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l-Cysteine metabolism via 3-mercaptopyruvate pathway and sulfate formation in rat liver mitochondria

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Summary

We have studied the 3-mercaptopyruvate pathway (transamination pathway) ofl-cysteine metabolism in rat liver mitochondria.l-Cysteine and other substrates at 10 mM concentration were incubated with mitochondrial fraction at pH 8.4, and sulfate and thiosulfate were determined by ion chromatography. Whenl-cysteine alone was incubated, sulfate formed was 0.7µmol per mitochondria from one g of liver per 60 min. Addition of 2-oxoglutarate and GSH resulted in more than 3-fold increase in sulfate formation, and thiosulfate was formed besides sulfate. The sum (A + 2B) of sulfate (A) and thiosulfate (B) formed was approximately 7-times that withl-cysteine alone. Incubation with 3-mercaptopyruvate resulted in sulfate and thiosulfate formation, and sulfate was formed with thiosulfate. These reactions were stimulated with glutathione. Sulfate formation froml-cysteinesulfinate and 2-oxoglutarate was not enhanced by glutathione and thiosulfate was not formed. These findings indicate thatl-cysteine was metabolized and sulfate was formed through 3-mercaptopyruvate pathway in mitochondria.

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Authors and Affiliations

  1. Department of Biochemistry, Okayama University Medical School, 2-5-1 Shikatacho, 700, Okayama, Japan

    T. Ubuka, J. Ohta, W. -B. Yao, T. Abe, T. Teraoka & Y. Kurozumi

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  1. T. Ubuka
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  2. J. Ohta
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  3. W. -B. Yao
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  4. T. Abe
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  5. T. Teraoka
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Ubuka, T., Ohta, J., Yao, W.B. et al. l-Cysteine metabolism via 3-mercaptopyruvate pathway and sulfate formation in rat liver mitochondria. Amino Acids 2, 143–155 (1992). https://doi.org/10.1007/BF00806085

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