Abstract
We investigated the effect of chronic mechanical compression of the cervical spinal cord on the number of spinal accessory motoneurons in 25 tiptoe-walking Yoshimura mice. The animals had calcified deposits in the atlantoaxial membrane at the C1-C2 vertebral level, compressing the spinal cord posterolaterally. Motoneurons of the spinal accessory nerve between C1 and C5 segments were labelled using wheat germ agglutinin-horseradish peroxidase (WGA-HRP) injected into the sternocleidomastoid muscles. The counted cells were processed into a three-dimensional computer display to analyse the cytoarchitectonic changes caused by external cord compression. The number of WGA-HRP-labelled spinal accessory motoneurons was significantly reduced on the affected side. The number of motoneurons in compromised C2 and C3 cord segments correlated linearly with the extent of mechanical compression, but no such relationship was present on the contralateral side. There was an increase in the number of WGA-HRP-labelled spinal accessory motoneurons in the medial cell pools of the anterior grey horn at a level most rostral to the compression, and in the ventrolateral cell pools at levels immediately rostral to the compression. Our findings suggest that the spinal accessory motoneurons translocate rostral to the area of external compression in order to avoid mechanical injury.
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Al-Mefty O, Harkey HL, Marawi I, Haines DE, Peeler DF, Wilner HI, Smith RR, Haladay HR, Haining JL, Russel WF, Harrison B, Middleton TY (1993) Experimental chronic compressive myelopathy. J Neurosurg 79: 550–561
Baba H, Kawahara N (1995) Spinal cord evoked potential monitoring in orthopaedic spinal surgery. In: Dimitrijevic MR, Halter JA (eds) Atlas of human spinal cord evoked potentials. Butterworth Heinemann, Boston, pp 123–131
Baba H, Maezawa Y, Kawahara N, Tomita K, Furusawa N, Imura S (1993) Calcium crystal deposition in the ligamentum flavum of the cervical spine. Spine 18: 2174–2181
Baba H, Furusawa N, Tanaka Y, Wada M, Imura S, Tomita K (1994) Anterior decompression and fusion for cervical myeloradiculopathy secondary to ossification of the posterior longitudinal ligament. Int Orthop 18: 204–209
Baba H, Furusawa N, Chen Q, Imura S, Tomita K (1995) Anterior decompressive surgery for cervical ossified posterior longitudinal ligament causing myeloradiculopathy. Paraplegia 33: 18–24
Baba H, Furusawa N, Chen Q, Imura S (1995) Cervical laminoplasty in patients with ossification of the posterior longitudinal ligaments. Paraplegia 33: 25–29
Baba H, Imura S, Kawahara N, Nagata S, Tomita K (1995) Osteoplastic laminoplasty for cervical myeloradiculopathy secondary to ossification of the posterior longitudinal ligament. Int Orthop 19: 40–45
Baba H, Chen Q, Uchida K, Imura S, Morikawa S, Tomita K (1996) Laminoplasty with foraminotomy for coexisting cervical myelopathy and unilateral radiculopathy: a preliminary report. Spine 21: 196–202
Baba H, Maezawa Y, Imura S, Kawahara N, Nakahashi K, Tomita K (1996) Quantitative analysis of the spinal cord motoneuron under chronic compression: an experimental observation in the mouse. J Neurol 243: 109–116
Baba H, Maezawa Y, Imura S, Kawahara N, Tomita K (1996) Spinal cord evoked potential monitoring for cervical and thoracic compressive myelopathy. Paraplegia 34: 100–106
Barber RP, Phelps PE, Houser CR, Crawford GD, Salvaterra RP, Vaughn JE (1984) The morphology and distribution of neurons containing choline acetyltransferase in the adult rat spinal cord: an immunohistochemical study. J Comp Neurol 229: 329–346
Benzel EC, Lancon JA, Thomas MM, Beal JA, Hoffpauir GM, Kesterson L (1990) A new rat spinal cord injury model: a ventral compression technique. J Spinal Disord 3: 334–338
