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Local glucose utilization and local blood flow in hearts of awake rats

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Summary

Local cardiac glucose utilization and local cardiac blood flow in rat heart were measured in vivo by quantitative autoradiographic techniques with 2-[14C] deoxyglucose and [14C] iodoantipyrine, respectively. [14C]methylmethacrylate standards were calibrated for quantitative autoradiography of dried sections of heart tissue; the calibration values for heart tissue differed from those for brain by 8%, probably because of differences in self-absorption within the tissues. The lumped constant required by the deoxyglucose method was determined in isolated, perfused, working rat hearts and found to be 1.11±0.36 (mean±SD, n=21). The heart: blood partition coefficient for iodoantipyrine required by the [14C]iodoantipyrine method was measured and found to be 1.25. The results obtained in awake rats showed: 1) overall cardiac glucose utilization varied considerably among animals with a mean of 53 (left ventricle) and 30 (right ventricle) μmol/100 g/min; 2) cardiac blood flow was less variable among animals with a mean of 592 (left ventricle) and 420 (right ventricle) ml/100 g/min; 3) glucose utilization was found to be particularly high in the papillary muscle; 4) systematic gradients of glucose utilization or blood flow in the ventricular wall were not observed; 5) glucose utilization and blood flow were not closely correlated on a local level. It is concluded that autoradiographic methods are suitable for the quantification of local glucose utilization and local blood flow in the rat heart in vivo. These methods could not demonstrate transmural gradients for glucose utilization and blood flow between epi- and endocardium in awake rats.

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Abbreviations

IAP:

4-iodo-N-methyl-antipyrine

2-DG:

2-deoxy-D-glucose

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Author notes

  1. W. Kuschinsky

    Present address: Physiologisches Institut der Universität, Im Neuenheimer Feld 326, 69120, Heidelberg, Germany

Authors and Affiliations

  1. Department of Physiology, University of Heidelberg, Germany

    W. Kuschinsky & H. Schröck

  2. Laboratory of Cerebral Metabolism National Institute of Mental Health Bethesda, Maryland, USA

    W. Kuschinsky & L. Sokoloff

  3. Department of Physiology Uniformed Services University Bethesda, Maryland, USA

    R. Bünger & R. T. Mallet

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  1. W. Kuschinsky
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  2. R. Bünger
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  3. H. Schröck
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  4. R. T. Mallet
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  5. L. Sokoloff
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Kuschinsky, W., Bünger, R., Schröck, H. et al. Local glucose utilization and local blood flow in hearts of awake rats. Basic Res Cardiol 88, 233–249 (1993). https://doi.org/10.1007/BF00794996

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  • DOI: https://doi.org/10.1007/BF00794996

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