Summary
We describe methods for the preparation of collagen and gelatin samples to detect possible prion contaminants using Western blotting of a major component of prions, PrPSc. A commercially available collagen solution containing 2% athero-collagen was spiked with rodent adapted scrapie prion and used as the prion-contaminating collagen. The methods developed center on the enzymatic reduction of the collagen solution viscosity with protease treatments and on the concentration of the prion from the protease-digests with polyethylene glycol-#6000 and NaCl. Recovery of the spiked prion as a partially protease-resistant core fragment of PrPSc fluctuated from 30% to 46% of the input amount.
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Received June 19, 1998 Accepted August 5, 1998
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Nemoto, T., Horiuchi, M., Ishiguro, N. et al. Detection methods of possible prion contaminants in collagen and gelatin. Arch. Virol. 144, 177–184 (1999). https://doi.org/10.1007/s007050050494
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DOI: https://doi.org/10.1007/s007050050494