Berghausen EJ, Balogh K, Landis WJ, Lee DD, Wright AM (1987) Cervical myelopathy attributable to pseudogout. Case report with radiologic, histologic, and crystallographic observations. Clin Orthop 214: 217–221
Brichta AM, Callister BJ, Peterson EH (1987) Quantitative analysis of cervical musculature in rats: histochemical composition and motor pool organization. I. Muscles and the spinal accessory complex. J Comp Neurol 255: 351–368
Fujiwara K, Yonenobu K, Hiroshima K, Ebara S, Yamashita K, Ono K (1988) Morphometry of the cervical spinal cord and its relation to pathology in cases with compression myelopathy. Spine 13: 1212–1216
Fukushima T, Ikata T, Taoka Y, Takata S (1991) Magnetic resonance imaging study on spinal cord plasticity in patients with cervical compression myelopathy. Spine 16 [Suppl]: S534-S538
Hankey G, Khangure MS (1988) Cervical myelopathy due to calcification of the ligamentum flavum. Aust N Z J Surg 58: 247–249
Harkey HL, Al-Mefty O, Marawi I, Peeler DF, Haines DE, Alexander LF (1995) Experimental chronic compressive cervical myelopathy: effect of decompression. J Neurosurg 83: 336–341
Hashizume Y, Iijima S, Hirano A (1983) Pencil-shaped softening of the spinal cord. Acta Neuropathol (Berl) 61: 219–224
Hashizume Y, Iijima S, Kishimoto H, Yanagi T (1984) Pathology of spinal cord lesions caused by ossification of the posterior longitudinal ligament. Acta Neuropathol (Berl) 63: 123–130
Hosoda Y, Yoshimura Y, Higaki S (1981) A new breed mouse showing multiple osteochondral lesions: the twy mouse. Ryumachi (Tokyo) 21: 157–164
Hukuda S, Wilson CB (1972) Experimental cervical myelopathy: effect of compression and ischemia on the canine cervical cord. J Neurosurg 37: 631–652
Imai S, Hukuda S (1994) Cervical radiculomyelopathy due to deposition of calcium pyrophosphate dihydrate crystals in the ligamentum flavum: historical and histological evaluation of attendant inflammation. J Spinal Disord 7: 513–517
Kameyama T, Hashizume Y, Ando T, Takahashi A (1994) Morphometry of the normal cadaveric cervical spinal cord. Spine 19: 2077–2081
Kameyama T, Hashizume Y, Ando T, Takahashi A, Yanagi T, Mizuno J (1995) Spinal cord morphology and pathology in ossification of the posterior longitudinal ligament. Brain 118: 263–278
Kitamura S, Sakai A (1982) A study on the localization of the sternocleidomastoid and trapezius motoneurons in the rat by means of the HRP method. Anat Rec 202: 527–536
Kitamura S, Sakai A, Nishiguchi T (1980) A cytoarchitectonic study of the calcification of the ventral horn cell groups in the rat cervical spinal cord. J Osaka Univ Dent Sch 25: 186–202
Kojimahara K, Sugiura H, Kanai Y, Kameyama K, Hosoda Y, Shibata T, Ogawa Y (1992) Vertebral and spinal cord lesions of twy mice with genetic osteochondral abnormalities. In: Kurokawa T (ed) Investigation Committee Report on the Ossification of the Spinal Ligaments. Ministry of Health and Welfare of Japan, Tokyo, pp 46–50
Martin D, Schoenen J, Delrée P, Gilson V, Rogister B, Leprince P, Stevenaert A, Moonen G (1992) Experimental acute traumatic injury of adult rat spinal cord by a subdural inflatable balloon: methodology, behavioral analysis, and histopathology. J Neurosci Res 32: 539–550
McAfee PC, Regan JJ, Bohlman HH (1987) Cervical cord compression from ossification of the posterior longitudinal ligament in non-orientals. J Bone Joint Surg [Br] 69: 569–575
McClung JR, Castro AJ (1978) Rexed’s laminar scheme as it applies to the rat cervical spinal cord. Exp Neurol 58: 145–148
Mesulam MM (1978) Tetramethylben zidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26: 106–117
Miyasaka K, Kaneda K, Sato S (1983) Myelopathy due to ossification or calcification of the ligamentum flavum: radiologic and histologic evaluation. Am J Neuroradiol 4: 629–632
