Skip to main content
SpringerLink
Log in

Localization and function of myocardial lipolysis

  • Original Contributions
  • Published:
Basic Research in Cardiology Aims and scope Submit manuscript

Summary

Mobilization of triacylglycerol stored in heart cells is accomplished by the combined action of lysosomal (acid) lipase and microsomal monoacylglycerol lipase or carboxylesterase. Non(heparin)-releasable neutral or alkaline lipase is similar to non(readily)-releasable lipoprotein lipase (LPL). The enzyme is mainly localized extracellularly. Non(readily)-releasable LPL probably represents LPL in caveola or vacuolae of vascular endothelium and/or LPL on myocardial interstitium. It contributes to the uptake of lipoprotein constituents in heart cells. Glycerol, an endproduct of lipolysis, is not a reliable marker for the net mobilization of lipid stored in heart cells. It is formed both intra- and extracellularly, and does not reflect the rate of oxidation of part of free fatty acids formed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Heiss HW, Barmeyer J, Wink K, Hell G, Cerny FJ, Keul J, Reindell H (1976) Studies of the regulation of myocardial blood flow in man. I. Training effect on blood flow and metabolism of the healthy heart at rest and during standardized heavy exercise. Basic Res Cardiol 71:658–675

    Article  PubMed  Google Scholar 

  2. Gousios A, Felts JM, Havel RJ (1963) The metabolism of serum triglyceride and free fatty acids by the myocardium. Metabolism 12:75–80

    PubMed  Google Scholar 

  3. Evans CAL (1939) Metabolism of the heart. Edinb med J 46:733–749

    Google Scholar 

  4. Denton RM, Randle PJ (1967) Concentration of glycerides and phospholipids in heart and gastrocnemius muscles. Effects of alloxan diabetes and perfusion. Biochem J 104:416–422

    PubMed  Google Scholar 

  5. Olson RE, Hoeschen RJ (1967) Utilization of endogenous lipids by the isolated perfused rat heart. Biochem J 103:796–801

    PubMed  Google Scholar 

  6. Shipp JC, Thomas JM, Crevasse L (1964) Oxidation of carbon-14-labeled endogenous lipids by isolated perfused rat heart. Science 143:371–373

    PubMed  Google Scholar 

  7. Crass MF III, McCaskill ES, Shipp JC (1969) Effect of pressure development on glucose and palmitate metabolism in perfused heart. Am J Physiol 211:1569–1576

    Google Scholar 

  8. Crass MF III, McCaskill ES, Shipp JC, Murthy VK (1971) Metabolism of endogenous lipids in cardiac muscle: effect of pressure development. Am J Physiol 220:428–435

    PubMed  Google Scholar 

  9. Kreisberg RA (1966) Effect of epinephrine on myocardial triglyceride and free fatty acid utilization. Am J Physiol 210:385–389

    PubMed  Google Scholar 

  10. Rizack MA (1961) An epinephrine-sensitive lipolytic activity in adipose tissue. J Biol Chem 236:657–662

    Google Scholar 

  11. Hülsmann WC, Stam H (1978) Intracellular origin of hormone-sensitive lipolysis in the rat. Biochem Biophys Res Commun 82:53–59

    PubMed  Google Scholar 

  12. Hülsmann WC, Stam H, Geelhoed-Mieras MM (1979) Hormone-sensitivity of lipolysis in various rat tissues; effect of chloroquine, phenothiazines, quinidine and lidocaine. In: Aihaud (ed) Obesity-Cellular and Molecular Aspects. INSERM, Paris, pp. 179–192

    Google Scholar 

  13. Hülsmann WC, Stam H, Breeman WAP (1981) Acid- and neutral lipases involved in endogenous lipolysis in small intestine and heart. Biochem Biophys Res Commun 102:440–448

    Article  PubMed  Google Scholar 

  14. Seeman P (1972) The membrane actions of anesthetics and tranquilizers. Pharmacol Reviews 24:583–665

    Google Scholar 

  15. Wang TW, Menahan LA, Lech JJ (1977) Subcellular localization of marker enzymes, lipase and triglyceride in rat heart. J Mol Cell Cardiol 9:25–38

    PubMed  Google Scholar 

  16. Hülsmann WC, Stam H (1979) On the regulation of triglyceride hydrolysis in heart. In: Hessel LW, Krans HMJ (eds) Lipoprotein metabolism and endocrine regulation. Elsevier/North Holland Biomedical Press, Amsterdampp, pp 289–297

    Google Scholar 

  17. Stam H, Geelhoed-Mieras MM, Hülsmann WC (1980) Erucic acid-induced alteration of cardiac triglyceride hydrolysis. Lipids 15:242–250

    PubMed  Google Scholar 

  18. Vles RO (1975) Nutritional aspects of rapeseed oil. In: Vergroesen AJ (ed) The role of fats in human nutrition. Academic Press, London New York San Francisco, pp 431–477

