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Application of thin layer and high performance liquid chromatography to the separation and determination of cephalexin in cephradine, in bulk powder and in pharmaceuticals

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Summary

A thin-layer chromatographic procedure for the quantitative determination of cephalexin in cephradine formulations is described. The thin-layer chromatography is carried out on a silica gel layer and the quantitation is by UV scanning of the chromatogram. The procedure was transferred to high-performance liquid chromatography on a microparticulate silica gel column with a similar eluent system. The cephalexin content of a few commercial cephradine samples is determined by using these techniques. The results of these procedures are compared to those obtained with high-performance liquid chromatography on a C 18 reverse phase column.

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References

  1. J. E. Dolfini, H. E. Applegate, G. Bach, H. Basch, J. Bernstein, J. Schwartz andF. L. Weisenborn, J. Med. Chem.14, 117 (1971).

    Article  PubMed  Google Scholar 

  2. C. W. Ryan, R. L. Simon andE. M. Van Heyningen, J. Med. Chem.12, 310 (1969).

    Article  PubMed  Google Scholar 

  3. V. Hartmann andM. Rödiger, Chromatographia9, 266 (1976).

    Google Scholar 

  4. British Pharmacopoeia, London, 1973.

  5. B. M. Frantz, J. Pharm. Sci.65, 887 (1976).

    PubMed  Google Scholar 

  6. A. F. Heald, C. E. Ita andE. C. Schreiber, J. Pharm. Sci.65, 768 (1976).

    PubMed  Google Scholar 

  7. A. B. C. Yu, C. H. Nightingale andD. R. Flanagan, J. Pharm. Sci.66, 213 (1977).

    PubMed  Google Scholar 

  8. L. P. Marelli, J. Pharm. Sci.61, 1647 (1972).

    PubMed  Google Scholar 

  9. J. Kirchbaum, J. Pharm. Sci.63, 923 (1974).

    Google Scholar 

  10. D. L. Mays, F. K. Bangert, W. C. Cantrell andW. G. Evans, Anal. Chem.47, 2229 (1975).

    Article  PubMed  Google Scholar 

  11. A. Zaki, E. C. Schreiber, I. Weliky, J. R. Knill, J. A. Hubsher, J. Clin. Pharmacol., New Drugs14, 118 (1974).

    Google Scholar 

  12. “Analytical Profiles of Drug Substances”, Vol. 5, Ed.K. Florey, Academic Press, New York, p. 22, 1976.

    Google Scholar 

  13. M. A. Carroll, E. R. White, Z. Jancsik andJ. E. Zarembo, J. Antibiot.30, 397 (1977).

    PubMed  Google Scholar 

  14. E. R. White, M. A. Carroll andJ. E. Zarembo, J. Antibiot.30, 811 (1977).

    PubMed  Google Scholar 

  15. T. S. Chou, J. Med. Chem.9, 925 (1969).

    Article  Google Scholar 

  16. H. Schlitt andF. Geiss, J. Chromatogr.67, 261 (1972).

    Article  Google Scholar 

  17. E. A. Mac Mullan andJ. E. Heveran in “Quantitative thin layer chromatography”,J. C. Touchstone (Editor), Wiley Interscience, New York, 1973, p. 217.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry and Drug Quality Control, State University of Ghent, Faculty of Pharmaceutical Sciences, De Pintelaan 135, B-9000, Ghent, Belgium

    E. Crombez, G. A. Bens, G. Van der Weken, W. Van den Bossche & P. De Moerloose

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  1. E. Crombez
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  2. G. A. Bens
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  3. G. Van der Weken
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  4. W. Van den Bossche
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  5. P. De Moerloose
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Crombez, E., Bens, G.A., Van der Weken, G. et al. Application of thin layer and high performance liquid chromatography to the separation and determination of cephalexin in cephradine, in bulk powder and in pharmaceuticals. Chromatographia 11, 653–657 (1978). https://doi.org/10.1007/BF02269000

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  • DOI: https://doi.org/10.1007/BF02269000

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