Mizuno J, Nakagawa H, Iwata K, Hashizume Y (1992) Pathology of spinal cord lesions caused by ossification of the posterior longitudinal ligament, with special reference to reversibility of the spinal cord lesion. Neurol Res 14: 312–314
Nishiguchi T, Kitamura S, Okubo J, Ogata K, Sakai A (1986) Location of rabbit spinal accessory nucleus: a study by means of the HRP method. J Osaka Univ Dent Sch 26: 51–58
Okada Y, Ikata K, Katoh S, Yamada H (1994) Morphologic analysis of the cervical spinal cord, durai tube and spinal canal by magnetic resonance imaging in normal adults and patients with cervical spondylotic myelopathy. Spine 19:2331–2335
Prestor B, Zgur T, Dolenc VV (1993) Incomplete spinal cord evoked injury potential in man. Spine 18: 252–256
Rapoport S (1978) Location of sternocleidomastoid and trapezius motoneurons in the rat. Brain Res 156: 339–344
Saito H, Mimatsu K, Sato K, Hashizume Y (1992) Histopathologic and morphometric study of spinal cord lesion in a chronic cord compression model using bone morphogenetic protein in rabbits. Spine 17: 1368–1374
Schramm J, Krause R, Shigeno T, Brock M (1983) Experimental investigation on the spinal cord evoked injury potential. J Neurosurg 59: 485–492
Sherman JL, Nassaux PY, Citrin CM (1990) Measurements of the normal cervical spinal cord on MR imaging. Am J Neuroradiol 11: 369–372
Shinomiya K, Mutoh N, Furuya K (1992) Study of experimental cervical spondylotic myelopathy. Spine 17 [Suppl]: S383-S387
Steiner TJ, Turner LM (1972) Cytoarchitecture of the rat spinal cord. J Physiol (Lond) 222: 123–124
Sypert GW, Arpin-Sypert EJ (1993) Ossification of the posterior longitudinal ligament. In: Whitecloud TS III, Dunsker SB (eds) Anterior cervical spine surgery. Raven Press, New York, pp 105–118
Terakado A, Tagawa M, Goto S, Yamazaki M, Moriya H, Fujimura S (1995) Elevation of alkaline phosphatase activity induced by parathyroid hormone in osteoblast-like cells from the spinal hyperostotic mouse TWY (twy/twy). Calcif Tissue Int 56: 135–139
Thijssen HOM, Keyser A, Horstink MWM, Meijer E (1979) Morphology of the cervical spinal cord on computed myelography. Neuroradiology 18: 57–62
Tomita K, Nomura S, Umeda S, Baba H (1988) Cervical laminoplasty to enlarge the spinal canal in multilevel ossification of the posterior longitudinal ligament with myelopathy. Arch Orthop Trauma Surg 107: 148–153
Yamada M, Yoshizawa H, Kobayashi S, Shibayama T, Ukai T, Nakagawa M, Fujiwara Y, Morita C (1992) Fine structure of the twy mouse spinal cord. In: Kurokawa T (ed) Investigation Committee Report on the Ossification of the Spinal Ligaments. Ministry of Health and Welfare of Japan, Tokyo, pp 46–50
Yamazaki M, Moriya H, Goto S, Saito S, Arai K, Nagai Y (1991) Increased type XI collagen expression in the spinal hyperostotic mouse (twy/twy). Calcif Tissue Int 48: 182–189
Yato Y, Fujimura Y, Nakamura M, Watanabe M, Hirabayashi K, Hosoda Y (1995) Immunohistochemical study on changes of anterior horn cell in twy mouse. In: Sakou T (ed) Investigation Committee Report on the Ossification of the Spinal Ligaments. Ministry of Health and Welfare of Japan, Tokyo, pp 105–106
Zheng C, Heintz N, Hatten ME (1996) CNS gene encoding astrotactin, which supports neuronal migration along glial fibers. Science 272: 417–419
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Baba, H., Maezawa, Y., Uchida, K. et al. Three-dimensional topographic analysis of spinal accessory motoneurons under chronic mechanical compression: an experimental study in the mouse. J Neurol 244, 222–229 (1997). https://doi.org/10.1007/s004150050076
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DOI: https://doi.org/10.1007/s004150050076