Summary
Monoclonal antibodies against 3 different glycoproteins of bovine herpesvirus type 1 (BHV-1) involved in virus neutralization were used in indirect immunofluorescence (IIF) tests to characterize the appearance and transport to the plasma membrane of virus antigens in the infected cells.
Antibodies against gp 117 and gp 71 glycoproteins first showed pronounced ring-like nuclear fluorescence at 4 hours post-infection (PI), followed by staining of the perinuclear region, presumably the Golgi apparatus. In contrast, antibody against gp 87 produced staining in cell-to-cell junctional areas at 3 hours PI before any staining close to the nucleus.
The expression of the 3 glycoproteins at the surface of the infected cells was confirmed by the use of monoclonal antibodies having neutralizing activity, but not by non-neutralizing antibodies against gp 117 and gp 71. Non-neutralizing antibody against gp 87 detected the surface fluorescence only in those cells showing marked degeneration.
Inhibition of glycosylation of the viral glycoproteins with tunicamycin (TM) was followed by interference with transport of gp 117 and gp 87 to the plasma membrane. On the other hand, gp 71 was incorporated into the plasma membrane despite the lack of N-linked glycosylation.
Similar content being viewed by others
References
Bolton DC, Zee YC, Ardans AA (1983) Identification of envelope and nucleocapsid proteins of infectious bovine rhinotracheitis virus by SDS-polyacrylamide gel electrophoresis. Vet Microbiol 8: 57–68
Choppin PW, Scheid A (1980) The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis 2: 40–61
Collins JS, Butcher AC, Riegel CA, McGrane V, Blair CD, Teramoto YA, Wilson S (1984) Neutralizing determinants defined by monoclonal antibodies on polypeptides specified by bovine herpesvirus 1. J Virol 52: 403–409
Dubois-Dalcq M, Holmes KV, Rentier B (1984) Assembly of enveloped RNA viruses. Springer, Wien New York, pp 1–20
Fuller AO, Spear PG (1985) Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies. J Virol55: 475–482
Johnson DC, Wittels M, Spear PG (1984) Binding to cells of virosomes containing herpes simplex virus type 1 glycoproteins and evidence for fusion. J Virol52: 238–247
Kahrs SF (1977) Infectious bovine rhinotracheitis: A review and update. J Am Vet Med Assoc 171: 1055–1064
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Merz DC, Sheid A, Choppin PW (1980) Importance of antibodies of paramyxoviruses in the prevention of spread of infection. J Exp Med 151: 275–288
Misra V, Blumenthal RM, Babiuk LA (1981) Proteins specified by bovine herpesvirus 1 (infectious bovine rhinotracheitis virus). J Virol 40: 364–378
Misra V, Gilchrist JE, Weinmaster G, Qualtiere L, Van den Hurk S, Babiuk LA (1982) Herpesvirus-induced “early” glycoprotein: Characterization and possible role in immune cytolysis. J Virol 43: 1046–1054
Multi KG, Webster RG (1986) Distribution of hemagglutinin and neuraminidase on influenza virions as revealed by immunoelectron microscopy. Virology 149: 36–43
Noble AG, Lee GT-Y, Sprague R, Parish ML, Spear PG (1983) Anti-gD monoclonal antibodies inhibit cell fusion induced by herpes simplex virus type 1. Virology 129: 218–224
Norrild B, Virtanen I, Pedersen B, Pereira L (1983) Requirements for transport of HSV-1 glycoproteins to the cell surface membrane of human fibroblasts and Vero cells. Arch Virol 77: 155–166
Okazaki K, Honda E, Minetoma T, Kumagai T (1986) Mechanisms of neutralization by monoclonal antibodies to different antigenic sites on the bovine herpesvirus type 1 glycoproteins. Virology 50: 260–264
Perrin LH, Tishon AJ, Oldstone MBA (1977) Immunogenic injury in measles virus infections III. Presence and characterization of human cytotoxic lymphocytes. J Immunol 118: 282–290
Spear PG (1980) Herpesviruses. In:Blough HA, Tiffany JM (eds) Cell membranes and viral envelopes, vol 2. Academic Press, New York, pp 709–750
Takahashi A, Kohno K, Tamura G (1975) Inhibition of biosynthesis of polyisoprenol sugars in chick embryo microsomes by tunicamycin. Agric Biol Chem 39: 2089–2091
Ulug ET, Bose HR Jr (1985) Effect of tunicamycin on the develope of the cytopathic effect in sindbis virus-infected avian fibroblasts. Virology 143: 546–557
Van Drunen Little-Van den Hurk S, Babiuk LA (1985) Effect of tunicamycin and monensin on biosynthesis, transport, and maturation of bovine herpesvirus type 1 glycoproteins. Virology 143: 104–118
Van Drunen Little-Van den Hurk S, Van den Hurk JV Gilchrist JE, Misra V, Babiuk LA (1984) Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: Characterization of their biochemical and immunological properties. Virology 135: 466–479
Webster RG, Hinshaw VS, Laver WG (1982) Selection and analysis of antigenic variants of the neuraminidase of N 2 influenza viruses with monoclonal antibodies. Virology 117: 93–104
Author information
Authors and Affiliations
Additional information
With 4 Figures
Rights and permissions
About this article
Cite this article
Okazaki, K., Kawakura, H., Okada, M. et al. Intracellular localization and transport of three different bovine herpesvirus type 1 glycoproteins involved in neutralization. Archives of Virology 92, 17–26 (1987). https://doi.org/10.1007/BF01310059
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310059