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Die Reindarstellung von Anti-BOP-Antikörpern mittels Affinitätschromatographie

The purification of anti-BPO antibodies by means of affinity chromatograph

  • Organische Chemie und Biochemie
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Monatshefte für Chemie / Chemical Monthly Aims and scope Submit manuscript

Abstract

The purification of anti-BPO-antibodies and anti-BPO-IgG resp. by means of affinity chromatography is being described. The immunosorbent was prepared by direct covalent coupling of BPO groups to AH-Sepharose 4B beads. The antibodies were linked to the immunosorbent in a batch and eluted by stepwise chromatography. The hapten density of the gel was very high (3.6×10−5 mole BPO/1 g Sepharose 4B) as could be demonstrated by application of14C-penicillin-potassium. Good yields of highly purified anti-PBO-antibodies were obtained.

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Literatur

  1. S. V. Boyden, J. Exper. Med.93, 107 (1951).

    Google Scholar 

  2. W. Knapp, Persönliche Mitteilung.

  3. B. B. Levine, M. J. Fellner undV. Levytska, J. Immunol.96, 707 (1966).

    PubMed  Google Scholar 

  4. G. W. Locher, C. H. Schneider undA. L. de Weck, Z. Imm.-Forsch.141, 265 (1971).

    Google Scholar 

  5. C. Steffen, Allgemeine und experimentelle Immunologie und Immunopathologie. Stuttgart: Thieme. 1968.

    Google Scholar 

  6. H.-P. Werner, H. Stemberger, G. Wiedermann undD. Kraft, Wr. Klin. Wschr.84, 663 (1972).

    Google Scholar 

  7. G. Wiedermann, H. Stemberger, H.-P. Werner undD. Kraft, Z. Imm.-Forsch.143, 483 (1972).

    Google Scholar 

  8. G. Wiedermann, M. M. Müller, O. Förster undH. Stemberger, Internat. Arch. Allergy Appl. Immunol.49, 843 (1975).

    Google Scholar 

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Authors and Affiliations

  1. Institut für Spezifische Prophylaxe und Tropenmedizin, Institut für Virologie und Institut für Medizinische Chemie, Universität Wien, Kinderspitalgasse 15, A-1095, Wien, Österreich

    H. Stemberger, G. Wiedermann, W. Frisch-Niggemeyer & M. M. Müller

Authors
  1. H. Stemberger
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  2. G. Wiedermann
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  3. W. Frisch-Niggemeyer
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  4. M. M. Müller
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Stemberger, H., Wiedermann, G., Frisch-Niggemeyer, W. et al. Die Reindarstellung von Anti-BOP-Antikörpern mittels Affinitätschromatographie. Monatshefte für Chemie 108, 1–6 (1977). https://doi.org/10.1007/BF00900901

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  • DOI: https://doi.org/10.1007/BF00900901

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