taylor GR, Carter GI, Crow TJ, Johnson JA, Fairbairn AF, Perry EK, Perry RH (1986) Recovery and measurement of specific RNA species from postmortem brain tissue: a general reduction in Alzheimer's disease detected by molecular hybridization. Exp Mol Pathol 44: 111–116

    PubMed  Google Scholar 

  • Wreschner DH, Rechavi G (1988) Differential mRNA stability to reticulocyte ribonucleases correlated with 3′ non-coding (U)nA sequences. Eur J Biochem 172: 333–340

    PubMed  Google Scholar 

  • Yoshikawa K, Aizawa T (1988) Transient increases and individual variations in preprocholecystokinin gene expression in rat telencephalic regions during postnatal development: a quantitative comparison between preprocholecystokinin in mRNA and preproenkephalin mRNA. Mol Brain Res 4: 87–96

    Google Scholar 

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  1. I. Noguchi

    Present address: Department of Psychiatry, Juntendo University School of Medicine, Tokyo, Japan

Authors and Affiliations

  1. Department of Psychiatry, Juntendo University School of Medicine, Tokyo, Japan

    H. Arai & R. Iizuka

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  1. I. Noguchi
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  2. H. Arai
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  3. R. Iizuka
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Noguchi, I., Arai, H. & Iizuka, R. A study on postmortem stability of vasopressin messenger RNA in rat brain compared with those in total RNA and ribosomal RNA. J. Neural Transmission 83, 171–178 (1991). https://doi.org/10.1007/BF01253387

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  • DOI: https://doi.org/10.1007/BF01253387

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