Abstract
The charge-transfer interaction of pyridinium salts with lysozyme and L-tryptophan has been investigated by visible absorption, circular dichroism and fluorescence spectroscopy. Four quaternized and substituted pyridinium salts were used as electron acceptors. L-tryptophan was used as a model compound of electron donor for the purpose of the comparison with lysozyme. The binding constants obtained from absorption and circular dichroism spectra were 2.0–13 M−1 and the sequence of the magnitude was found as EPCl(1-ethylpyridinium chloride) < MPCl(1-methylpyridinium chloride) < MNCl(1-methylnicotinamide chloride) < MNMCl(1-methyl-3-carbomethoxypyridinium chloride), which could be explained by the electron affinity of the pyridinium salt. The fluorescence spectra of lysozyme and L-tryptophan in the absence and presence of pyridinium salts were interpreted by Stern-Volmer plot. The static constants thus determined were in agreement with the binding constants obtained by other methods.
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Nakano Y, Seki T, Iwata H, Komiyama J, Iijima T (1982) Coll & Polym Sci 260:218
Nakano Y, Kawauchi S, Komiyama J, Iijima T (1982) Coll & Polym Sci 260:339
Slifkin MA (1971) Charge-transfer Interaction of Biomolecules, Academic Press, New York
Racker E, Krimsky IK (1952) J Biol Chem 198:731
Cilento G, Giusti P (1959) J Am Chem Soc 81:3801
Shifrin S (1964) Biochim Biophys Acta 81:205
Cilento G, Tedeschi P (1961) J Biol Chem 236:907
Ishida T, Tomita K, Inoue M (1980) Arch Biochem Biophys 200:492
Ishida T, Ibe S, Inoue M (1984) J Chem Soc Perkin Trans II:297
Deranleau DA, Bradshaw RA, Schwyzer R (1969) Proc Natl Acad Sci USA 63:885
Bradshaw RA, Deranleau DA (1970) Biochemistry 9:3310
Ikeda K, Hamaguchi K (1973) J Biochem (Tokyo) 74:221
Ikeda K, Hamaguchi K (1975) ibid 77:1
Karrer P, Schwarzenbach G, Benz F, Solmssen U (1936) Helv Chim Acta 19:811
Kosower EM (1955) J Am Chem Soc 77:3883
Sophianopoulous AJ, Rhodes CK, Holcomb DN, Van Holde KE (1962) J Biol Chem 237:1107
Kuramitsu S, Ikeda K, Hamaguchi K (1977) J Biochem (Tokyo) 83:159
Parker CA (1968) Photoluminescence of Solutions, Elsevier, New York, p 220
Deranleau DA (1969) J Am Chem Soc 91:4044
Ikeda K, Hamaguchi K (1973) J Biochem (Tokyo) 73:307
Deranleau DA, Schwyzer R (1970) Biochemsitry 9:126
Imoto T, Forster LS, Rupley JA, Tanaka F (1971) Proc Natl Acad Sci USA 69:1151
Lehrer SS, Fasman GD (1967) J Biol Chem 242:4644
Formoso C, Forster LS (1975) ibid 250:3738
Itani N, Kuramitsu S, Ikeda K, Hamaguchi K (1975) J Biochem, Tokyo 78:705
Kuramitsu S, Ikeda K, Hamaguchi K (1977) ibid 82:585
Stern O, Volmer M (1919) Phys Z 20:183
Birks JB (1970) Photophysics of Aromatic Molecules, Wiley-Interscience, New York, p 433
Eftink MR, Ghiron CA (1977) Biochemistry 16:5546
Eftink MR, Ghiron CA (1976) J Phys Chem 80:486
Smoluchowski NV (1917) Z Phys Chem 92:129
Frank IM, Vavilor SI (1931) Z Phys 69:100
Boaz H, Rollefson GK (1950) J Am Chem Soc 72:3435
Moon AY, Poland DC, Sheraga HA (1965) J Phys Chem 69:296
Vaughn WM, Weber G (1970) Biochemistry 9:464
Lakowicz JR, Weber G (1973) ibid 12:4161
Holms LG, Robbins FM (1974) Photochem Photobiol 19:361
Mulliken RS, Person WB (1969) Molecular Complexes, Wiley-Interscience, New York
I'haya Y (1955) Bull Chem Soc Jpn 28:369
Sakurai T, Hosoya H (1966) Biochim Biophys Acta 112:459
Yonezawa T, Nagata C, Kato H, Imamura A, Morokuma K (1964) Introduction to Quantum Chemistry (in Japanese), Kagaku-Dojin, Kyoto, p 55
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Nakano, Y., Komiyama, J. & Iijima, T. Charge-transfer interaction of some pyridinium salts with hen egg-white lysozyme. Colloid & Polymer Sci 265, 139–147 (1987). https://doi.org/10.1007/BF01412757
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DOI: https://doi.org/10.1007/BF01412757