Summary
A simple method, involving selective Triton X-100 membrane solubilization, has been developed for the isolation of nuclei from barley and tobacco protoplasts which gives a high yield of essentially pure nuclei. The isolated nuclei resembled those in leaf cells and protoplasts when the isolated nuclei were fixed for short times (2 hours, Medium II), except that their chromatin appeared to be more highly condensed and barley nuclei also lacked the outer nuclear membrane. When longer times of fixation (12 hours, Medium I) were used, the isolated nuclei lacked the characteristic condensed chromatin appearance.
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Hughes, B.G., Hess, W.M. & Smith, M.A. Ultrastructure of nuclei isolated from plant protoplasts. Protoplasma 93, 267–274 (1977). https://doi.org/10.1007/BF01275658
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DOI: https://doi.org/10.1007/BF01275658