    Google Scholar 

  19. Yamamoto M, Drummond GI (1967) Monoglyceride-hydrolyzing activity of rat myocardium. Am J Physiol 213:1365–1370

    PubMed  Google Scholar 

  20. Biale Y, Gorin E, Shafrir E (1968) Characterization of tissue lipolytic and esterolytic activities cleaving full and partial glycerides. Biochim Biophys Acta 152:28–39

    PubMed  Google Scholar 

  21. Stam H, Hülsmann WC (1982) Effect of dietary erucic acid and the activity of lipolytic enzymes in rat heart. Biochem Int 4:83–91

    Google Scholar 

  22. Rösen P, Budde TH, Reinauer H (1981) Triglyceride lipase activity in diabetic rat heart. J Mol Cell Cardiol 13:539–550

    Article  PubMed  Google Scholar 

  23. Severson DL (1979) Characterization of triglyceride lipase activity in rat heart. J Mol Cell Cardiol 11:569–583

    PubMed  Google Scholar 

  24. Hülsmann WC, Stam H, Breeman WAP (1982) On the nature of neutral lipase in rat heart. Biochem Biophys Res Commun 108:371–378

    Article  PubMed  Google Scholar 

  25. Zimmerman ANE, Hülsmann WC (1966) Paradoxical influence of calcium ions on the permeability of the cell membranes of the isolated rat heart. Nature (Lond) 211:646–647

    Google Scholar 

  26. Zimmerman ANE, Daems W, Hülsmann WC, Snijder J, Wisse E, Durrer D (1967) Morphological changes of heart muscle caused by successive perfusion with calcium-free and calcium-containing solutions (Calcium Paradox). Cardiovasc Res 1:201–209

    Google Scholar 

  27. Jansen H, Stam H, Kalkman C, Hülsmann WC (1980) On the dual localization of lipoprotein lipase in rat heart. Studies with a modified perfusion technique. Biochem Biophys Res Commun 92:411–416

    Article  PubMed  Google Scholar 

  28. De Deckerer EAM, Ten Hoor F (1977) A modified Langendorff technique for metabolic investigations. Pflügers Archiv 370:103–105

    Article  Google Scholar 

  29. Simpson J (1979) Rapid effect of isoprenaline, glucagon, pacing and potassium arrest on postheparin lipoprotein lipase activity in the perfused rat heart. Biochem J 182:253–255

    PubMed  Google Scholar 

  30. Fielding CJ, Higgins JM (1974) Lipoprotein lipase; comparative properties of the membrane-supported and solubilized enzyme species. Biochemistry 13:4324–4330

    Article  PubMed  Google Scholar 

  31. Pedersen ME, Wolf LE, Schotz MC (1981) Hormone mediation of rat heart lipoprotein lipase activity after fat feeding. Biochim Biophys Acta 666:191–197

    PubMed  Google Scholar 

  32. Robinson DS (1970) The function of the plasma triglycerides in fatty acid transport. In: Florkin M, Stotz EH (eds) Comprehensive Biochemistry, vol. 18. Elsevier Publ Comp, Amsterdam, pp 51–116

    Google Scholar 

  33. Hülsmann WC, Stam H, Maccari F (1982) The effect of excess (acyl) carnitine on lipid metabolism in rat heart. Biochim Biophys Acta 713:39–45

    PubMed  Google Scholar 

  34. Stokke KT, Fjeld NB, Kluge TH, Skrede S (1974) Lipid composition and cholesterol esterification in lymph. Scand J Clin Lab Invest 33:199–206

    PubMed  Google Scholar 

  35. Stam H, Jansen H, Hülsmann WC (1980) Distribution of chylomicron degradation products in the isolated perfused rat heart. Biochem Biophys Res Commun 96:899–906

    Article  PubMed  Google Scholar 

  36. Hülsmann WC, Stam H (1980) Lipolysis in heart and adipose tissue; effects of glycogenolysis and uncoupling of oxidative phosphorylation. Biochem Biophys Res Commun 88:867–872

    Article  Google Scholar 

  37. Gold AM, Legrand E, Sánchez GR (1971) Inhibition of muscle phosphorylase a by 5-gluconolactone. J Biol Chem 246:5700–5706

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. W. C. Hülsmann

    Present address: Dept. of Biochemistry I, Medical Faculty, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR, Rotterdam, (The Netherlands)

Authors and Affiliations

  1. Dept. of Biochemistry I, Medical Faculty, Erasmus University Rotterdam, P. O. Box 1738, 3000 DR, Rotterdam, (The Netherlands)

    H. Stam & H. Jansen

Authors
  1. W. C. Hülsmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Stam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Jansen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hülsmann, W.C., Stam, H. & Jansen, H. Localization and function of myocardial lipolysis. Basic Res Cardiol 79, 268–273 (1984). https://doi.org/10.1007/BF01908026

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01908026

Key words

Search

Navigation