Abstract
Three different poly-L-lysine (pLys) samples and a branched polypeptide poly-[L-Lys-(DL-Ala)m] (poly-L-Lys-poly-DL-Ala, AK) were synthetized. Relative molar mass distribution, its average and the degree of polymerisation were determined by sedimentation analysis and gel permeation chromatography (GPC). The conformation of the polymers in solution was studied by circular dichroism (CD) spectroscopy at various pH values and ionic strengths. The data obtained by different methods were compared.
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References
Sela M (1966) Advan Immunology 5:29
Sela M (1974) Peptides, Polypeptides, Proteins, p 495. Wiley, New York
Szekerke M, Driscoll JS (1977) Europ J Cancer 13:529
Müller GM, Shapiro LM, Arnon R (1982) Proc Natl Acad Sci USA 79:569
Audibert F, Jolivet M, Chedid L, Arnon R, Sela M, Proc Natl Acad Sci USA 79:5042
Hudecz F, Szekerke M (1980) Coll Czech Chem Commun 45:933
Blut ER, Karlsen RH, Doty P, Hargitay BJ (1954) Amer Chem Soc 76:4492
Yaron A, Berger A (1965) Biochim Biophys Acta 107:307
Sela M, Katachalsky E, Gehatia M (1956) J Amer Chem Soc 78:746
Van Holde E, Baldwin L (1958) J Phys Chem 62:734
Greenfield N, Fasman GD (1969) Biochemistry 8:4108
Woody RW (1977) J Polymer Sci Macromol Revs 12:181
Votavova H, Hudecz F, Kajtár J, Szekerke M, Šponar J, Blaha K (1980) Coll Czech Chem Commun 45:941
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A preliminary account of this work was read at the 4th Conference on Colloid Chemistry, Eger, 1983.
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Hudecz, F., Kovács, P., Kutassi-Kovács, S. et al. GPC, CD and sedimentation analysis of poly-Lys and branched chain poly-Lys-poly-DL-Ala polypeptides. Colloid & Polymer Sci 262, 208–212 (1984). https://doi.org/10.1007/BF01458962
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DOI: https://doi.org/10.1007/BF01458962