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A new method for studying the incorporation of nonesterified fatty acids into cardiac lipids by using deuterium-labelled palmitate

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Summary

A new method for measuring the incorporation of exogenous fatty acids into myocardial lipids of working rat hearts using deuterium-labelled palmitate has been developed. After perfusing isolated hearts, the fatty acid composition of triglycerides, diglyccrides, monoglycerides, cholesterol esters, and nonesterified fatty acids (NEFA) was measured by a mass fragmentographic method. Hearts perfused only with 5 mM glucose and 1 mM lactate perfusion as basic substrates showed a significant decrease of triglyceride content, while the other lipids were not found to be significantly reduced. The fatty acid composition of all lipids were not affccted. An addition of D31-palmitate complexed to albumin at a molar ratio of 5∶1, caused a dose-dependent incorporation into triglycerides and diglycerides which suggested saturation kinetics. The tissue content of nonesterified D31-palmitate was found to be linearly related to its concentration in the perfusate. It may be of note that the concentration of the other NEFA was significantly affected neither by a fatty-acid-free perfusion nor by a perfusion with D31-palmitate. A significant incorporation of the supplied fatty acid into cholesterol esters and monoglycerides could not be detected.

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Author notes

  1. J. F. Hütter

    Present address: Department of Pharmacology, Schwarz GmbH, Mittelstr. 11-13, 4019, Monheim, FRG

Authors and Affiliations

  1. Zentrum Physiologie und Pathophysiologie, Universität Göttingen Abteilung Herzstoffwechsel und Physiologie der Wiederbelebung, Göttingen, FRG

    J. F. Hütter, Ch. Schweickhardt, D. H. Hunneman, H. M. Piper & P. G. Spieckermann

Authors
  1. J. F. Hütter
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  2. Ch. Schweickhardt
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  3. D. H. Hunneman
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  4. H. M. Piper
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  5. P. G. Spieckermann
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Additional information

Supported by the Deutsche Forschungsgemeinschaft, SBF 89-Kardiologie Göttingen-

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Hütter, J.F., Schweickhardt, C., Hunneman, D.H. et al. A new method for studying the incorporation of nonesterified fatty acids into cardiac lipids by using deuterium-labelled palmitate. Basic Res Cardiol 83, 87–93 (1988). https://doi.org/10.1007/BF01907108

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  • DOI: https://doi.org/10.1007/BF01907108

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