Skip to main content
Log in

Histochemical study of normal human muscle spindle

Histochemical classification of intrafusal muscle fibers and intrafusal nerve endings

  • Published:
Journal of Neurology Aims and scope Submit manuscript

Summary

Normal muscle spindles of human skeletal muscle were studied histochemically. 1) Four histochemical types of intrafusal muscle fibers were classified by ATPase stain: Bag I fiber, Bag II fiber, Chain I fiber and Chain II fiber. Moreover, two types of nuclear bag fibers were classified by NADH Tetrazolium Reductase stain and PAS stain: Bag I fiber and Bag II fiber. 2) Three kinds of fusimotor endings were verified by the cholinesterase technic: en plaque, en grappe and diffuse endings. 3) Two kinds of fusisensory endings were verified by NADH TR stain and also electron-microscopically: primary and secondary sensory endings.

Zusammenfassung

Es wurden normale Skeletmuskelspindeln von Menschen histochemisch untersucht. 1. Histochemisch wurden vier Muskelfasertypen der intrafusalen Fasern — Bag I fiber, Bag II fiber, Chain I fiber und Chain II fiber — von ATPase-Aktivität klassifiziert. Bei der NADH-Tetrazolium-Reduktase wurden zwei Muskelfasertypen an intrafusalen Fasern — Bag I fiber und Bag II fiber — beobachtet. 2. Drei Typen der motorischen Endplatte — en plaque Endplatte, en grappe Endplatte und diffuse Endplatte — wurden an Muskelspindeln von Cholinesterase klassifiziert. 3. Zwei Typen der sensorischen Nervenenden — primär-sensorisches Nervenende und sekundärsensorisches Nervenende — wurden an Muskelspindeln mit NADH-Tetrazolium-Reduktase und elektronmiktroskopisch beobachtet.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • De Anda, G., Rebollo, M. A.: The neuromuscular spindles in the adult chicken; I. Morphology. Acta Anat. 67, 437–451 (1967)

    Google Scholar 

  • Banker, B. Q., Girvin, J. P.: The ultrastructural features of the normal and de-efferented mammalian muscle spindle, pp. 267–298. In: Research in muscle development and the muscle spindle. (Banker, B. Q., Prybylski, R. J., Van Der Meulen, J. P., Victor, M., eds.). Amsterdam: Excerpta Medica 1972

    Google Scholar 

  • Barker, D., Harker, D., Stacey, M. J., Smith, C. R.: Fusimotor innervation, pp. 227–253. In: Research in muscle development and the muscle spindle (Banker, B. Q., Przybylski, R. J., Van Der Meulen, J. P., Victor, M., eds.). Amsterdam: Excerpta Medica 1972

    Google Scholar 

  • Cöers, C., Durand, J.: Données morphologiques nouvelles sur l'innervation des fuseaux neuromusculaires. Arch. Biol. 67, 685–786 (1956)

    Google Scholar 

  • Copper, S., Daniel, P. M.: Muscle spindles in man; their morphology in lumbricals and the deep muscles of the neck. Brain 86, 563–586 (1963)

    Google Scholar 

  • Dorn, A.: Enzymehistochemische Befunde an Muskelspindeln. Acta Histochem. 31, 14–23 (1968)

    Google Scholar 

  • Engel, W. K., Cunningham, G. G.: An improved trichrome method for rapid diagnosis of muscle biopsy fresh-frozen sections. Neurology 13, 919–923 (1963)

    Google Scholar 

  • Gruner, J. E.: La structure fine du fuseau neuromusculaire humain. Rev. Neurol. 104, 490–507 (1961)

    Google Scholar 

  • Hess, A.: Two kinds of motor nerve endings on mammalian intrafusal muscle fibres revealed by the cholinesterase technique. Anat. Rec. 139, 174–184 (1961)

    Google Scholar 

  • James, N. T.: Histochemical demonstration of myoglobin in skeletal muscle fibres and muscle spindle. Nature 219, 1174–1175 (1970)

    Google Scholar 

  • Kennedy, W. R.: Innervation of normal human muscle spindle. Neurology 20, 463–475 (1970)

    Google Scholar 

  • Khan, M. A., Papadimitriou, J. M., Holt, P. G., Kakulas, B. A.: A calcium-citro-phosphate technique for the histochemical localization of myosin ATPase. Stain Technology 47, 277–281 (1972)

    Google Scholar 

  • Koelle, G. B.: The elimination of enzymatic diffusion artifacts in the histochemical localization of cholinesterase and a survey of their cellular distributions. J. Pharma. Exp. Therap. 103, 153–171 (1951)

    Google Scholar 

  • Kupffer, C.: Motor innervation of extraocular muscle. J. Physiol. 153, 522–526 (1960)

    Google Scholar 

  • Maier, A., Simpson, D. R., Edgerton, V. R.: Histochemical profiles of guinea-pig intrafusal fibres in normal and after denervation, cordotomy and tenotomy. Histochem. J. 6, 665–677 (1974)

    Google Scholar 

  • Merrilees, N. C. R.: The fine structure of muscle spindles in the lumbrical muscles of the rat. J. Biophys. Biochem. Cytol. 7, 725–742 (1960)

    Google Scholar 

  • Nakajima, Y., Shamta, T. R., Bourne, G. H.: Enzyme-histochemical studies on the muscle spindle. Histochemie 16, 1–8 (1968)

    Google Scholar 

  • Nyström, B.: Muscle spindle histochemistry. Science 155, 1424–1426 (1967)

    Google Scholar 

  • Ogata, T., Mori, M.: Histochemical demonstration of the three types of intrafusal fibres of muscle spindles; A study on oxidative enzymes. Acta Med. Okayama 16, 347–350 (1962)

    Google Scholar 

  • Rebollo, M. A., De Anda, G.: The neuromuscular spindles in the adult chicken; II. Histochemistry. Acta Anat. 67, 595–608 (1968)

    Google Scholar 

  • Ruffini, A.: Observation on sensory nerve-endings in voluntary muscle. Brain 20, 368–374 (1897)

    Google Scholar 

  • Sahgal, V., Morgen, C. A.: Histochemical and morphological changes in human muscle spindle in upper and lower motor neuron lesions. Acta neuropath. (Berl.) 34, 41–46 (1976)

    Google Scholar 

  • Soukup, T.: Intrafusal fibre types in rat limb muscle spindles; Morphological and histochemical characteristics. Histochemistry 47, 43–57 (1976)

    Google Scholar 

  • Spiro, A. J., Beilin, R. L.: Human muscle spindle histochemistry. Arch. Neurol. 20, 271–275 (1969a)

    Google Scholar 

  • Spiro, A. J., Beilin, R. L.: Histochemical duality of rabbit intrafusal fibres. J. Histochem. Cytochem. 17, 271–275 (1969b)

    Google Scholar 

  • Wirsen, C.: Histochemical heterogeneity of muscle spindle fibres. J. Histochem. Cytochem. 12, 308–309 (1964)

    Google Scholar 

  • Yellin, H.: A histochemical study of muscle spindles and their relationship to extrafusal fibre types in the rat. Am. J. Anat. 125, 31–46 (1969a)

    Google Scholar 

  • Yellin, H.: Unique intrafusal and extraocular muscle fibres exhibiting dual actomyosin ATPase activity. Exp. Neurol. 25, 153–163 (1969b)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saito, M., Tomonaga, M., Hirayama, K. et al. Histochemical study of normal human muscle spindle. J. Neurol. 216, 79–89 (1977). https://doi.org/10.1007/BF00312942

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00312942

Key words

Navigation