Summary
A new method has been developed for the determination of phenol in urine in which the phenol conjugates are hydrolysed enzymatically and the liberated phenol is analysed by headspace chromatography. The results compare favourably with those obtained by the method of van Haaften and Sie in which acid hydrolysis is carried out in a heated GC precolumn. The enzymatic hydrolysis headspace technique appears to be very reliable and does not suffer from the disadvantages of precolumn acid hydrolysis.
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References
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Since this paper was submitted, we have found a report on the enzymatic hydrolysis of phenyl glucuronide in urine followed by gas chromatographic analysis which seems to have been overlooked by later workers. The paper is by H. Desoille, T. F. Gandenzi-Britto and M. Philbert in Archives des Maladies Professionelles, de Medecine du Travail et de Securite Sociale (Paris),30 (3), 129 (1969).
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Adlard, E.R., Milne, C.B. & Tindle, P.E. The determination of phenol in urine by enzymatic hydrolysis/headspace gas chromatography. Chromatographia 14, 507–509 (1981). https://doi.org/10.1007/BF02265629
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DOI: https://doi.org/10.1007/BF02265629