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Biosynthesis and morphogenesis of group C rotavirus in swine testicular cells

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Summary

Polypeptide synthesis and morphogenesis of a group C rotavirus (AmC-1) adapted to a continuous swine testicular cell line was examined. SDS-PAGE analysis of35S methionine labeled infected cell lysates revealed 9 viral polypeptides (122, 98, 79, 78, 43, 41, 35, 24, and 20 kD). Viral polypeptide synthesis appeared to be maximal at 7–10 h post infection. Purified group C virus grown in the presence of trypsin was found to contain seven structural polypeptides (122, 98, 79, 53, 43, 41, and 30 kD) by protein blotting and five polypeptides (98, 79, 78, 43, and 41 kD) by immunoprecipitation with a hyperimmune rabbit antisera. Tunicamycin treatment, Concanavalin A binding, protein blotting, endo-H treatment and2,6H-mannose labeling suggested that group C rotavirus contains one structural glycoprotein (41 kD) with a corresponding precursor mol. wt. of 37 kD and one not previously identified non-structural glycoprotein (24 kD) with a corresponding precursor mol. wt. of ⩽20 kD. Electron microscopy of infected swine testicular cells revealed an assembly process for group C rotavirus similar to group A, with single-shelled particles budding through the rough endoplasmic reticulum with concomitant acquisition of a transient membrane.

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Authors and Affiliations

  1. Department of Virology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden

    M. Nilsson & L. Svensson

  2. Department of Virology, University of Helsinki, Finland

    C. H. von Bonsdorff

Authors
  1. M. Nilsson
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  2. C. H. von Bonsdorff
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  3. L. Svensson
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Nilsson, M., von Bonsdorff, C.H. & Svensson, L. Biosynthesis and morphogenesis of group C rotavirus in swine testicular cells. Archives of Virology 133, 21–37 (1993). https://doi.org/10.1007/BF01309741

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  • DOI: https://doi.org/10.1007/BF01309741

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